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The map label for this gene is gsiB [H]

Identifier: 213692871

GI number: 213692871

Start: 2195896

End: 2197560

Strand: Reverse

Name: gsiB [H]

Synonym: Blon_2012

Alternate gene names: 213692871

Gene position: 2197560-2195896 (Counterclockwise)

Preceding gene: 213692874

Following gene: 213692870

Centisome position: 77.58

GC content: 62.76

Gene sequence:

>1665_bases
ATGTCCCAGAACAATGGTGTGCACGGCGAGTCCGGCGCAACCGAAGACAGGAACATCGACGGCGCGAATCAAGCCGCCAG
CACCGACGCGAGCCTGAACGCGCGCGCGAAATCATCAAACAAGAAGAACGGCGGCCCGAAATGGTGGCTGTGGACGCTGA
TCGCGGTCGTCGTGGCCGCGGCGCTCATCGTGGGCGTCACCGTCTTCGGCGGGAAAGGCAAGGGCAAGGACGCGAACGCC
TCCGGCTCGGCCAGCCGATCCGACACCGTGGCCGTCGGCCTCAAACTCGCCCCGGTCAATCTTGACATCCGCCACCAGTC
GGGTTCGGCCCTTGAGCAGATCCTCATCGGCAACGTGTACGAGGGTCTCGTCTCGCGTGGCTCGGACAACAAGGTCCACC
CCGGTCTGGCCAAGAGCTGGGAGATCTCCAAGGATGGCCTGACCTACACATTCCACCTCAACGAGAAGATGAACTTCTCC
AACGGCGACAAGCTCGACGCCGAGGATGTGGCGTGGTCCATCAACGAGCTCAAGGCCCAGAAGTACTACGACTACGAGCA
GGTCAAGAGCCTGGACAAGGCCGAGGCCGTGGACGCCAACACGGTCAGGCTCACCCTCTCCGCGCCCAACTCGAACATGC
TGTGGTACCTCACCAGCCGTCCCGGCCTGGTCTACGACAAGGACGCCACCTGGGATCAGAAGAACGGCGCGTTGGGCTCG
GGCCCGTACACCGTGGTCTCCTTTGACCCGAACGACAAGATCGTGCTCAAGGCGAACCCCAAGTATTGGGGCACCGAGCA
CAAGGCGAGGACCGAGAACGTGATCGTCCGGTTCCTGCCGGACGAGAACGCCAGCATCAACGCCCTCAAGAGCGGCGACG
TGCAGGTGCTTTCGCCCATCTCCGCCACCCTTGCCGATCCGTTCCGCAAGGATCCGAACTATGTGGTCAAGTCCGGTGAC
GGTTCCGACAAGTTCGTGCTCGCCTTCAACATGGCCAACGACAAGCTCTCCGACAAGCGCGTCCGTCAGGCCATCCGATA
CGCCATCGACCATAAGCAGATCATCGCCTCGCGCGGCGGCGTGGACGCCCCCCTCGGCGGTCCGATCCCCAAGGTCGATC
CCGGTTACGAGGATCTGAGCGGCCTGTACCCGCATGACGTGGCCAAGGCCAAGAAGCTCATGGAGCAGGCCGGATACTCC
GAGTCGAAGCCGCTGGACCTGACCCTCACCTACGCCAACGTGTACGGCACCGAACTCGGCGACCAGCTCAAGAGCCAGCT
CAAGCCCATCGGCATCGACCTGAAGATCAACTACGTGGAGTTCTCCACTTGGCTGCAGGACGTGCACGCCAACGGCAACT
ACGATCTTTCGCTCGTGGACCATGCCGAAAGCCACGACTTCTACGGATGGACCACGCCGAGCTACTACTTCCACTACGAC
AACAAGGACGTGCAGGCCCTGTACGCCAAGGCGCTGGAGGCCACCTCCGACGACGAGGCCTCGAAGTATCTGGCCAAGGC
CGCGAAGATCGTCTCCGAGGACGCGCCGGCCGACTGGCTGTTCAGCTGGAAGGTCACCATCGCCTACGCCAAGGGCGTGG
ACGGCTTCCCGCAGAACCTGAGCCAGACCGTGCTGCCCCTCTGGCGGATCACCTACACCAAGTAG

