Click here to switch to the map view.

The map label for this gene is queF [H]

Identifier: 218230760

GI number: 218230760

Start: 1352686

End: 1353183

Strand: Direct

Name: queF [H]

Synonym: BCB4264_A1397

Alternate gene names: 218230760

Gene position: 1352686-1353183 (Clockwise)

Preceding gene: 218233066

Following gene: 218235273

Centisome position: 24.96

GC content: 33.13

Gene sequence:

>498_bases
ATGACAGGAAGATTAGATGAAGATTTAAAAGATGTAACATTACTAGGGAATCAAAATACAAAATATTTATTTGAATATAG
CCCAGAAATATTAGAGGTATTTGATAATAATCATCCAAACCGTGATTATTTTGTAAAATTCAATTGTCCTGAATTTACAA
GTTTATGCCCGAAAACAGGACAACCAGATTTTGCAACAATTTATATTAGCTATATTCCAGAGCAAAAAATGGTAGAGAGT
AAATCTTTAAAGTTGTATTTATTTAGTTTCCGCAATCATGGTGATTTCCATGAAGATTGCATGAACGTTATTATGAACGA
TTTAATTAAATTAATGGATCCACGTTATATTGAGGTATGGGGGAAATTCACACCACGTGGTGGTATTTCAATTGATCCAT
ACTGCAACTACGGGCGCCCAGGGACGAAGTATGAACAAATGGCTGATTACCGCATGATGAACCACGATCTATATCCGGAA
ACAATTGATAATCGTTAA

Upstream 100 bases:

>100_bases
TGTAAATTGTAAAGAGATGAATGATGCAAAAGTATTGCCTCAGCTTCATGCGTTAGTATGGGGAAATAAGCGCGGCGTAT
AACGAGAAGGGATGTTTAAA

Downstream 100 bases:

>100_bases
TAGAAAGAAAGGGCCTGTATAGTATACAGGCCCTTTCTTTCTATTATATTATGCATTTTGATTAATGACAGTATAGTTTT
TATGACTTACAACTGTAAGA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQKMVES
KSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPE
TIDNR

Sequences:

>Translated_165_residues
MTGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQKMVES
KSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPE
TIDNR
>Mature_164_residues
TGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTGQPDFATIYISYIPEQKMVESK
SLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVWGKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPET
IDNR

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=113, Percent_Identity=33.6283185840708, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 19530; Mature: 19399

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTG
CCCCCCCCCCCEEEECCCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCC
QPDFATIYISYIPEQKMVESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVW
CCCEEEEEEEECCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPETIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TGRLDEDLKDVTLLGNQNTKYLFEYSPEILEVFDNNHPNRDYFVKFNCPEFTSLCPKTG
CCCCCCCCCCEEEECCCCCEEEEEECHHHHHHHCCCCCCCCEEEEECCCHHHHCCCCCC
QPDFATIYISYIPEQKMVESKSLKLYLFSFRNHGDFHEDCMNVIMNDLIKLMDPRYIEVW
CCCEEEEEEEECCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEE
GKFTPRGGISIDPYCNYGRPGTKYEQMADYRMMNHDLYPETIDNR
ECCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA