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The map label for this gene is cobB [H]

Identifier: 213693297

GI number: 213693297

Start: 2767303

End: 2768058

Strand: Direct

Name: cobB [H]

Synonym: Blon_2451

Alternate gene names: 213693297

Gene position: 2767303-2768058 (Clockwise)

Preceding gene: 213693295

Following gene: 213693300

Centisome position: 97.69

GC content: 56.75

Gene sequence:

>756_bases
ATGACCAAGAAGATTGCTGTATTAACCGGGGCGGGCATTTCCACGTCCGCCGGCATCCCTGATTTCCGCGGTCCGGACGG
TGTGTGGACCAAGCATCCTGACCAAATGAGCGTTTACGACATCGATCTGTTCCTGCGCAACAAAGAGGACCGCGAGTACT
CCTGGCGCTGGCAAAAGGAATCGCCAGTATGGAATGCGCAGCCCGGCACTGCGCATAAGGCGCTGGTCAAGCTGGAACAA
GCCGGCATGTTGACGCTGCTGGCCACGCAGAACTTCGATGCACTGCATGAGAAGGCCGGCAACAGCGACAACGTCATCGT
GAACCTGCACGGCACTATCGGCACCTCGCATTGCATGAAGTGCCATCAGGAATATGCCACGGCGGACATCATGGCCCGTT
TGGATGAAGAACCCGATCCACATTGCCATCGCAAACTGAAGTATCGTGGCGATATGCCATGCAATGGCATTATTAAAACC
GATGTGGTGTATTTCGGTGAGGCGCTGCCGGACGGAGCGATGGAGAAGTCGTACAGCCTGGCCACCAAAGCCGACGAACT
ATGGGTAATCGGATCGACCCTTGAGGTGTACCCGGCGGCGAGCATTGTGCCGGTGGCCGCGCAGGCGGGCGTGCCCATCA
CCATTATGAATATGGGTCACACGCAATATGACCATCTTGCGAGCCGCTTGATTCATGAGGATATCGCCGCTGCCCTGCCG
AAGTTGGTGGACGAGACGATTGCGGAGAACAAGTAG

Upstream 100 bases:

>100_bases
TGCGTTCATCTCTCGGCAACCACACTGCCGGCGCACAATCGGCACATGACAGCGCTCCGTCATCGGATTTCGTTCATGAA
TCGCGGGTATACCTGTGGTT

Downstream 100 bases:

>100_bases
CCCTCAGCATAACGAAAGCGCCGGTAGGTGGGTTAAGCAACCGATGATTGGCTTAACCACCCACCGGCGCTTTCGCGCCA
CCTCCCGCTAGCGGGAGGTA

Product: Silent information regulator protein Sir2

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog; Sir2Tm [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MTKKIAVLTGAGISTSAGIPDFRGPDGVWTKHPDQMSVYDIDLFLRNKEDREYSWRWQKESPVWNAQPGTAHKALVKLEQ
AGMLTLLATQNFDALHEKAGNSDNVIVNLHGTIGTSHCMKCHQEYATADIMARLDEEPDPHCHRKLKYRGDMPCNGIIKT
DVVYFGEALPDGAMEKSYSLATKADELWVIGSTLEVYPAASIVPVAAQAGVPITIMNMGHTQYDHLASRLIHEDIAAALP
KLVDETIAENK

Sequences:

>Translated_251_residues
MTKKIAVLTGAGISTSAGIPDFRGPDGVWTKHPDQMSVYDIDLFLRNKEDREYSWRWQKESPVWNAQPGTAHKALVKLEQ
AGMLTLLATQNFDALHEKAGNSDNVIVNLHGTIGTSHCMKCHQEYATADIMARLDEEPDPHCHRKLKYRGDMPCNGIIKT
DVVYFGEALPDGAMEKSYSLATKADELWVIGSTLEVYPAASIVPVAAQAGVPITIMNMGHTQYDHLASRLIHEDIAAALP
KLVDETIAENK
>Mature_250_residues
TKKIAVLTGAGISTSAGIPDFRGPDGVWTKHPDQMSVYDIDLFLRNKEDREYSWRWQKESPVWNAQPGTAHKALVKLEQA
GMLTLLATQNFDALHEKAGNSDNVIVNLHGTIGTSHCMKCHQEYATADIMARLDEEPDPHCHRKLKYRGDMPCNGIIKTD
VVYFGEALPDGAMEKSYSLATKADELWVIGSTLEVYPAASIVPVAAQAGVPITIMNMGHTQYDHLASRLIHEDIAAALPK
LVDETIAENK

