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The map label for this gene is purK [H]

Identifier: 213692839

GI number: 213692839

Start: 2156934

End: 2158112

Strand: Reverse

Name: purK [H]

Synonym: Blon_1980

Alternate gene names: 213692839

Gene position: 2158112-2156934 (Counterclockwise)

Preceding gene: 213692840

Following gene: 213692838

Centisome position: 76.18

GC content: 63.61

Gene sequence:

>1179_bases
ATGCCCAGCTTGTCTGAGGAAACAGGCGGTCGTGTTTCGATGCTGATGCCGGGATCGACCATCGGCATCATCGGCGGCGG
CCAGCTCGGCCGCATGATGGCTTTGTCCGCCCGCTACATGGGCTTCCGCGTCGGCGTGCTTGACCCGACGCCGAACTGCC
CGACCGGTCAGGTGGCCGACTTCCAGATCACCGCCGAATACGATGACAAAACCGCCATCCATGATCTGGCCGTGCAGTCC
GACGTGCTCACCTACGAGTTCGAGAACGTGGACGCCGATGCCATCGACGAGGTGCGCGGCATCACCGCCACCCCGCAGGG
CACTGACCTGCTGCGCGTGACCCAGCACCGCGTGCACGAGAAGCAGTTCATCAACGACCACGGCACCGCCACCGCACCGT
GGCACGCTGTCAACAACTTCGATGAGCTTGACGCCGCATTGGATGATATTCATTATCCGGCCGTGCTGAAGACCGTGTCC
GGCGGCTACGACGGCCACGGTCAGCTCGTGCTGCGTTCCGACGCCGACTTGGAGAAGATCCGCTCCCGTTCCGACCGTGG
TGGCGGCTTCCCGCCGAGCATCCTGGAAGGCTTCGTCGACTTCGCATTCGAGGCTTCGATCCTGGTCTCCGGCAACGGCA
AGGACTTCGTCACCTTCCCCATCGTGCGTAACGAGCACCGCAACAACATCCTGCACATGACCATCGCCCCCGCCGAAGTG
TCCGAACATGTGGCTCGCGAAGCGCATGAACTGGCGATTCGCTTGGCCAAGGGCTTCGAACTGGCTGGCACGCTGGCCAT
CGAACTGTTCATCACCAAGGACGATCAGGTCATCGTCAACGAGCTCGCCCCGCGCCCGCACAACTCCGGCCACTACACCA
TCGAGGCCTGCTCGATGGACCAGTTCGACGCCCACATCCGCGGCATCGCCGGCTGGCCCATGCCCAAGCCCAAGCTGCTC
TCGCCGGCCGTGATGGTCAACGTACTCGGCCAGCATGTGGAGCCGACGCGCGCGCTGATCGCCACCCACCCGGAGTGGAA
TGTGCACGACTACGGCAAGGCCGAAGTGCGCCACGACCGCAAGATGGGCCACATCACCGTGCTCACCGACAACCCTGATG
CCACGGTTGCTGACCTTGAGGCCACCGGCTGCTGGGACGACCTGAAAAAGAAGGCGTGA

Upstream 100 bases:

>100_bases
GGCCTGCTCGCCGTGCAGATGCTCTCCGCCTTCGAGCCCGAACTGGCCGACAAGCTTGAGGATTACCGTAACGAACTGAA
AGAAAAGGTGGCTGAATCCA

Downstream 100 bases:

>100_bases
CCTTATCGATGGTTGAACATATCGAACGCCAGACCAAGCAGAAGGACGCGATTCGCGCCGCCTTGGCCGACTGTGAGGAG
TTCATCTCCGCTCAGGACCT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPSLSEETGGRVSMLMPGSTIGIIGGGQLGRMMALSARYMGFRVGVLDPTPNCPTGQVADFQITAEYDDKTAIHDLAVQS
DVLTYEFENVDADAIDEVRGITATPQGTDLLRVTQHRVHEKQFINDHGTATAPWHAVNNFDELDAALDDIHYPAVLKTVS
GGYDGHGQLVLRSDADLEKIRSRSDRGGGFPPSILEGFVDFAFEASILVSGNGKDFVTFPIVRNEHRNNILHMTIAPAEV
SEHVAREAHELAIRLAKGFELAGTLAIELFITKDDQVIVNELAPRPHNSGHYTIEACSMDQFDAHIRGIAGWPMPKPKLL
SPAVMVNVLGQHVEPTRALIATHPEWNVHDYGKAEVRHDRKMGHITVLTDNPDATVADLEATGCWDDLKKKA

Sequences:

