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The map label for this gene is lacE [H]

Identifier: 213692874

GI number: 213692874

Start: 2199973

End: 2201394

Strand: Reverse

Name: lacE [H]

Synonym: Blon_2015

Alternate gene names: 213692874

Gene position: 2201394-2199973 (Counterclockwise)

Preceding gene: 213692875

Following gene: 213692871

Centisome position: 77.71

GC content: 57.67

Gene sequence:

>1422_bases
ATGCGAGTGATCGGACAGCGAATCAAAAGAATGGCAACCATAGCAGTGACTGCCATCCTTTCCGTATCCCTGGCCTCGTG
CGGGCAGGCTACGGACGACAACCGTACGGAGATCAGCGTATGGAGCTGGGAACCGAGCATGGGAGAGGTCGTCCGCCGCT
TCGAAAAAGCCAATCCGGACATCCGCGTGAAATGGACCAACATCTCCGGCTACGACAATCTCAATACCGCCATTCAGGAC
GGTTACGGCACGCCGGACGTGGCTCAAATCGAATACTATGCACTGTTGCAGTACGCGGTCTCCGGCCAATTGCTTGATCT
GACCGACAAGATCGGCTCGGATTATTCCAACTTCTTTACCCTCGGCACATGGTCGTCCGTGCAGCTCGCCGGCCATATCT
ACGGGCTGCCCATGGATTCGGGCCCGATGGGTTTCTTCTACAACGAAGACGTGTTCCGCCAAGCCGGCGTGGACGCCACC
AAGATCAAGACTTGGGATGACTACTATGAGGCCGCCAAAAAGCTCAAGGAGATCGGCGTGTACATCGCCGCCGACTCGGG
TGATGGCAGTTTCTATGACGCGATGGTCTGGCTTGCCGGTGGTCAGCCGTTCCACACGTCGGCCGACGGCAAAACCGTGA
CCATCGATTTGGATGAGGATGCCGGCACCCAACGGTTCACCGAATTCTGGCAGAAAATGATCGATGAGGGGCTTGTCGAT
ACCACCTCCGCCACATGGTCGGATCGGTGGAAAAGCAGCGTGAGCACCGGCACCATCGCCTCCGTGTTCTCCGGAGCTTG
GATGCCGTCGCTGCTGCTGTCCAATGTGCCGGGCGCGGCCGGTCTGTGGCGCGTGGCTCCCATGCCCACCTATGGTGGTC
AGCCCGCCAATGCCGAAAGCGGCGGATCGGCGCTGACCGTGTTGCAGCTGACCCGTAAGCCGGATGCGGCCTATCGTTTC
GTGGACTTTGTGGCGCATAACGCCCAAGGCATTAGCGCGCGTGTGGATGGCGGTGCTTTCCCGGCCGATTATGACACCCT
GAATTCCGCTGATTTTTTGGATAAAACCACGATTACCAATGACCGTGGCATTGAAATCCCGTATTTCGGCGGGCAGAAGT
TCAACCGTGTACTGTCGGAGGCCGCCGAGGATGTGCCGATCGGATACCAGTATCTGCCGTTCGAAGTGTATGCGCGCAGT
GACTTCAAATCTACGGTGGGCCAGGCATACGAATGGTCCAGCAGTTTCCACGACTATCAGCAGCGACAGGAAGCCATTGA
GATGGGCATGAAGGACGAGGAAGGCAACCCGCTCGAACCGTTGGAGAAGCCCGGCAAGAAAGTCAGTCTCTCCGACGGCC
TGGCCCAATGGCAGAAAGACCTCCGAGAATACGGCTTCAATCAGGGCTTTACGATTAAGTAG

Upstream 100 bases:

>100_bases
CGTGAAGGCCTCGAGCGCTTCGGCGGAATGCTGGCTGTTGTGAGTGGTCATGAGCAGGATGCATCTACCGTTTTAAGCCC
CGCCTAGTACACTCGTCACC

Downstream 100 bases:

>100_bases
GGTTTTGAGTTGGTTTTCGGGGAGGGACGGAATCGACTGCGCTATGCGCGGCCGATTCCTGCACGCCGCGTTACTTCACA
TTGCGCGAGTAGATGTATGC

Product: extracellular solute-binding protein, family 1

Products: NA

Alternate protein names: LBP [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MRVIGQRIKRMATIAVTAILSVSLASCGQATDDNRTEISVWSWEPSMGEVVRRFEKANPDIRVKWTNISGYDNLNTAIQD
GYGTPDVAQIEYYALLQYAVSGQLLDLTDKIGSDYSNFFTLGTWSSVQLAGHIYGLPMDSGPMGFFYNEDVFRQAGVDAT
KIKTWDDYYEAAKKLKEIGVYIAADSGDGSFYDAMVWLAGGQPFHTSADGKTVTIDLDEDAGTQRFTEFWQKMIDEGLVD
TTSATWSDRWKSSVSTGTIASVFSGAWMPSLLLSNVPGAAGLWRVAPMPTYGGQPANAESGGSALTVLQLTRKPDAAYRF
VDFVAHNAQGISARVDGGAFPADYDTLNSADFLDKTTITNDRGIEIPYFGGQKFNRVLSEAAEDVPIGYQYLPFEVYARS
DFKSTVGQAYEWSSSFHDYQQRQEAIEMGMKDEEGNPLEPLEKPGKKVSLSDGLAQWQKDLREYGFNQGFTIK

Sequences:

