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The map label for this gene is topB2 [H]

Identifier: 218230874

GI number: 218230874

Start: 1820770

End: 1822914

Strand: Direct

Name: topB2 [H]

Synonym: BCB4264_A1911

Alternate gene names: 218230874

Gene position: 1820770-1822914 (Clockwise)

Preceding gene: 218235463

Following gene: 218233042

Centisome position: 33.6

GC content: 36.97

Gene sequence:

>2145_bases
ATGAAATTAATTATTGCCGAGAAACCAGATCAAGGGTTGGCTCTTGTTTCACAGTTTAAATATCGCCGGAAAGATGGATA
TTTAGAAGTAGAAGCGAATGAGTTATTTCCAAATGGAGCGTACTGTACATGGGCAATTGGTCATTTGACGCAGTTATGTA
ATCCAGAGCATTATCACGCAGAGTGGAAAAAATGGTCACTTGATACGTTACCGATGATTCCTGAACGATTTCAATTTGAA
GTAACAAAGTCAAAGTATAAGCAATTTAACGTTGTGAAACAGCTGTTACATAATCCACAGGTAACAGAAATCATTCACGC
AGGAGATGCTGGGCGTGAAGGGGAACTGATCGTACGAAATATTATTAATCTTTGTAACGTGCAAAAGCCGATGAAACGTC
TATGGATTTCATCTTTAACGAAGCAAGCTATTTACCAAGGATTTAAAAATCTACTTGATGAATCAGATACAATTAATACG
TATTACGAAGCATACACAAGATCTTGCGCTGACTGGGTTGTTGGTATGAATGCATCACGTGTCTTTAGTATTTTGTTAAA
GAAAAAAGGAATGAATGATGTATTTTCTGCTGGTCGTGTCCAAACACCAACGTTAGCATTGATTGTAAAGCGAGAGAAAG
AAATTGAAAACTTTAAGTCAGAGCCTTTCTGGGAAGTGTTTGCAACCTTTAATATAGAAGGAAAGAAATATGACGGGAAA
TGGGAAAAGGATAATGAATCCCGCTTAAAAGACCCTGATATGGCGAATAAAATTGCGGCATTTTGCCAAGGGAAACCGGC
TGTTGTGAAGGAAATGAAAACGGAGCGTAAAGAGTTTCAGCCGCCGCTTTTATTTAACTTATCATCACTGCAAGCAACGG
CAAATAAAGCCTTTAAGTTTTCACCGAAAAAGACGCTTGATATAACGCAAGCACTATATCAAAAAGGGATTGTTTCTTAT
CCACGTTCAGATTCTAACTATGTTACACAAGGAGAAGCAGCGACGTTCCCTGATATTTTACAGAAGTTAAGTCAGTTTGA
TGAATATAAAGGCTTATTACCGGCTCCGGTTGAATCCATTATGAATAACAAGCGTTATGTAAATGAAAAGAAAGTAACAG
ATCACTACGCAATTATTCCGACAGAGCAAGTTACAAACCCAAGCAGACTATCAGGTGATGAAAAGAAAATATACGATATG
ATCGTAAGAAGGCTTATAGCAGCTCATTATGAAGTTGCAATCTTTGACTATACAACGATTGTAACGCTTGTAGATGAACG
TGCTGAATTCATTTCAAAAGGAAAACAGCAAATTCAAGAAGGTTGGCGTAAAGTTATTTTCCAAGACGATAAAGATGACG
AAACCATTCTTCCAATTGTAGCTGAAGGCGAAGAAGGAAAAGTTGTAAAGGTGAAAGTGAAAGAAGGAAAAACACAGCCA
CCGAAGCGCTATACAGAAGGACAACTTATTACGTTAATGAAAACAGCTGGTAAGTATTTAGAGAATGAAGAGCTGGAGAA
AGTATTAAAGAAAACAGAAGGTTTAGGTACGGAAGCGACTCGCGCAGGTATTATTACGATGCTGAAAGACCGTAAGTATA
TAGATGTAAAGAAAAACCAAGTGTATGCGACTGATAAAGGAAAAGTATTAATTACCGCAATTGGTGACAAAATACTAGCT
TCACCAGAAATGACTGCGAAATGGGAACAACGTCTTGCGGAAATTGGTGAAGGTACAGCTTCACCAGCTACATTTATGGA
ACAGACGAAAAAGCTATCAGCTAAAATTATTGAAGATGCAGTGGAAATGTCAGAGAAATGGGACTTTACTGGATTGCATG
TTGAATCGATTGAGCGAAAAGGATCGAAATTTACAACAGGTAAAAAGGTTGGTAGCTGTAAAAAATGTGATGGCGATGTA
ATTGATAAGTCAACGTTTTACGGCTGTTCTAATTACAACACGACACAATGTGACTTTACCATCTCGAAGAAAATATTAAG
TAAAACAATTTCGCAAAAGAATATGACAAAGCTATTAAGAGGTGAAAAGACCGATTTAATTAAAGGCTTTAAAAAGGGCG
AGAAAACATTTGATGCGAAGTTAGAGTGGAAAGATAATAAGATTAATTTTGTGTTTGAGAATTAA

Upstream 100 bases:

>100_bases
GAGACAAACCGCGAATGATAAATAAGATTGTTGTTTTATCATCGCTTCTAAACATATAGACAATGAAAGTAACACTGGAA
TACAGATAGGGAGTTATAAA

Downstream 100 bases:

>100_bases
GTTGTTTTAACAAATAAAAATATGATAATTAAAAAATATCCTATAGAATAGACGCTTAAAAAAGTCTATTCTATAGGATA
TTTTTTTGAATAAGTAGTAC

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
WEKDNESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLRGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Sequences:

>Translated_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
WEKDNESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLRGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
>Mature_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
WEKDNESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLRGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=673, Percent_Identity=24.2199108469539, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI4507635, Length=458, Percent_Identity=25.764192139738, Blast_Score=106, Evalue=8e-23,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=33.7641357027464, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1787529, Length=518, Percent_Identity=28.1853281853282, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=27.7955271565495, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=497, Percent_Identity=25.3521126760563, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=629, Percent_Identity=25.9141494435612, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=702, Percent_Identity=24.6438746438746, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24640096, Length=493, Percent_Identity=23.9350912778905, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81532; Mature: 81532

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDNESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCHHCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
VTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLRGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCEEEEEECC
>Mature Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPNGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCEEEHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDNESRLKDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCHHCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
VTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLRGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]