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The map label for this gene is ispF [H]

Identifier: 218233135

GI number: 218233135

Start: 102380

End: 102856

Strand: Direct

Name: ispF [H]

Synonym: BCB4264_A0107

Alternate gene names: 218233135

Gene position: 102380-102856 (Clockwise)

Preceding gene: 218230825

Following gene: 218234593

Centisome position: 1.89

GC content: 37.74

Gene sequence:

>477_bases
ATGTTTCGAATTGGACAAGGTTTTGATGTACATGAATTTGCGGAAGGGCGCCCGTTGATTATCGGTGGAATTACAATTCC
ACACGAGAAAGGATTAATCGGTCATTCAGATGCAGATGTATTGTTACATACGATTGCTGATGCATGTTTAGGTGCGATTG
CAGCAGGAGATATCGGAAAGCATTTTCCTGATACAGACCCAGCTTTTAAAGATGCAGATTCAGCTGTATTGTTACAAAAA
GTTTGGGGATTTGTGCGTGAACAAGGTTACGAGTTAGGAAATTTAGATTGTACAATTATTGCTCAAAAACCAAAAATGGC
ACCACATATTGAGAGTATGCGTAAGCGCATTAGTGAACTGTTAGAAACGTCTATCGATAACATTAATGTAAAGGCAACAA
CAACAGAAAAATTAGGATTTACAGGTAGAGAAGAAGGGATTGCTTCTCAAGCGGTTGTTTTATTACAGAAAAAATAA

Upstream 100 bases:

>100_bases
ATAAAGAAAAGCTCGGCAACAGCCGGGCTTTTCTTTATAAGGATAGCGATATAATATAGAGTCATAAAGCTCAGTAGTTT
AGCTTAAGGAGGAAGTAAGG

Downstream 100 bases:

>100_bases
TGGTTTTTAATTCGTAAGATGGATAATCACATTTTTTTGGTGTACAATTATAGAATGTGTTGACATTAAGAATGGAAGGT
GTACCAATTATGGAAAAGCA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWGFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK

Sequences:

>Translated_158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWGFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
>Mature_158_residues
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGKHFPDTDPAFKDADSAVLLQK
VWGFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISELLETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=64.1025641025641, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17147; Mature: 17147

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGK
CCCCCCCCCHHHHCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
HFPDTDPAFKDADSAVLLQKVWGFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
HHHHHCCCEEEEECHHHCCCCCCCCCHHHHEEEEEECC
>Mature Secondary Structure
MFRIGQGFDVHEFAEGRPLIIGGITIPHEKGLIGHSDADVLLHTIADACLGAIAAGDIGK
CCCCCCCCCHHHHCCCCCEEEECEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
HFPDTDPAFKDADSAVLLQKVWGFVREQGYELGNLDCTIIAQKPKMAPHIESMRKRISEL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
LETSIDNINVKATTTEKLGFTGREEGIASQAVVLLQKK
HHHHHCCCEEEEECHHHCCCCCCCCCHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA