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The map label for this gene is queE [H]

Identifier: 218233066

GI number: 218233066

Start: 1351951

End: 1352667

Strand: Direct

Name: queE [H]

Synonym: BCB4264_A1396

Alternate gene names: 218233066

Gene position: 1351951-1352667 (Clockwise)

Preceding gene: 218234630

Following gene: 218230760

Centisome position: 24.95

GC content: 35.56

Gene sequence:

>717_bases
ATGAGTAAAATCCCTGTCTTAGAAATATTCGGTCCGACTATTCAAGGTGAAGGAATGATTGTAGGGCAAAAGACAATGTT
TATCCGTACAGCTGGTTGTGATTATAGCTGTGCTTGGTGTGATTCTGCTTTTACGTGGGATGGATCAGCTAAAGATCAAA
TTAGACAGATGACACCAGAAGATATTTGGGATGAGCTTGTTGCAATTGGAGGAGAAAATTTTTCTCATGTTACGATTTCA
GGTGGAAATCCGGCATTACTGAAAAATATTGAGTTTCTTCTTTCTATATTAAAAGAAAATGGAATGCGAACGGCAATTGA
AACGCAAGGGAGTAAATGGCAAGATTGGTTACTTCAAATTGATGAGATAACAATTTCTCCAAAACCACCAAGTTCAACGA
TGAAAACAGATTTTCAGAGGTTAGATGCTATTATTCAGAAACTAGCAGGAAAAGATATTAGTTTAAAAGTAGTAGTATTC
GATGATCATGATTTTGAATATGCAGTTAAGATGCACGAACGTTATCCAAAGGTACCATTTTTCTTACAAGTAGGAAACGA
TGATACAAAAACAGTGGATGATGCGATGCTTATAAAAAAATTATTAGATAAGTATGAATGGCTGATTGATAAAGCTGTAA
ATTGTAAAGAGATGAATGATGCAAAAGTATTGCCTCAGCTTCATGCGTTAGTATGGGGAAATAAGCGCGGCGTATAA

Upstream 100 bases:

>100_bases
GAGAATCGAGTGAACGAATATAAAGGAGCTCGCGTTGAATTTGTTCGTCTATTTGAGACGCCGACTAGTTATGCGGAAGT
AAGACGGGAGTGGATGCTTG

Downstream 100 bases:

>100_bases
CGAGAAGGGATGTTTAAAATGACAGGAAGATTAGATGAAGATTTAAAAGATGTAACATTACTAGGGAATCAAAATACAAA
ATATTTATTTGAATATAGCC

Product: radical SAM domain protein

Products: NA

Alternate protein names: CDG synthase; Queuosine biosynthesis protein queE [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSKIPVLEIFGPTIQGEGMIVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPEDIWDELVAIGGENFSHVTIS
GGNPALLKNIEFLLSILKENGMRTAIETQGSKWQDWLLQIDEITISPKPPSSTMKTDFQRLDAIIQKLAGKDISLKVVVF
DDHDFEYAVKMHERYPKVPFFLQVGNDDTKTVDDAMLIKKLLDKYEWLIDKAVNCKEMNDAKVLPQLHALVWGNKRGV

Sequences:

>Translated_238_residues
MSKIPVLEIFGPTIQGEGMIVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPEDIWDELVAIGGENFSHVTIS
GGNPALLKNIEFLLSILKENGMRTAIETQGSKWQDWLLQIDEITISPKPPSSTMKTDFQRLDAIIQKLAGKDISLKVVVF
DDHDFEYAVKMHERYPKVPFFLQVGNDDTKTVDDAMLIKKLLDKYEWLIDKAVNCKEMNDAKVLPQLHALVWGNKRGV
>Mature_237_residues
SKIPVLEIFGPTIQGEGMIVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPEDIWDELVAIGGENFSHVTISG
GNPALLKNIEFLLSILKENGMRTAIETQGSKWQDWLLQIDEITISPKPPSSTMKTDFQRLDAIIQKLAGKDISLKVVVFD
DHDFEYAVKMHERYPKVPFFLQVGNDDTKTVDDAMLIKKLLDKYEWLIDKAVNCKEMNDAKVLPQLHALVWGNKRGV

Specific function: Catalyzes the conversion of 6-carboxy-5,6,7,8- tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG) [H]

COG id: COG0602

COG function: function code O; Organic radical activating enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. 7-carboxy-7- deazaguanine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1789139, Length=123, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017742
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 26886; Mature: 26755

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIPVLEIFGPTIQGEGMIVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPE
CCCCCEEEEECCEECCCEEEEECEEEEEEECCCCCEEEECCCCEECCCCCHHHHHHCCHH
DIWDELVAIGGENFSHVTISGGNPALLKNIEFLLSILKENGMRTAIETQGSKWQDWLLQI
HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHEEE
DEITISPKPPSSTMKTDFQRLDAIIQKLAGKDISLKVVVFDDHDFEYAVKMHERYPKVPF
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCE
FLQVGNDDTKTVDDAMLIKKLLDKYEWLIDKAVNCKEMNDAKVLPQLHALVWGNKRGV
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKIPVLEIFGPTIQGEGMIVGQKTMFIRTAGCDYSCAWCDSAFTWDGSAKDQIRQMTPE
CCCCEEEEECCEECCCEEEEECEEEEEEECCCCCEEEECCCCEECCCCCHHHHHHCCHH
DIWDELVAIGGENFSHVTISGGNPALLKNIEFLLSILKENGMRTAIETQGSKWQDWLLQI
HHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHEEE
DEITISPKPPSSTMKTDFQRLDAIIQKLAGKDISLKVVVFDDHDFEYAVKMHERYPKVPF
EEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHCCCCCE
FLQVGNDDTKTVDDAMLIKKLLDKYEWLIDKAVNCKEMNDAKVLPQLHALVWGNKRGV
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]