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The map label for this gene is phoR [H]

Identifier: 213692772

GI number: 213692772

Start: 2083202

End: 2083921

Strand: Reverse

Name: phoR [H]

Synonym: Blon_1908

Alternate gene names: 213692772

Gene position: 2083921-2083202 (Counterclockwise)

Preceding gene: 213692774

Following gene: 213692769

Centisome position: 73.57

GC content: 58.06

Gene sequence:

>720_bases
ATGTTCGCCGATTTCAATACCATGGTCGAGGAACTGGGCGGCATCGAAACCATGAAAAACGACTTCGTCTCCAATGTGAG
CCACGAAATCCGCAATCCTCTGGCCGCCATCAAGAATTACGCGACCATGATGCGTGACATGGATATGGACAAGGCCCAGC
GCGAGGAATGCGTACGGACCATCATCTCCGCGTCCGAACGACTCAACGCGCTCGTGACCAACATCCTCAAACTCAACAAA
CTCGAAAGCCAGACCATCGTCTCCCAGGCCGTCGACTACGACCTGACCCGTCAGCTGACCGACGAGATCCTCACGCTCGA
CGACCTGTTCACCGAAAAGAACATCGATTTGGGCTGCGACCTGGAAGACCGTGCGATGGTGCACGCCGACCTTGGCATCA
CCACGCTCATCTGGAGCAATATCCTCGGCAACGCGCTCAAATACACCGAATCAGGCGGCCATGTGAGCTTCACACAGCGC
TCGCACGGCAATATGGTGTCGGTGGAGATCAGCGATGACGGCTGCGACATGACCCCGGACGAAGCCGCCCACATGTTCGA
CAAGTTCTATCAGGGCGATACCTCGCACGCCGCACAAGGCAACGGCCTCGGCATGGCGATGGTCAAGCGCGCCGTGGACC
TGAGCCGAGGATTCATCGACGTGACCAGCACCAAAGGCCAAGGCGCCACCATCATCGTGCGCTTGCCAGCAGCGCAGTAA

Upstream 100 bases:

>100_bases
GGCCCTGCGCCGATCCTCCATCGAAAACATGCGGACTCCAATCCGGACGCCCAAGCGTCCAACTTTCCGTCCGCATCCCC
TTCGGAATTCGACTGATCAG

Downstream 100 bases:

>100_bases
GGCGCCATCAGCGAAGTTACTTGCGAATCCGGAATGGGGCCGCAGGCAGGCCGAAGGCGTTGAACAGCGGTGCCGGGCCC
CAACTGAACCAGCCGTAACG

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MFADFNTMVEELGGIETMKNDFVSNVSHEIRNPLAAIKNYATMMRDMDMDKAQREECVRTIISASERLNALVTNILKLNK
LESQTIVSQAVDYDLTRQLTDEILTLDDLFTEKNIDLGCDLEDRAMVHADLGITTLIWSNILGNALKYTESGGHVSFTQR
SHGNMVSVEISDDGCDMTPDEAAHMFDKFYQGDTSHAAQGNGLGMAMVKRAVDLSRGFIDVTSTKGQGATIIVRLPAAQ

Sequences:

>Translated_239_residues
MFADFNTMVEELGGIETMKNDFVSNVSHEIRNPLAAIKNYATMMRDMDMDKAQREECVRTIISASERLNALVTNILKLNK
LESQTIVSQAVDYDLTRQLTDEILTLDDLFTEKNIDLGCDLEDRAMVHADLGITTLIWSNILGNALKYTESGGHVSFTQR
SHGNMVSVEISDDGCDMTPDEAAHMFDKFYQGDTSHAAQGNGLGMAMVKRAVDLSRGFIDVTSTKGQGATIIVRLPAAQ
>Mature_239_residues
MFADFNTMVEELGGIETMKNDFVSNVSHEIRNPLAAIKNYATMMRDMDMDKAQREECVRTIISASERLNALVTNILKLNK
LESQTIVSQAVDYDLTRQLTDEILTLDDLFTEKNIDLGCDLEDRAMVHADLGITTLIWSNILGNALKYTESGGHVSFTQR
SHGNMVSVEISDDGCDMTPDEAAHMFDKFYQGDTSHAAQGNGLGMAMVKRAVDLSRGFIDVTSTKGQGATIIVRLPAAQ

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=235, Percent_Identity=28.0851063829787, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786783, Length=238, Percent_Identity=25.6302521008403, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788393, Length=235, Percent_Identity=25.9574468085106, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI145693157, Length=223, Percent_Identity=28.2511210762332, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788713, Length=224, Percent_Identity=25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=224, Percent_Identity=26.7857142857143, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=26.7543859649123, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788549, Length=218, Percent_Identity=24.3119266055046, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87082128, Length=237, Percent_Identity=21.9409282700422, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790346, Length=240, Percent_Identity=23.75, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=222, Percent_Identity=28.8288288288288, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=26.8518518518519, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790861, Length=229, Percent_Identity=26.2008733624454, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1789808, Length=240, Percent_Identity=24.5833333333333, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786912, Length=235, Percent_Identity=24.2553191489362, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=234, Percent_Identity=23.0769230769231, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788279, Length=200, Percent_Identity=24.5, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 26375; Mature: 26375

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFADFNTMVEELGGIETMKNDFVSNVSHEIRNPLAAIKNYATMMRDMDMDKAQREECVRT
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IISASERLNALVTNILKLNKLESQTIVSQAVDYDLTRQLTDEILTLDDLFTEKNIDLGCD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LEDRAMVHADLGITTLIWSNILGNALKYTESGGHVSFTQRSHGNMVSVEISDDGCDMTPD
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHH
EAAHMFDKFYQGDTSHAAQGNGLGMAMVKRAVDLSRGFIDVTSTKGQGATIIVRLPAAQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCC
>Mature Secondary Structure
MFADFNTMVEELGGIETMKNDFVSNVSHEIRNPLAAIKNYATMMRDMDMDKAQREECVRT
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IISASERLNALVTNILKLNKLESQTIVSQAVDYDLTRQLTDEILTLDDLFTEKNIDLGCD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LEDRAMVHADLGITTLIWSNILGNALKYTESGGHVSFTQRSHGNMVSVEISDDGCDMTPD
CCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCHH
EAAHMFDKFYQGDTSHAAQGNGLGMAMVKRAVDLSRGFIDVTSTKGQGATIIVRLPAAQ
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]