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The map label for this gene is dnaG [H]

Identifier: 213692854

GI number: 213692854

Start: 2175134

End: 2177233

Strand: Reverse

Name: dnaG [H]

Synonym: Blon_1995

Alternate gene names: 213692854

Gene position: 2177233-2175134 (Counterclockwise)

Preceding gene: 213692855

Following gene: 213692853

Centisome position: 76.86

GC content: 63.05

Gene sequence:

>2100_bases
ATGGTCGGAATGATTAAGAAAGAAGATGTCGAGAAGGTGCGCGCCGCCGCGGACCTGTATGACATTGTATCTGCGACCGT
AACGCTTAAGCCCTCCGGCACCGGCACCTTCGTGGGCCTGTGCCCGTTCCACGACGAGAAAACCGGCAGCTTCAACGTGC
GTCCCTCGCTCGGCGTGTGGCACTGCTTCGGCTGTGGCTTGGGTGGCGACGTATTCAAATACGTCGAACAATCCGAAAAC
ATCGACTTCCGCGAAGCCGTGGAACTGCTCGCCGACAAGTACCATATCGAGCTGCACTACGAGAACAGCGGCAACGGCCC
GAGCCGCGAGAACACCGGCTCCAAGCGCACGCGACTGCTGGAAGCCTGCGAGGAAGCCCAGCGCTTCTTCGTCTCCCAGA
TCCTGACCAAGGAAGCGCTGCCCGCCCGCAAGCTGCTTGGCGGCCGCAACTTCTCCCAAGCTGATTGCGAACGTTTCGGT
TGCGGCTACGCGCCCCAAGGTTGGGATAATCTTGTGCGCCACTTGGCCTCCAAGGGCTTCACGCAGAAGGAAATCCTCGA
CGCGGGCTTGGCCCGTCAAGGCCAGCGCGGCATCTATGACTATTTCCGTGGCCGCGTGACGTGGCCTATCCGCGATTCGA
CCGGCCGCACGCTCGGCTTCGGTGCGCGCAAATTATACGAAGACGACCAGATCGCCGCCAAATACATCAACACGCCGGAC
ACGCAGCTCTACCGCAAAACCCAGGTGCTTTACGGCATCGATTTGGCGAAATCCGCCATCGTCAAGAAGCGGCAGGTCGT
GATCGTCGAAGGCTATACGGACGTGATGGCCATGCACTTGGCCGGCATCGACACCGCCATCGCCACGTGCGGCACGGCGT
TCGGCGCCGAGCACGCCAAGATTGTGCGCCGCCTGATCGCCGACGATTCGCTGGGCGCGGTCCAGCTGGTCGGGCCGCTG
AAGGTCAAGGACCAGCCATTGAGCTCGCGTATTGTGTTCACGTTCGACGGTGACGCTGCCGGTCATAAGGCCGCGCTGCA
CGCCTTCGGCCTTGATTCGGCCTTCCTCTCCCAAACCTTCGTGGCCGTGGCCGACGACAATCTTGACCCGTGCGACCTGC
GCATCGAGCGAGGCAACGAGGCGGTGCGCTCGCTGATCGCCCGCGCCAAACCGTTGTATGATTTCGTGATCGACGCCGCC
ATCAGCCGGTTCGACCTGACCTACACGCCCGGCCAGGTGGGTGCGATGAAAGCCGTGGCCCCGCTGGTGGCGCAGATTCG
CGACCGCTCCCTGTGGGACGCTTACGCGCGCAAGTCCGCGGGCCGTATCGGCGTGGACCTTGAGGTGATGCGCCGCGAGG
TGATGAACGCGCGCCGGCAGATGCACGTGCGCGATGAGGACGCCTATGCGCCCAAACGTCGATTCGAGCGTGACGAGCCG
CGTGTGGAACCCGGCACCAACCCGTACACCAATCCGGCCACGCGCAAGGCTTTGGAGCGCCGTGACGCCGCCGAACAGGC
ATATTTCAAGATCGACGACGCCGTATTCATCGCCGAACAGCAGTTCATGGCCGTGTTGATTCAGGTGCCGCGCGCCATCG
ACCGCATCATGTTCGGGCAGCTGACCATCGAGCATTTCATGACTTCGGTGTTCCGCACCCTGTTCCAGGCGATCGCGGCC
GCTGGCGGACTGCCATCCGACGATACCCCGCAGGGGCTGTGGATGCACAACCTCACCAAGGCAGGCGGGCCGATGCTCAA
TCAGGTAATCAACGAGCTGGCCGTCATGCCGTTGCCACTGCCTGGCGACGACGGTGGTAATGGCGCACAACCTCCGCAGT
CGAACGCGCCCGGGGGAGCCGGCGGTGGTGCGGCCGCCGTGCAGCTACGCCCTGCCACGCCGGAAGAGCAGCGCTATGCC
ACCGAACTGCTCACCCGACTACTGGACATGGGCTTCATGCGCCAGATCGGCTTGGCCAAACGCCGCATGGCCCAGCTGCC
CGACGGCGAAGAAAAAATAACCCTCCTCGGCCAGATCACCCGCATGGAAACCGCCCGTAAAGACCTCCAAGCCCAAATCT
ACGGCAACACCGTCGGCTAA