>Translated_473_residues
MRVIGQRIKRMATIAVTAILSVSLASCGQATDDNRTEISVWSWEPSMGEVVRRFEKANPDIRVKWTNISGYDNLNTAIQD
GYGTPDVAQIEYYALLQYAVSGQLLDLTDKIGSDYSNFFTLGTWSSVQLAGHIYGLPMDSGPMGFFYNEDVFRQAGVDAT
KIKTWDDYYEAAKKLKEIGVYIAADSGDGSFYDAMVWLAGGQPFHTSADGKTVTIDLDEDAGTQRFTEFWQKMIDEGLVD
TTSATWSDRWKSSVSTGTIASVFSGAWMPSLLLSNVPGAAGLWRVAPMPTYGGQPANAESGGSALTVLQLTRKPDAAYRF
VDFVAHNAQGISARVDGGAFPADYDTLNSADFLDKTTITNDRGIEIPYFGGQKFNRVLSEAAEDVPIGYQYLPFEVYARS
DFKSTVGQAYEWSSSFHDYQQRQEAIEMGMKDEEGNPLEPLEKPGKKVSLSDGLAQWQKDLREYGFNQGFTIK
>Mature_473_residues
MRVIGQRIKRMATIAVTAILSVSLASCGQATDDNRTEISVWSWEPSMGEVVRRFEKANPDIRVKWTNISGYDNLNTAIQD
GYGTPDVAQIEYYALLQYAVSGQLLDLTDKIGSDYSNFFTLGTWSSVQLAGHIYGLPMDSGPMGFFYNEDVFRQAGVDAT
KIKTWDDYYEAAKKLKEIGVYIAADSGDGSFYDAMVWLAGGQPFHTSADGKTVTIDLDEDAGTQRFTEFWQKMIDEGLVD
TTSATWSDRWKSSVSTGTIASVFSGAWMPSLLLSNVPGAAGLWRVAPMPTYGGQPANAESGGSALTVLQLTRKPDAAYRF
VDFVAHNAQGISARVDGGAFPADYDTLNSADFLDKTTITNDRGIEIPYFGGQKFNRVLSEAAEDVPIGYQYLPFEVYARS
DFKSTVGQAYEWSSSFHDYQQRQEAIEMGMKDEEGNPLEPLEKPGKKVSLSDGLAQWQKDLREYGFNQGFTIK

Specific function: Part of the binding-protein-dependent transport system for lactose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 52218; Mature: 52218

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVIGQRIKRMATIAVTAILSVSLASCGQATDDNRTEISVWSWEPSMGEVVRRFEKANPD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
IRVKWTNISGYDNLNTAIQDGYGTPDVAQIEYYALLQYAVSGQLLDLTDKIGSDYSNFFT
EEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHCCCCHHEEE
LGTWSSVQLAGHIYGLPMDSGPMGFFYNEDVFRQAGVDATKIKTWDDYYEAAKKLKEIGV
ECCCCCEEEEEEEEECCCCCCCCCEEECHHHHHHCCCCHHEECCHHHHHHHHHHHHHCCE
YIAADSGDGSFYDAMVWLAGGQPFHTSADGKTVTIDLDEDAGTQRFTEFWQKMIDEGLVD
EEEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
TTSATWSDRWKSSVSTGTIASVFSGAWMPSLLLSNVPGAAGLWRVAPMPTYGGQPANAES
CCCCCHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCC
GGSALTVLQLTRKPDAAYRFVDFVAHNAQGISARVDGGAFPADYDTLNSADFLDKTTITN
CCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHCCCCCCCC
DRGIEIPYFGGQKFNRVLSEAAEDVPIGYQYLPFEVYARSDFKSTVGQAYEWSSSFHDYQ
CCCCEECCCCCHHHHHHHHHHHHCCCCCEEECCEEEEECCHHHHHHCCHHHHHHHHHHHH
QRQEAIEMGMKDEEGNPLEPLEKPGKKVSLSDGLAQWQKDLREYGFNQGFTIK
HHHHHHHCCCCCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRVIGQRIKRMATIAVTAILSVSLASCGQATDDNRTEISVWSWEPSMGEVVRRFEKANPD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCC
IRVKWTNISGYDNLNTAIQDGYGTPDVAQIEYYALLQYAVSGQLLDLTDKIGSDYSNFFT
EEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHCCCCHHEEE
LGTWSSVQLAGHIYGLPMDSGPMGFFYNEDVFRQAGVDATKIKTWDDYYEAAKKLKEIGV
ECCCCCEEEEEEEEECCCCCCCCCEEECHHHHHHCCCCHHEECCHHHHHHHHHHHHHCCE
YIAADSGDGSFYDAMVWLAGGQPFHTSADGKTVTIDLDEDAGTQRFTEFWQKMIDEGLVD
EEEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
TTSATWSDRWKSSVSTGTIASVFSGAWMPSLLLSNVPGAAGLWRVAPMPTYGGQPANAES
CCCCCHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCC
GGSALTVLQLTRKPDAAYRFVDFVAHNAQGISARVDGGAFPADYDTLNSADFLDKTTITN
CCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCHHCCCCCCCC
DRGIEIPYFGGQKFNRVLSEAAEDVPIGYQYLPFEVYARSDFKSTVGQAYEWSSSFHDYQ
CCCCEECCCCCHHHHHHHHHHHHCCCCCEEECCEEEEECCHHHHHHCCHHHHHHHHHHHH
QRQEAIEMGMKDEEGNPLEPLEKPGKKVSLSDGLAQWQKDLREYGFNQGFTIK
HHHHHHHCCCCCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630315; 2318800 [H]