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The map label for this gene is dhfrIII [H]

Identifier: 213692994

GI number: 213692994

Start: 2367056

End: 2367610

Strand: Reverse

Name: dhfrIII [H]

Synonym: Blon_2141

Alternate gene names: 213692994

Gene position: 2367610-2367056 (Counterclockwise)

Preceding gene: 213692995

Following gene: 213692993

Centisome position: 83.58

GC content: 61.44

Gene sequence:

>555_bases
GTGAATCTTATCTGGGCCGAAGCCCGCGACAAGGAAGGTCGCGCCGGTGCCATCGGCCTCAATGGCGGCATGCCTTGGCA
CTGTGCCGAGGATATGAAGCATTTCAAGGAGCTGACCGTCTCCCATCCGGTGATTATGGGTCGTAAGACTTGGGAATCGC
TGGGGGTCAAGTACCGGCCGCTGCCCAACCGCGACAACATCGTCGTTTCCCACGATCCGATGTACCGTGCGCCGGGGGCC
ACTGTGGTCACCAGTCTTGACGACGCGCTCGATATGGCCCGTCAGGAGGCGATTCCGGACGACGGTCTGGACCGTTCGGA
GATTTGGATCATCGGCGGTGCCCAGCTGTTCGCCGAGGCTTTGCCGTTCGCGGACAAAGCCTATGTGACTGATCTGGCCG
CCACCGTGGATGCCGACAGCTACGCGCCCGATATGGCGTCGCTGGTCGAGGCCGGGATGTGGCGGGAAGCCGAAGTGGGC
GAGTGGCATACGCCCGCCAAGGAGGAGTCCGGCATCGATTCCTATCGTTTCCGTATCTTGGCTAAAACCAAGTGA

Upstream 100 bases:

>100_bases
TGACAGTAGCCGTAGCGGCTATCACGAACCCGAGCCCGGATCTGCCGGGCTGTCTGCCACTGAAGAAGACTGGGGTGACG
ATTATCCCAAGACCTTCTCG

Downstream 100 bases:

>100_bases
GCATGCGAATCCGGCTTATGCCCTTTATAGCGGCATGAGCCGGATTCATATTCATTCGACCCCAACAATACAGTCGGGCC
GTGTGCGGCCCGTTGAAGTC

Product: Dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNLIWAEARDKEGRAGAIGLNGGMPWHCAEDMKHFKELTVSHPVIMGRKTWESLGVKYRPLPNRDNIVVSHDPMYRAPGA
TVVTSLDDALDMARQEAIPDDGLDRSEIWIIGGAQLFAEALPFADKAYVTDLAATVDADSYAPDMASLVEAGMWREAEVG
EWHTPAKEESGIDSYRFRILAKTK

Sequences:

>Translated_184_residues
MNLIWAEARDKEGRAGAIGLNGGMPWHCAEDMKHFKELTVSHPVIMGRKTWESLGVKYRPLPNRDNIVVSHDPMYRAPGA
TVVTSLDDALDMARQEAIPDDGLDRSEIWIIGGAQLFAEALPFADKAYVTDLAATVDADSYAPDMASLVEAGMWREAEVG
EWHTPAKEESGIDSYRFRILAKTK
>Mature_184_residues
MNLIWAEARDKEGRAGAIGLNGGMPWHCAEDMKHFKELTVSHPVIMGRKTWESLGVKYRPLPNRDNIVVSHDPMYRAPGA
TVVTSLDDALDMARQEAIPDDGLDRSEIWIIGGAQLFAEALPFADKAYVTDLAATVDADSYAPDMASLVEAGMWREAEVG
EWHTPAKEESGIDSYRFRILAKTK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=181, Percent_Identity=30.3867403314917, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1786233, Length=182, Percent_Identity=36.8131868131868, Blast_Score=92, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505899, Length=182, Percent_Identity=32.967032967033, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=169, Percent_Identity=29.585798816568, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI116008026, Length=178, Percent_Identity=28.6516853932584, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24647458, Length=178, Percent_Identity=28.6516853932584, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20373; Mature: 20373

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLIWAEARDKEGRAGAIGLNGGMPWHCAEDMKHFKELTVSHPVIMGRKTWESLGVKYRP
CCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHCCEEECC
LPNRDNIVVSHDPMYRAPGATVVTSLDDALDMARQEAIPDDGLDRSEIWIIGGAQLFAEA
CCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHH
LPFADKAYVTDLAATVDADSYAPDMASLVEAGMWREAEVGEWHTPAKEESGIDSYRFRIL
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
AKTK
EECC
>Mature Secondary Structure
MNLIWAEARDKEGRAGAIGLNGGMPWHCAEDMKHFKELTVSHPVIMGRKTWESLGVKYRP
CCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCHHHCCEEECC
LPNRDNIVVSHDPMYRAPGATVVTSLDDALDMARQEAIPDDGLDRSEIWIIGGAQLFAEA
CCCCCCEEEECCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHH
LPFADKAYVTDLAATVDADSYAPDMASLVEAGMWREAEVGEWHTPAKEESGIDSYRFRIL
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
AKTK
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]