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The map label for this gene is glnH [H]

Identifier: 213692815

GI number: 213692815

Start: 2135841

End: 2136704

Strand: Direct

Name: glnH [H]

Synonym: Blon_1955

Alternate gene names: 213692815

Gene position: 2135841-2136704 (Clockwise)

Preceding gene: 213692814

Following gene: 213692816

Centisome position: 75.4

GC content: 59.72

Gene sequence:

>864_bases
ATGTTCAATAAGCAACACATCGTCCGTACCATTGCGTCCACCGCCAGCATCCTGGCTCTGTCGTTCACCGCAGCCTGCGG
TTCCGGCCAGTCCACCGCATCCAATTCCGCCGATTCGAACGACATCACCCAGCAGACGTACAAGCCGGGCAAGCTGACCA
TCGCCACCGGACAGCCGGCCTACGAGCCGTGGGTTATGAACGACAAGCCGGAAAGCGGTGAGGGCTACGAGGCGGCCGTC
GCCTACGCAGTGGCCGACAAGCTCGGTTTCAAGAAGAGCGACGTGGTGTGGACCCGCACCGCATTCGACACCGCCATCGC
TCCCGGCGCCAAGGACTGGGATCTCAACATCCAGCAGTTCGGCATCACCGACGAGCGCAAGAAGGCCGTTGACTTCTCGT
CCAGCTACTACAACGACAGCCAGTCCGTCGTGGTCAAGAAAGACAGCAAGTTCGCCAACGCCACCAAGGTATCCGATCTG
AAGAATGCGACCGTGGGCGTCATGGTCGGCACCCTTGCCTACGACTACGCCAAGGCGAACATCAAGGACGACATCCAGAC
GTTCAATGATGATGCCGCACTGGCTCAGGCACTTGACGCCGGTCAAATCGACGCACTGGTCACCAGCACCGTGGAATGCG
TGTACATGGTGCAGTCCGAGCAGGTCAAGGACGCCAAGGTGCTCGGCCGTCTGCCTGACTCCGAAGACAAGAGCGGCATG
GGCATCGTGCTGCCGAAGGATTCCAAGCTAACTGCAGCCGTGACCAAGGCAGTGGATGACCTGAACGCCGACGGCACCAT
CAAGAAGCTTCAGGACAAGTGGCTGGCCGAGTACACCACCGACCTGACCGAACTGAAGAAGTAA

Upstream 100 bases:

>100_bases
TCACTGTTTTGTAGTAACCCAATAGCCATACAATTGGCGCGCATGCATCAAGCACGACTTTGAACCGTGTGCATGACGAA
CACTTGAGGAGAGGAAGCCC

Downstream 100 bases:

>100_bases
GTGGAGTAGGCTCACATCATAAGGTCACAACACCTTGGCCGGACGCTGCGGAAATTTTCCTGTGACACCCGGCCATTCCG
GCATTCAGGAAGCGAGCGAC

Product: extracellular solute-binding protein, family 3

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: GlnBP [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MFNKQHIVRTIASTASILALSFTAACGSGQSTASNSADSNDITQQTYKPGKLTIATGQPAYEPWVMNDKPESGEGYEAAV
AYAVADKLGFKKSDVVWTRTAFDTAIAPGAKDWDLNIQQFGITDERKKAVDFSSSYYNDSQSVVVKKDSKFANATKVSDL
KNATVGVMVGTLAYDYAKANIKDDIQTFNDDAALAQALDAGQIDALVTSTVECVYMVQSEQVKDAKVLGRLPDSEDKSGM
GIVLPKDSKLTAAVTKAVDDLNADGTIKKLQDKWLAEYTTDLTELKK

Sequences:

>Translated_287_residues
MFNKQHIVRTIASTASILALSFTAACGSGQSTASNSADSNDITQQTYKPGKLTIATGQPAYEPWVMNDKPESGEGYEAAV
AYAVADKLGFKKSDVVWTRTAFDTAIAPGAKDWDLNIQQFGITDERKKAVDFSSSYYNDSQSVVVKKDSKFANATKVSDL
KNATVGVMVGTLAYDYAKANIKDDIQTFNDDAALAQALDAGQIDALVTSTVECVYMVQSEQVKDAKVLGRLPDSEDKSGM
GIVLPKDSKLTAAVTKAVDDLNADGTIKKLQDKWLAEYTTDLTELKK
>Mature_287_residues
MFNKQHIVRTIASTASILALSFTAACGSGQSTASNSADSNDITQQTYKPGKLTIATGQPAYEPWVMNDKPESGEGYEAAV
AYAVADKLGFKKSDVVWTRTAFDTAIAPGAKDWDLNIQQFGITDERKKAVDFSSSYYNDSQSVVVKKDSKFANATKVSDL
KNATVGVMVGTLAYDYAKANIKDDIQTFNDDAALAQALDAGQIDALVTSTVECVYMVQSEQVKDAKVLGRLPDSEDKSGM
GIVLPKDSKLTAAVTKAVDDLNADGTIKKLQDKWLAEYTTDLTELKK

Specific function: Involved in a glutamine-transport system glnHPQ [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=169, Percent_Identity=36.094674556213, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=29.2792792792793, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787085, Length=216, Percent_Identity=27.3148148148148, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30929; Mature: 30929

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKQHIVRTIASTASILALSFTAACGSGQSTASNSADSNDITQQTYKPGKLTIATGQPA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCC
YEPWVMNDKPESGEGYEAAVAYAVADKLGFKKSDVVWTRTAFDTAIAPGAKDWDLNIQQF
CCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCHHEE
GITDERKKAVDFSSSYYNDSQSVVVKKDSKFANATKVSDLKNATVGVMVGTLAYDYAKAN
CCCHHHHHHHCCCCHHCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
IKDDIQTFNDDAALAQALDAGQIDALVTSTVECVYMVQSEQVKDAKVLGRLPDSEDKSGM
CHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCHHHHHHCCCCCCCCCCCC
GIVLPKDSKLTAAVTKAVDDLNADGTIKKLQDKWLAEYTTDLTELKK
EEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFNKQHIVRTIASTASILALSFTAACGSGQSTASNSADSNDITQQTYKPGKLTIATGQPA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCEEEEECCCCC
YEPWVMNDKPESGEGYEAAVAYAVADKLGFKKSDVVWTRTAFDTAIAPGAKDWDLNIQQF
CCCEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHCCCCCCCCCCHHEE
GITDERKKAVDFSSSYYNDSQSVVVKKDSKFANATKVSDLKNATVGVMVGTLAYDYAKAN
CCCHHHHHHHCCCCHHCCCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCC
IKDDIQTFNDDAALAQALDAGQIDALVTSTVECVYMVQSEQVKDAKVLGRLPDSEDKSGM
CHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHEEEEECCCCCHHHHHHCCCCCCCCCCCC
GIVLPKDSKLTAAVTKAVDDLNADGTIKKLQDKWLAEYTTDLTELKK
EEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]