Upstream 100 bases:

>100_bases
AAGGGGCCTCGCTGAGTCTGAGGGGAGTCGAACCCAGAAGAACTGGTGTCCAACGGTAAAAATTTACTGTGTCTCAGGTG
AGGCGGTACTCTAGAGACTT

Downstream 100 bases:

>100_bases
TACCTCCCACTGATTGCGCGCATCTTCGAATACGTGCCGGATGCATAAGGCCATACAACCGGTTGCCGGATTGGATGGGT
GCAGAAGTTGCGGATTGGCC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MVGMIKKEDVEKVRAAADLYDIVSATVTLKPSGTGTFVGLCPFHDEKTGSFNVRPSLGVWHCFGCGLGGDVFKYVEQSEN
IDFREAVELLADKYHIELHYENSGNGPSRENTGSKRTRLLEACEEAQRFFVSQILTKEALPARKLLGGRNFSQADCERFG
CGYAPQGWDNLVRHLASKGFTQKEILDAGLARQGQRGIYDYFRGRVTWPIRDSTGRTLGFGARKLYEDDQIAAKYINTPD
TQLYRKTQVLYGIDLAKSAIVKKRQVVIVEGYTDVMAMHLAGIDTAIATCGTAFGAEHAKIVRRLIADDSLGAVQLVGPL
KVKDQPLSSRIVFTFDGDAAGHKAALHAFGLDSAFLSQTFVAVADDNLDPCDLRIERGNEAVRSLIARAKPLYDFVIDAA
ISRFDLTYTPGQVGAMKAVAPLVAQIRDRSLWDAYARKSAGRIGVDLEVMRREVMNARRQMHVRDEDAYAPKRRFERDEP
RVEPGTNPYTNPATRKALERRDAAEQAYFKIDDAVFIAEQQFMAVLIQVPRAIDRIMFGQLTIEHFMTSVFRTLFQAIAA
AGGLPSDDTPQGLWMHNLTKAGGPMLNQVINELAVMPLPLPGDDGGNGAQPPQSNAPGGAGGGAAAVQLRPATPEEQRYA
TELLTRLLDMGFMRQIGLAKRRMAQLPDGEEKITLLGQITRMETARKDLQAQIYGNTVG

Sequences:

>Translated_699_residues
MVGMIKKEDVEKVRAAADLYDIVSATVTLKPSGTGTFVGLCPFHDEKTGSFNVRPSLGVWHCFGCGLGGDVFKYVEQSEN
IDFREAVELLADKYHIELHYENSGNGPSRENTGSKRTRLLEACEEAQRFFVSQILTKEALPARKLLGGRNFSQADCERFG
CGYAPQGWDNLVRHLASKGFTQKEILDAGLARQGQRGIYDYFRGRVTWPIRDSTGRTLGFGARKLYEDDQIAAKYINTPD
TQLYRKTQVLYGIDLAKSAIVKKRQVVIVEGYTDVMAMHLAGIDTAIATCGTAFGAEHAKIVRRLIADDSLGAVQLVGPL
KVKDQPLSSRIVFTFDGDAAGHKAALHAFGLDSAFLSQTFVAVADDNLDPCDLRIERGNEAVRSLIARAKPLYDFVIDAA
ISRFDLTYTPGQVGAMKAVAPLVAQIRDRSLWDAYARKSAGRIGVDLEVMRREVMNARRQMHVRDEDAYAPKRRFERDEP
RVEPGTNPYTNPATRKALERRDAAEQAYFKIDDAVFIAEQQFMAVLIQVPRAIDRIMFGQLTIEHFMTSVFRTLFQAIAA
AGGLPSDDTPQGLWMHNLTKAGGPMLNQVINELAVMPLPLPGDDGGNGAQPPQSNAPGGAGGGAAAVQLRPATPEEQRYA
TELLTRLLDMGFMRQIGLAKRRMAQLPDGEEKITLLGQITRMETARKDLQAQIYGNTVG
>Mature_699_residues
MVGMIKKEDVEKVRAAADLYDIVSATVTLKPSGTGTFVGLCPFHDEKTGSFNVRPSLGVWHCFGCGLGGDVFKYVEQSEN
IDFREAVELLADKYHIELHYENSGNGPSRENTGSKRTRLLEACEEAQRFFVSQILTKEALPARKLLGGRNFSQADCERFG
CGYAPQGWDNLVRHLASKGFTQKEILDAGLARQGQRGIYDYFRGRVTWPIRDSTGRTLGFGARKLYEDDQIAAKYINTPD
TQLYRKTQVLYGIDLAKSAIVKKRQVVIVEGYTDVMAMHLAGIDTAIATCGTAFGAEHAKIVRRLIADDSLGAVQLVGPL
KVKDQPLSSRIVFTFDGDAAGHKAALHAFGLDSAFLSQTFVAVADDNLDPCDLRIERGNEAVRSLIARAKPLYDFVIDAA
ISRFDLTYTPGQVGAMKAVAPLVAQIRDRSLWDAYARKSAGRIGVDLEVMRREVMNARRQMHVRDEDAYAPKRRFERDEP
RVEPGTNPYTNPATRKALERRDAAEQAYFKIDDAVFIAEQQFMAVLIQVPRAIDRIMFGQLTIEHFMTSVFRTLFQAIAA
AGGLPSDDTPQGLWMHNLTKAGGPMLNQVINELAVMPLPLPGDDGGNGAQPPQSNAPGGAGGGAAAVQLRPATPEEQRYA
TELLTRLLDMGFMRQIGLAKRRMAQLPDGEEKITLLGQITRMETARKDLQAQIYGNTVG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=450, Percent_Identity=30.6666666666667, Blast_Score=223, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 77010; Mature: 77010