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form (Probable). Also able to ADP- ribosylate peptide substrates with Arg or Lys in the +2 position. The role of this function in vivo is not clear [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=267, Percent_Identity=30.7116104868914, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI300797577, Length=242, Percent_Identity=27.6859504132231, Blast_Score=114, Evalue=6e-26,
Organism=Homo sapiens, GI7657575, Length=232, Percent_Identity=32.7586206896552, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI13775602, Length=223, Percent_Identity=33.1838565022422, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI6912660, Length=221, Percent_Identity=31.2217194570136, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI13775600, Length=223, Percent_Identity=33.1838565022422, Blast_Score=108, Evalue=3e-24,
Organism=Homo sapiens, GI63054862, Length=216, Percent_Identity=31.4814814814815, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI300797705, Length=202, Percent_Identity=33.1683168316832, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI6912664, Length=229, Percent_Identity=28.3842794759825, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI13787215, Length=229, Percent_Identity=28.3842794759825, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI300795542, Length=221, Percent_Identity=28.5067873303167, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI300797597, Length=235, Percent_Identity=26.8085106382979, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI7706712, Length=238, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI215982798, Length=190, Percent_Identity=28.9473684210526, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI308199517, Length=248, Percent_Identity=31.8548387096774, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17541892, Length=222, Percent_Identity=31.981981981982, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=263, Percent_Identity=30.7984790874525, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71990482, Length=264, Percent_Identity=29.5454545454545, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17505510, Length=242, Percent_Identity=26.0330578512397, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6325242, Length=223, Percent_Identity=31.8385650224215, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324504, Length=251, Percent_Identity=30.6772908366534, Blast_Score=99, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6320163, Length=283, Percent_Identity=29.3286219081272, Blast_Score=96, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324599, Length=286, Percent_Identity=26.9230769230769, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648389, Length=207, Percent_Identity=33.8164251207729, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17137536, Length=249, Percent_Identity=32.9317269076305, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24645650, Length=244, Percent_Identity=27.8688524590164, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571445, Length=263, Percent_Identity=29.277566539924, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28571443, Length=224, Percent_Identity=27.6785714285714, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI28571441, Length=224, Percent_Identity=27.6785714285714, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24650933, Length=241, Percent_Identity=26.5560165975104, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27681; Mature: 27550

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIAVLTGAGISTSAGIPDFRGPDGVWTKHPDQMSVYDIDLFLRNKEDREYSWRWQKE
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCC
SPVWNAQPGTAHKALVKLEQAGMLTLLATQNFDALHEKAGNSDNVIVNLHGTIGTSHCMK
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEEEECCCCHHHHHH
CHQEYATADIMARLDEEPDPHCHRKLKYRGDMPCNGIIKTDVVYFGEALPDGAMEKSYSL
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEHHHEECCCCCCCCHHHHCHHH
ATKADELWVIGSTLEVYPAASIVPVAAQAGVPITIMNMGHTQYDHLASRLIHEDIAAALP
HCCCCCEEEECCCEEECCCCCEEEEHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
KLVDETIAENK
HHHHHHHHCCC
>Mature Secondary Structure 
TKKIAVLTGAGISTSAGIPDFRGPDGVWTKHPDQMSVYDIDLFLRNKEDREYSWRWQKE
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCC
SPVWNAQPGTAHKALVKLEQAGMLTLLATQNFDALHEKAGNSDNVIVNLHGTIGTSHCMK
CCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEEEECCCCHHHHHH
CHQEYATADIMARLDEEPDPHCHRKLKYRGDMPCNGIIKTDVVYFGEALPDGAMEKSYSL
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEHHHEECCCCCCCCHHHHCHHH
ATKADELWVIGSTLEVYPAASIVPVAAQAGVPITIMNMGHTQYDHLASRLIHEDIAAALP
HCCCCCEEEECCCEEECCCCCEEEEHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
KLVDETIAENK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]