Upstream 100 bases:

>100_bases
GTAGAACGTGGTGATCGACCCGAATGGGCGGTCAACCACGCAAGATAACAACGAAACCATATTCGGGCGTAAGCATGCCC
GAATGCATCAGGGAGTATCC

Downstream 100 bases:

>100_bases
TCCCGGAACCCATCGGTCGGGCGCGGCGCGGGAGTGATTCCGTGGCGCGCCCGACCGTGGACGGCCCATCCGGCCGGTGA
CGCCACGGGCGGTGGCGAGG

Product: extracellular solute-binding protein, family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSQNNGVHGESGATEDRNIDGANQAASTDASLNARAKSSNKKNGGPKWWLWTLIAVVVAAALIVGVTVFGGKGKGKDANA
SGSASRSDTVAVGLKLAPVNLDIRHQSGSALEQILIGNVYEGLVSRGSDNKVHPGLAKSWEISKDGLTYTFHLNEKMNFS
NGDKLDAEDVAWSINELKAQKYYDYEQVKSLDKAEAVDANTVRLTLSAPNSNMLWYLTSRPGLVYDKDATWDQKNGALGS
GPYTVVSFDPNDKIVLKANPKYWGTEHKARTENVIVRFLPDENASINALKSGDVQVLSPISATLADPFRKDPNYVVKSGD
GSDKFVLAFNMANDKLSDKRVRQAIRYAIDHKQIIASRGGVDAPLGGPIPKVDPGYEDLSGLYPHDVAKAKKLMEQAGYS
ESKPLDLTLTYANVYGTELGDQLKSQLKPIGIDLKINYVEFSTWLQDVHANGNYDLSLVDHAESHDFYGWTTPSYYFHYD
NKDVQALYAKALEATSDDEASKYLAKAAKIVSEDAPADWLFSWKVTIAYAKGVDGFPQNLSQTVLPLWRITYTK

Sequences:

>Translated_554_residues
MSQNNGVHGESGATEDRNIDGANQAASTDASLNARAKSSNKKNGGPKWWLWTLIAVVVAAALIVGVTVFGGKGKGKDANA
SGSASRSDTVAVGLKLAPVNLDIRHQSGSALEQILIGNVYEGLVSRGSDNKVHPGLAKSWEISKDGLTYTFHLNEKMNFS
NGDKLDAEDVAWSINELKAQKYYDYEQVKSLDKAEAVDANTVRLTLSAPNSNMLWYLTSRPGLVYDKDATWDQKNGALGS
GPYTVVSFDPNDKIVLKANPKYWGTEHKARTENVIVRFLPDENASINALKSGDVQVLSPISATLADPFRKDPNYVVKSGD
GSDKFVLAFNMANDKLSDKRVRQAIRYAIDHKQIIASRGGVDAPLGGPIPKVDPGYEDLSGLYPHDVAKAKKLMEQAGYS
ESKPLDLTLTYANVYGTELGDQLKSQLKPIGIDLKINYVEFSTWLQDVHANGNYDLSLVDHAESHDFYGWTTPSYYFHYD
NKDVQALYAKALEATSDDEASKYLAKAAKIVSEDAPADWLFSWKVTIAYAKGVDGFPQNLSQTVLPLWRITYTK
>Mature_553_residues
SQNNGVHGESGATEDRNIDGANQAASTDASLNARAKSSNKKNGGPKWWLWTLIAVVVAAALIVGVTVFGGKGKGKDANAS
GSASRSDTVAVGLKLAPVNLDIRHQSGSALEQILIGNVYEGLVSRGSDNKVHPGLAKSWEISKDGLTYTFHLNEKMNFSN
GDKLDAEDVAWSINELKAQKYYDYEQVKSLDKAEAVDANTVRLTLSAPNSNMLWYLTSRPGLVYDKDATWDQKNGALGSG
PYTVVSFDPNDKIVLKANPKYWGTEHKARTENVIVRFLPDENASINALKSGDVQVLSPISATLADPFRKDPNYVVKSGDG
SDKFVLAFNMANDKLSDKRVRQAIRYAIDHKQIIASRGGVDAPLGGPIPKVDPGYEDLSGLYPHDVAKAKKLMEQAGYSE
SKPLDLTLTYANVYGTELGDQLKSQLKPIGIDLKINYVEFSTWLQDVHANGNYDLSLVDHAESHDFYGWTTPSYYFHYDN
KDVQALYAKALEATSDDEASKYLAKAAKIVSEDAPADWLFSWKVTIAYAKGVDGFPQNLSQTVLPLWRITYTK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=477, Percent_Identity=23.2704402515723, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789966, Length=503, Percent_Identity=25.4473161033797, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787762, Length=447, Percent_Identity=26.3982102908277, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081878, Length=491, Percent_Identity=24.2362525458248, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1789887, Length=351, Percent_Identity=26.2108262108262, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1787495, Length=521, Percent_Identity=23.9923224568138, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1789397, Length=489, Percent_Identity=24.1308793456033, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787551, Length=302, Percent_Identity=24.1721854304636, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60630; Mature: 60499

