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The map label for this gene is metB [H]

Identifier: 213692862

GI number: 213692862

Start: 2187465

End: 2188196

Strand: Reverse

Name: metB [H]

Synonym: Blon_2003

Alternate gene names: 213692862

Gene position: 2188196-2187465 (Counterclockwise)

Preceding gene: 213692863

Following gene: 213692860

Centisome position: 77.25

GC content: 66.39

Gene sequence:

>732_bases
GTGTGGGTCGAGACCCCGTCCAACCCGCTGCTCAACATCACCGACATCGCGGCCACCGCCGAAGTTGCCCACAAGTACGG
CACCAAGGTCGCGGTCGACAACACCTTCGCCTCGCCCGCCCTTCAGCACCCGCTGGCCGATGGCGCCGATGTGGTGGTCT
ACTCCACCACCAAGTACATCGGCGGCCACTCCGACGTGGTCGGCGGTGCCGTGGTGGTCAACGACGAGGAGACCCGCGAG
AAGGTCGCCTTCCTGCAGAACGCCGCCGGCGCAGTGCCCTCCCCGTTCGACTCCTGGCTGGACATCCGCGGCCTGAAGAC
CCTGGATCTGCGCGTCAAGCGCCACAGTGCCAACGCCCTGAAGGTGGCCGAATGGCTCGAATCCCAGCCCTCTGATGTGA
TTGAGCGCGTCTGGTACCCGGGCCTCGAGTCCCACCCTGCTCATGAAATCGCCGCCCGCCAGATGCACGGCGGCTTCGGC
GGCATCGTCTCCGTGCAGCTCGCGGGCGGTGCCGAGGCGGCCAAGCACTTCGTGGACCACACGCAGATCTTCACACTGGC
CGAATCGCTCGGCGGTGTGGAAAGCCTCATCGAGGTGCCCGCCGCCATGACCCACGCCTCCGTGGCCGGCACCACCCTGC
AGGTGCCCGCCAACCTGGTGCGTATCTCGGTCGGTATCGAGAACGCCGACGACCTTATCGCCGATCTCAAGCAGGCGTTG
GATCGTATCTGA

Upstream 100 bases:

>100_bases
CGGAGCTCCCTCTGATGAGGGAGCTGGGGACGGACTCCATAACTAGCTATCAAAAGAATCAATACCAATATCCGCACTGG
CCTCCAAGCACTACCAGTAT

Downstream 100 bases:

>100_bases
CTGTACTGACGGTACTGGTGGCACCGTATTGACGGTGCCACCACGCTTATCGGCGGACTCGCTATCGATTGGTACCTACA
AAAAACCCCGGCCCACAAGG

Product: Cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MWVETPSNPLLNITDIAATAEVAHKYGTKVAVDNTFASPALQHPLADGADVVVYSTTKYIGGHSDVVGGAVVVNDEETRE
KVAFLQNAAGAVPSPFDSWLDIRGLKTLDLRVKRHSANALKVAEWLESQPSDVIERVWYPGLESHPAHEIAARQMHGGFG
GIVSVQLAGGAEAAKHFVDHTQIFTLAESLGGVESLIEVPAAMTHASVAGTTLQVPANLVRISVGIENADDLIADLKQAL
DRI

Sequences:

>Translated_243_residues
MWVETPSNPLLNITDIAATAEVAHKYGTKVAVDNTFASPALQHPLADGADVVVYSTTKYIGGHSDVVGGAVVVNDEETRE
KVAFLQNAAGAVPSPFDSWLDIRGLKTLDLRVKRHSANALKVAEWLESQPSDVIERVWYPGLESHPAHEIAARQMHGGFG
GIVSVQLAGGAEAAKHFVDHTQIFTLAESLGGVESLIEVPAAMTHASVAGTTLQVPANLVRISVGIENADDLIADLKQAL
DRI
>Mature_243_residues
MWVETPSNPLLNITDIAATAEVAHKYGTKVAVDNTFASPALQHPLADGADVVVYSTTKYIGGHSDVVGGAVVVNDEETRE
KVAFLQNAAGAVPSPFDSWLDIRGLKTLDLRVKRHSANALKVAEWLESQPSDVIERVWYPGLESHPAHEIAARQMHGGFG
GIVSVQLAGGAEAAKHFVDHTQIFTLAESLGGVESLIEVPAAMTHASVAGTTLQVPANLVRISVGIENADDLIADLKQAL
DRI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=244, Percent_Identity=50.4098360655738, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI299473759, Length=244, Percent_Identity=50.4098360655738, Blast_Score=224, Evalue=4e-59,
Organism=Homo sapiens, GI25453487, Length=231, Percent_Identity=46.7532467532467, Blast_Score=185, Evalue=2e-47,
Organism=Escherichia coli, GI1790375, Length=243, Percent_Identity=45.2674897119342, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1789383, Length=252, Percent_Identity=31.3492063492063, Blast_Score=111, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17538055, Length=245, Percent_Identity=53.469387755102, Blast_Score=233, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17560056, Length=245, Percent_Identity=51.0204081632653, Blast_Score=226, Evalue=8e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=246, Percent_Identity=44.3089430894309, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=250, Percent_Identity=50, Blast_Score=245, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6321904, Length=244, Percent_Identity=36.0655737704918, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321254, Length=253, Percent_Identity=35.5731225296443, Blast_Score=141, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6323333, Length=290, Percent_Identity=32.4137931034483, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=194, Percent_Identity=34.5360824742268, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322970, Length=256, Percent_Identity=27.734375, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6322590, Length=252, Percent_Identity=25.3968253968254, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323557, Length=262, Percent_Identity=25.1908396946565, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19922554, Length=246, Percent_Identity=49.1869918699187, Blast_Score=241, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 25811; Mature: 25811

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWVETPSNPLLNITDIAATAEVAHKYGTKVAVDNTFASPALQHPLADGADVVVYSTTKYI
CCCCCCCCCCEEHHHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCCCCCEEEEECCHHC
GGHSDVVGGAVVVNDEETREKVAFLQNAAGAVPSPFDSWLDIRGLKTLDLRVKRHSANAL
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEEECCCCCHH
KVAEWLESQPSDVIERVWYPGLESHPAHEIAARQMHGGFGGIVSVQLAGGAEAAKHFVDH
HHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
TQIFTLAESLGGVESLIEVPAAMTHASVAGTTLQVPANLVRISVGIENADDLIADLKQAL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHH
DRI
HCC
>Mature Secondary Structure
MWVETPSNPLLNITDIAATAEVAHKYGTKVAVDNTFASPALQHPLADGADVVVYSTTKYI
CCCCCCCCCCEEHHHHHHHHHHHHHHCCEEEECCCCCCHHHHCCCCCCCCEEEEECCHHC
GGHSDVVGGAVVVNDEETREKVAFLQNAAGAVPSPFDSWLDIRGLKTLDLRVKRHSANAL
CCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEEEECCCCCHH
KVAEWLESQPSDVIERVWYPGLESHPAHEIAARQMHGGFGGIVSVQLAGGAEAAKHFVDH
HHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHH
TQIFTLAESLGGVESLIEVPAAMTHASVAGTTLQVPANLVRISVGIENADDLIADLKQAL
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHH
DRI
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]