>Translated_392_residues
MPSLSEETGGRVSMLMPGSTIGIIGGGQLGRMMALSARYMGFRVGVLDPTPNCPTGQVADFQITAEYDDKTAIHDLAVQS
DVLTYEFENVDADAIDEVRGITATPQGTDLLRVTQHRVHEKQFINDHGTATAPWHAVNNFDELDAALDDIHYPAVLKTVS
GGYDGHGQLVLRSDADLEKIRSRSDRGGGFPPSILEGFVDFAFEASILVSGNGKDFVTFPIVRNEHRNNILHMTIAPAEV
SEHVAREAHELAIRLAKGFELAGTLAIELFITKDDQVIVNELAPRPHNSGHYTIEACSMDQFDAHIRGIAGWPMPKPKLL
SPAVMVNVLGQHVEPTRALIATHPEWNVHDYGKAEVRHDRKMGHITVLTDNPDATVADLEATGCWDDLKKKA
>Mature_391_residues
PSLSEETGGRVSMLMPGSTIGIIGGGQLGRMMALSARYMGFRVGVLDPTPNCPTGQVADFQITAEYDDKTAIHDLAVQSD
VLTYEFENVDADAIDEVRGITATPQGTDLLRVTQHRVHEKQFINDHGTATAPWHAVNNFDELDAALDDIHYPAVLKTVSG
GYDGHGQLVLRSDADLEKIRSRSDRGGGFPPSILEGFVDFAFEASILVSGNGKDFVTFPIVRNEHRNNILHMTIAPAEVS
EHVAREAHELAIRLAKGFELAGTLAIELFITKDDQVIVNELAPRPHNSGHYTIEACSMDQFDAHIRGIAGWPMPKPKLLS
PAVMVNVLGQHVEPTRALIATHPEWNVHDYGKAEVRHDRKMGHITVLTDNPDATVADLEATGCWDDLKKKA

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=363, Percent_Identity=29.4765840220386, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788155, Length=365, Percent_Identity=24.6575342465753, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324702, Length=381, Percent_Identity=32.8083989501312, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42860; Mature: 42729

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSLSEETGGRVSMLMPGSTIGIIGGGQLGRMMALSARYMGFRVGVLDPTPNCPTGQVAD
CCCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEE
FQITAEYDDKTAIHDLAVQSDVLTYEFENVDADAIDEVRGITATPQGTDLLRVTQHRVHE
EEEEECCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KQFINDHGTATAPWHAVNNFDELDAALDDIHYPAVLKTVSGGYDGHGQLVLRSDADLEKI
HHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCHHHH
RSRSDRGGGFPPSILEGFVDFAFEASILVSGNGKDFVTFPIVRNEHRNNILHMTIAPAEV
HCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCEEEEEEEECCCCCCEEEEEECHHHH
SEHVAREAHELAIRLAKGFELAGTLAIELFITKDDQVIVNELAPRPHNSGHYTIEACSMD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCEEHHHHCCCCCCCCCCEEEEECCCC
QFDAHIRGIAGWPMPKPKLLSPAVMVNVLGQHVEPTRALIATHPEWNVHDYGKAEVRHDR
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHCC
KMGHITVLTDNPDATVADLEATGCWDDLKKKA
CCCEEEEEECCCCCEEEECCCCCCHHHHHHCC
>Mature Secondary Structure 
PSLSEETGGRVSMLMPGSTIGIIGGGQLGRMMALSARYMGFRVGVLDPTPNCPTGQVAD
CCCCCCCCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEE
FQITAEYDDKTAIHDLAVQSDVLTYEFENVDADAIDEVRGITATPQGTDLLRVTQHRVHE
EEEEECCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
KQFINDHGTATAPWHAVNNFDELDAALDDIHYPAVLKTVSGGYDGHGQLVLRSDADLEKI
HHHHCCCCCCCCCCHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCHHHH
RSRSDRGGGFPPSILEGFVDFAFEASILVSGNGKDFVTFPIVRNEHRNNILHMTIAPAEV
HCCCCCCCCCCHHHHHHHHHHHEEEEEEEECCCCCEEEEEEEECCCCCCEEEEEECHHHH
SEHVAREAHELAIRLAKGFELAGTLAIELFITKDDQVIVNELAPRPHNSGHYTIEACSMD
HHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCEEHHHHCCCCCCCCCCEEEEECCCC
QFDAHIRGIAGWPMPKPKLLSPAVMVNVLGQHVEPTRALIATHPEWNVHDYGKAEVRHDR
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCHHHHHCC
KMGHITVLTDNPDATVADLEATGCWDDLKKKA
CCCEEEEEECCCCCEEEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]