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGMIKKEDVEKVRAAADLYDIVSATVTLKPSGTGTFVGLCPFHDEKTGSFNVRPSLGVW
CCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCCCCCEECCCCCCCH
HCFGCGLGGDVFKYVEQSENIDFREAVELLADKYHIELHYENSGNGPSRENTGSKRTRLL
HHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHH
EACEEAQRFFVSQILTKEALPARKLLGGRNFSQADCERFGCGYAPQGWDNLVRHLASKGF
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCC
TQKEILDAGLARQGQRGIYDYFRGRVTWPIRDSTGRTLGFGARKLYEDDQIAAKYINTPD
CHHHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCEECCCHHHHCCCHHHHHHHCCCCC
TQLYRKTQVLYGIDLAKSAIVKKRQVVIVEGYTDVMAMHLAGIDTAIATCGTAFGAEHAK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IVRRLIADDSLGAVQLVGPLKVKDQPLSSRIVFTFDGDAAGHKAALHAFGLDSAFLSQTF
HHHHHHCCCCCCCEEEECCCEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHE
VAVADDNLDPCDLRIERGNEAVRSLIARAKPLYDFVIDAAISRFDLTYTPGQVGAMKAVA
EEEECCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHH
PLVAQIRDRSLWDAYARKSAGRIGVDLEVMRREVMNARRQMHVRDEDAYAPKRRFERDEP
HHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
RVEPGTNPYTNPATRKALERRDAAEQAYFKIDDAVFIAEQQFMAVLIQVPRAIDRIMFGQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LTIEHFMTSVFRTLFQAIAAAGGLPSDDTPQGLWMHNLTKAGGPMLNQVINELAVMPLPL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PGDDGGNGAQPPQSNAPGGAGGGAAAVQLRPATPEEQRYATELLTRLLDMGFMRQIGLAK
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRMAQLPDGEEKITLLGQITRMETARKDLQAQIYGNTVG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure
MVGMIKKEDVEKVRAAADLYDIVSATVTLKPSGTGTFVGLCPFHDEKTGSFNVRPSLGVW
CCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCCCCCCCCCEECCCCCCCH
HCFGCGLGGDVFKYVEQSENIDFREAVELLADKYHIELHYENSGNGPSRENTGSKRTRLL
HHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCHHHHHHH
EACEEAQRFFVSQILTKEALPARKLLGGRNFSQADCERFGCGYAPQGWDNLVRHLASKGF
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCC
TQKEILDAGLARQGQRGIYDYFRGRVTWPIRDSTGRTLGFGARKLYEDDQIAAKYINTPD
CHHHHHHHHHHHCCCCCHHHHHCCEEEEEEECCCCCEECCCHHHHCCCHHHHHHHCCCCC
TQLYRKTQVLYGIDLAKSAIVKKRQVVIVEGYTDVMAMHLAGIDTAIATCGTAFGAEHAK
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
IVRRLIADDSLGAVQLVGPLKVKDQPLSSRIVFTFDGDAAGHKAALHAFGLDSAFLSQTF
HHHHHHCCCCCCCEEEECCCEECCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHE
VAVADDNLDPCDLRIERGNEAVRSLIARAKPLYDFVIDAAISRFDLTYTPGQVGAMKAVA
EEEECCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCEEECCCCHHHHHHHH
PLVAQIRDRSLWDAYARKSAGRIGVDLEVMRREVMNARRQMHVRDEDAYAPKRRFERDEP
HHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCC
RVEPGTNPYTNPATRKALERRDAAEQAYFKIDDAVFIAEQQFMAVLIQVPRAIDRIMFGQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHH
LTIEHFMTSVFRTLFQAIAAAGGLPSDDTPQGLWMHNLTKAGGPMLNQVINELAVMPLPL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PGDDGGNGAQPPQSNAPGGAGGGAAAVQLRPATPEEQRYATELLTRLLDMGFMRQIGLAK
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRMAQLPDGEEKITLLGQITRMETARKDLQAQIYGNTVG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]