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNNGVHGESGATEDRNIDGANQAASTDASLNARAKSSNKKNGGPKWWLWTLIAVVVAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALIVGVTVFGGKGKGKDANASGSASRSDTVAVGLKLAPVNLDIRHQSGSALEQILIGNVY
HHHHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHH
EGLVSRGSDNKVHPGLAKSWEISKDGLTYTFHLNEKMNFSNGDKLDAEDVAWSINELKAQ
HHHHHCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KYYDYEQVKSLDKAEAVDANTVRLTLSAPNSNMLWYLTSRPGLVYDKDATWDQKNGALGS
HCCCHHHHHCCHHHHCCCCCEEEEEEECCCCCEEEEEECCCCEEECCCCCCCCCCCCCCC
GPYTVVSFDPNDKIVLKANPKYWGTEHKARTENVIVRFLPDENASINALKSGDVQVLSPI
CCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEECCH
SATLADPFRKDPNYVVKSGDGSDKFVLAFNMANDKLSDKRVRQAIRYAIDHKQIIASRGG
HHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VDAPLGGPIPKVDPGYEDLSGLYPHDVAKAKKLMEQAGYSESKPLDLTLTYANVYGTELG
CCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHH
DQLKSQLKPIGIDLKINYVEFSTWLQDVHANGNYDLSLVDHAESHDFYGWTTPSYYFHYD
HHHHHHCCCEEEEEEEEEEEHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEC
NKDVQALYAKALEATSDDEASKYLAKAAKIVSEDAPADWLFSWKVTIAYAKGVDGFPQNL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCHHH
SQTVLPLWRITYTK
HHHHHHHEEEEECC
>Mature Secondary Structure 
SQNNGVHGESGATEDRNIDGANQAASTDASLNARAKSSNKKNGGPKWWLWTLIAVVVAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALIVGVTVFGGKGKGKDANASGSASRSDTVAVGLKLAPVNLDIRHQSGSALEQILIGNVY
HHHHHEEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHH
EGLVSRGSDNKVHPGLAKSWEISKDGLTYTFHLNEKMNFSNGDKLDAEDVAWSINELKAQ
HHHHHCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KYYDYEQVKSLDKAEAVDANTVRLTLSAPNSNMLWYLTSRPGLVYDKDATWDQKNGALGS
HCCCHHHHHCCHHHHCCCCCEEEEEEECCCCCEEEEEECCCCEEECCCCCCCCCCCCCCC
GPYTVVSFDPNDKIVLKANPKYWGTEHKARTENVIVRFLPDENASINALKSGDVQVLSPI
CCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEECCH
SATLADPFRKDPNYVVKSGDGSDKFVLAFNMANDKLSDKRVRQAIRYAIDHKQIIASRGG
HHHHHHHHHCCCCEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VDAPLGGPIPKVDPGYEDLSGLYPHDVAKAKKLMEQAGYSESKPLDLTLTYANVYGTELG
CCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHH
DQLKSQLKPIGIDLKINYVEFSTWLQDVHANGNYDLSLVDHAESHDFYGWTTPSYYFHYD
HHHHHHCCCEEEEEEEEEEEHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEEC
NKDVQALYAKALEATSDDEASKYLAKAAKIVSEDAPADWLFSWKVTIAYAKGVDGFPQNL
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCHHH
SQTVLPLWRITYTK
HHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA