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The map label for this gene is thyA [H]

Identifier: 213692995

GI number: 213692995

Start: 2367828

End: 2368700

Strand: Reverse

Name: thyA [H]

Synonym: Blon_2142

Alternate gene names: 213692995

Gene position: 2368700-2367828 (Counterclockwise)

Preceding gene: 213692997

Following gene: 213692994

Centisome position: 83.62

GC content: 58.99

Gene sequence:

>873_bases
ATGGCTTTGACCGAAGAACAACTCGCAGATATTCGCTCCCGTATCCCCGCACGTCCCGAAACGGACATTCCGATGCCCTA
TGAGGACCTGGTACGCAAGATCCTGACCGAAGGCACGCTGAAGTCCGATCGCACCGGCACCGGCACGATTTCCCTGTTCG
GCCAGCAGATGCGCTTTGACCTGTCGAAGTACTTCCCGTTGCTGACCACCAAGACCGTGTTCTTCAAAGGCCTGGCCTAT
GAGCTGCTGTGGTTTTTGAAGGGTTCCTCCAACATCAACTGGCTGCTGGAGCACAACGTGCATATTTGGGATGAGTGGGC
GGACGAGAACGGCGATCTGGGCCCGGTCTATGGCGTGCAGTGGCGTTCCTGGCCCGCGCCGACTCCCGAGGACCCGAATC
GCACCATCGACCAGATCTTCAATGTGCTCGACCTCATCAAGCATCATCCGGATTCGCGCCGCATGATCGTCTCCGCGTGG
AATCCGGCCGAGGTGGAGAAGATGGCGTTGCCGCCCTGCCACGCGCTCTTCCAGTTCTACGTGGCCGACGGCAAGCTAAG
CTGCCAGCTGTACCAGCGTTCCTGTGACATGTTCCTCGGCGTGCCATTCAACATCGCCTCCTACTCGCTGCTCACCATGA
TGATGGCGCAGCAGACCGGGCTCAAGCCGGGCGAATTCGTCTGGACCGGCGGCGACTGCCATGTTTACGACAACCACGTC
GACCAGTTCCTTGAGCAGCTGGGCCGCAAGCCCTACCCGTACCCGACCATCGAGATCCGTAAGGCCGATTCTCTGTTCGA
CTACCAGTATGAGGACTTCAAGGTCGTCGGCTACCAGCATCACCCTACTATTAAGGCACCTGTTGCGGTCTGA

Upstream 100 bases:

>100_bases
CGATCTGGCCCATCGCCATGCCAGCGGGGGAGCGCGGCACCTGGCCGCTCTCCCGCATCATTGGACTTCTCTATTATGCC
CCTAAGCTACACTGGTGCAC

Downstream 100 bases:

>100_bases
TGCTGATTACCATTTCGTGGACAGCGAAGACTGTGAAGGTGACGGACCCCGAGTCTTCGCTGTCCACTACACTAAGGCCC
AGTTCCGCAATCCAGGAGGA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MALTEEQLADIRSRIPARPETDIPMPYEDLVRKILTEGTLKSDRTGTGTISLFGQQMRFDLSKYFPLLTTKTVFFKGLAY
ELLWFLKGSSNINWLLEHNVHIWDEWADENGDLGPVYGVQWRSWPAPTPEDPNRTIDQIFNVLDLIKHHPDSRRMIVSAW
NPAEVEKMALPPCHALFQFYVADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQTGLKPGEFVWTGGDCHVYDNHV
DQFLEQLGRKPYPYPTIEIRKADSLFDYQYEDFKVVGYQHHPTIKAPVAV

Sequences:

>Translated_290_residues
MALTEEQLADIRSRIPARPETDIPMPYEDLVRKILTEGTLKSDRTGTGTISLFGQQMRFDLSKYFPLLTTKTVFFKGLAY
ELLWFLKGSSNINWLLEHNVHIWDEWADENGDLGPVYGVQWRSWPAPTPEDPNRTIDQIFNVLDLIKHHPDSRRMIVSAW
NPAEVEKMALPPCHALFQFYVADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQTGLKPGEFVWTGGDCHVYDNHV
DQFLEQLGRKPYPYPTIEIRKADSLFDYQYEDFKVVGYQHHPTIKAPVAV
>Mature_289_residues
ALTEEQLADIRSRIPARPETDIPMPYEDLVRKILTEGTLKSDRTGTGTISLFGQQMRFDLSKYFPLLTTKTVFFKGLAYE
LLWFLKGSSNINWLLEHNVHIWDEWADENGDLGPVYGVQWRSWPAPTPEDPNRTIDQIFNVLDLIKHHPDSRRMIVSAWN
PAEVEKMALPPCHALFQFYVADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQTGLKPGEFVWTGGDCHVYDNHVD
QFLEQLGRKPYPYPTIEIRKADSLFDYQYEDFKVVGYQHHPTIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=283, Percent_Identity=51.5901060070671, Blast_Score=282, Evalue=2e-76,
Organism=Escherichia coli, GI1789191, Length=265, Percent_Identity=64.5283018867924, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=49.645390070922, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=40.3448275862069, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17137556, Length=285, Percent_Identity=53.3333333333333, Blast_Score=291, Evalue=3e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 33427; Mature: 33295

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTEEQLADIRSRIPARPETDIPMPYEDLVRKILTEGTLKSDRTGTGTISLFGQQMRFD
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHH
LSKYFPLLTTKTVFFKGLAYELLWFLKGSSNINWLLEHNVHIWDEWADENGDLGPVYGVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCCCCCCEECCC
WRSWPAPTPEDPNRTIDQIFNVLDLIKHHPDSRRMIVSAWNPAEVEKMALPPCHALFQFY
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHH
VADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQTGLKPGEFVWTGGDCHVYDNHV
HCCCCEEEHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEHHHH
DQFLEQLGRKPYPYPTIEIRKADSLFDYQYEDFKVVGYQHHPTIKAPVAV
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
ALTEEQLADIRSRIPARPETDIPMPYEDLVRKILTEGTLKSDRTGTGTISLFGQQMRFD
CCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHH
LSKYFPLLTTKTVFFKGLAYELLWFLKGSSNINWLLEHNVHIWDEWADENGDLGPVYGVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCCCCCCCEECCC
WRSWPAPTPEDPNRTIDQIFNVLDLIKHHPDSRRMIVSAWNPAEVEKMALPPCHALFQFY
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHH
VADGKLSCQLYQRSCDMFLGVPFNIASYSLLTMMMAQQTGLKPGEFVWTGGDCHVYDNHV
HCCCCEEEHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEHHHH
DQFLEQLGRKPYPYPTIEIRKADSLFDYQYEDFKVVGYQHHPTIKAPVAV
HHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]