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The map label for this gene is ispD [H]

Identifier: 218230825

GI number: 218230825

Start: 101583

End: 102263

Strand: Direct

Name: ispD [H]

Synonym: BCB4264_A0106

Alternate gene names: 218230825

Gene position: 101583-102263 (Clockwise)

Preceding gene: 218231556

Following gene: 218233135

Centisome position: 1.87

GC content: 40.38

Gene sequence:

>681_bases
ATGTATACATTAATTATTCCGGCAGCGGGCCAAGGAAAACGAATGGGTGCTGGTAAAAATAAGTTATTTTTACTTATAGA
TGAAGTACCAATTATTGTGCACACATTGCGTGCTTTTGAAAAAGATAAAGCGTGCAAAAGTATTATTATGGCAATTAACG
AAGAAGAACGCCCGTATTTTGAAGAGTTAATGCAGAAGTACCAGATTGAAAAGCACGTACAATTCATTCAGGGTGGAGCC
GAAAGACAAGATAGCGTCTATAACGCACTTCAGTATGCGAGTGGTGTCGAATATGTTCTTGTGCATGACGGGGCGCGCCC
GTTCGTAACGAATAAAGTGATTCGCGATGTATTAACTGCAGCAGAAAAATATGGAGCTTCCATTTGTGCAGTGCCAGTAA
AAGATACTGTTAAGAAAGTAGAGAAGGGTGTTGTTGTAGAAACGGTAGAGCGATCTCAACTTAACGCTGTACAAACACCA
CAAGGATTCTCTGTTTCTCTTTTGCTAGAAGCTCATAGAAGCGCAAAGCAAAGTTGTTTTCTTGGCACAGATGATGCAAG
TCTCGTAGAACGTGTGGGGAAGCAAGTAGGTGTAGTAGAGGGGAGTTACTATAATATTAAAGTGACGACTCCGGAAGATC
TATTAATTGCTGAAAGTTTCCTACGTGTTCAGAAGAAATAA

Upstream 100 bases:

>100_bases
GACGTACTTGTTACTAGCGTATTACAAACATCGGCTGGTCGTATGATTTTCGCAAAACGTAAATTGCTAGAAAAAGCATT
ATAAGTAGAGGATTATTAAT

Downstream 100 bases:

>100_bases
CATTGATGACGTGTTAATAAAGAAAAGCTCGGCAACAGCCGGGCTTTTCTTTATAAGGATAGCGATATAATATAGAGTCA
TAAAGCTCAGTAGTTTAGCT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLIDEVPIIVHTLRAFEKDKACKSIIMAINEEERPYFEELMQKYQIEKHVQFIQGGA
ERQDSVYNALQYASGVEYVLVHDGARPFVTNKVIRDVLTAAEKYGASICAVPVKDTVKKVEKGVVVETVERSQLNAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERVGKQVGVVEGSYYNIKVTTPEDLLIAESFLRVQKK

Sequences:

>Translated_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLIDEVPIIVHTLRAFEKDKACKSIIMAINEEERPYFEELMQKYQIEKHVQFIQGGA
ERQDSVYNALQYASGVEYVLVHDGARPFVTNKVIRDVLTAAEKYGASICAVPVKDTVKKVEKGVVVETVERSQLNAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERVGKQVGVVEGSYYNIKVTTPEDLLIAESFLRVQKK
>Mature_226_residues
MYTLIIPAAGQGKRMGAGKNKLFLLIDEVPIIVHTLRAFEKDKACKSIIMAINEEERPYFEELMQKYQIEKHVQFIQGGA
ERQDSVYNALQYASGVEYVLVHDGARPFVTNKVIRDVLTAAEKYGASICAVPVKDTVKKVEKGVVVETVERSQLNAVQTP
QGFSVSLLLEAHRSAKQSCFLGTDDASLVERVGKQVGVVEGSYYNIKVTTPEDLLIAESFLRVQKK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=230, Percent_Identity=32.1739130434783, Blast_Score=106, Evalue=1e-23,
Organism=Homo sapiens, GI157671913, Length=132, Percent_Identity=35.6060606060606, Blast_Score=85, Evalue=4e-17,
Organism=Escherichia coli, GI1789104, Length=220, Percent_Identity=34.5454545454545, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25123; Mature: 25123

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLIDEVPIIVHTLRAFEKDKACKSIIMAINEEERPYF
CEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EELMQKYQIEKHVQFIQGGAERQDSVYNALQYASGVEYVLVHDGARPFVTNKVIRDVLTA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
AEKYGASICAVPVKDTVKKVEKGVVVETVERSQLNAVQTPQGFSVSLLLEAHRSAKQSCF
HHHHCCEEEEECHHHHHHHHHCCHHEEEHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHEE
LGTDDASLVERVGKQVGVVEGSYYNIKVTTPEDLLIAESFLRVQKK
CCCCCHHHHHHHHHHHCEEECCEEEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTLIIPAAGQGKRMGAGKNKLFLLIDEVPIIVHTLRAFEKDKACKSIIMAINEEERPYF
CEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EELMQKYQIEKHVQFIQGGAERQDSVYNALQYASGVEYVLVHDGARPFVTNKVIRDVLTA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHH
AEKYGASICAVPVKDTVKKVEKGVVVETVERSQLNAVQTPQGFSVSLLLEAHRSAKQSCF
HHHHCCEEEEECHHHHHHHHHCCHHEEEHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHEE
LGTDDASLVERVGKQVGVVEGSYYNIKVTTPEDLLIAESFLRVQKK
CCCCCHHHHHHHHHHHCEEECCEEEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA