Click here to switch to the map view.

The map label for this gene is ppnK [H]

Identifier: 213692728

GI number: 213692728

Start: 2031144

End: 2032160

Strand: Reverse

Name: ppnK [H]

Synonym: Blon_1862

Alternate gene names: 213692728

Gene position: 2032160-2031144 (Counterclockwise)

Preceding gene: 213692730

Following gene: 213692727

Centisome position: 71.74

GC content: 65.49

Gene sequence:

>1017_bases
ATGGCCAAGCGCAACGCGGTGGTGGTGACGCATACCAGGCTCAGGCAGACCGGAACCGTAGTGGCCGAGGCGGTGAGCCA
GCTGCGAGTGGCCGGTTTCGAAGTCACCATCATCGACAACACCGAGGCCCCCGATTTCGGCGTGCAGCCGCCGTGCGTGT
CGGACGACACCGAAATCGTCGTGGTCCTGGGCGGCGACGGCACGATACTGCGCGCCGCCGAACTCGTCCACTGCACCCAG
GTGCCGATTCTTGGCGTGAACATGGGGCATGTCGGCTTCCTGGCCGAATTCGAAAGCTTCCAGATCGACGAGGCGATCCG
CCGGGTCGCCACCCACGACTATTCGATCGACGAACGCATGATCGCCCATGTGGACGTCTGGCTGCCTGGCGCCACCAAGC
CCATTGAGGATTGGGCGCTGAACGACATCACGCTGGAACGCGCCGACCGGGGCAAGATGGTCGAACTGTCCATCCGCGTG
GACGATGTGGAGATGAACTCGTTCGGCGCGGACGGCGTCATCGTCTCCACGCCCACCGGTTCCACCGCCTACGCCTTCTC
CGCGGGCGGTCCGGTCATGTGGCCGAACGTCAAGGCCCTGCAGTTGATTCCGTTGGCCGCGCACGCCCTGTTCGCCAGGC
CGCTGATCATCGGCTCCGGTTCGACCTTTACCATCGATATTCTGGACGATTCCATGTCCGAAGGCTGGATTTGCTGCGAT
GGACGCCGTCAGCGCGCCCTGCCGCAGGGTACGCGCGTCATGGTGCGCGAATCGCGCGATACCTTGCGCCTCGCTCGACT
CTCCGGTATGCCGTTCACCAACCGTCTGGTCTCCAAGTTCGATCTGCCCGTGGTCGGTTGGCGCGAGCACGCGCGCAACG
AGGCCTCGAGCCAGCCGCTGCATCACGGGCACACGTTCCCGGCGGCCGCGTATGCGGCTGGCGTTGCCGGTGACGCTGGT
GTCGCTGGTACCGAGCCCGACAAGCCGGGCGAACGCGACGGCAAGGCAGGAGCCTGA

Upstream 100 bases:

>100_bases
GACGCGCCACGAAGCAGCACTTTTCGAACACCTGTACGACTGATGACGGTGTCTGCGGCTAGAATAATGAGCAGTGCGAC
CGAATCGACAGGAGGATGCC

Downstream 100 bases:

>100_bases
TGCTCGAAGAACTCGATATCCGCAATCTGGGGCCGATTCGAGAAGCCACCATTGCGCCGGCCGCAGGCATGACCGCCATC
ACCGGTGAGACCGGCGCCGG

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAKRNAVVVTHTRLRQTGTVVAEAVSQLRVAGFEVTIIDNTEAPDFGVQPPCVSDDTEIVVVLGGDGTILRAAELVHCTQ
VPILGVNMGHVGFLAEFESFQIDEAIRRVATHDYSIDERMIAHVDVWLPGATKPIEDWALNDITLERADRGKMVELSIRV
DDVEMNSFGADGVIVSTPTGSTAYAFSAGGPVMWPNVKALQLIPLAAHALFARPLIIGSGSTFTIDILDDSMSEGWICCD
GRRQRALPQGTRVMVRESRDTLRLARLSGMPFTNRLVSKFDLPVVGWREHARNEASSQPLHHGHTFPAAAYAAGVAGDAG
VAGTEPDKPGERDGKAGA

Sequences:

>Translated_338_residues
MAKRNAVVVTHTRLRQTGTVVAEAVSQLRVAGFEVTIIDNTEAPDFGVQPPCVSDDTEIVVVLGGDGTILRAAELVHCTQ
VPILGVNMGHVGFLAEFESFQIDEAIRRVATHDYSIDERMIAHVDVWLPGATKPIEDWALNDITLERADRGKMVELSIRV
DDVEMNSFGADGVIVSTPTGSTAYAFSAGGPVMWPNVKALQLIPLAAHALFARPLIIGSGSTFTIDILDDSMSEGWICCD
GRRQRALPQGTRVMVRESRDTLRLARLSGMPFTNRLVSKFDLPVVGWREHARNEASSQPLHHGHTFPAAAYAAGVAGDAG
VAGTEPDKPGERDGKAGA
>Mature_337_residues
AKRNAVVVTHTRLRQTGTVVAEAVSQLRVAGFEVTIIDNTEAPDFGVQPPCVSDDTEIVVVLGGDGTILRAAELVHCTQV
PILGVNMGHVGFLAEFESFQIDEAIRRVATHDYSIDERMIAHVDVWLPGATKPIEDWALNDITLERADRGKMVELSIRVD
DVEMNSFGADGVIVSTPTGSTAYAFSAGGPVMWPNVKALQLIPLAAHALFARPLIIGSGSTFTIDILDDSMSEGWICCDG
RRQRALPQGTRVMVRESRDTLRLARLSGMPFTNRLVSKFDLPVVGWREHARNEASSQPLHHGHTFPAAAYAAGVAGDAGV
AGTEPDKPGERDGKAGA

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=232, Percent_Identity=26.2931034482759, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788968, Length=232, Percent_Identity=31.0344827586207, Blast_Score=125, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320794, Length=217, Percent_Identity=30.4147465437788, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322509, Length=212, Percent_Identity=29.2452830188679, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6325068, Length=210, Percent_Identity=32.3809523809524, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI161077047, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573828, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573830, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573832, Length=223, Percent_Identity=28.6995515695067, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28573826, Length=223, Percent_Identity=28.6995515695067, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20129957, Length=248, Percent_Identity=26.2096774193548, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281363321, Length=248, Percent_Identity=25.4032258064516, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281363323, Length=248, Percent_Identity=25.4032258064516, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24653422, Length=248, Percent_Identity=26.2096774193548, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653424, Length=248, Percent_Identity=25.4032258064516, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 36343; Mature: 36211

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRNAVVVTHTRLRQTGTVVAEAVSQLRVAGFEVTIIDNTEAPDFGVQPPCVSDDTEIV
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCEEE
VVLGGDGTILRAAELVHCTQVPILGVNMGHVGFLAEFESFQIDEAIRRVATHDYSIDERM
EEECCCCCEEHHHHHHHHCCCCEEEECCCCEEEEHHHHCCCHHHHHHHHHHCCCCCCCEE
IAHVDVWLPGATKPIEDWALNDITLERADRGKMVELSIRVDDVEMNSFGADGVIVSTPTG
EEEEEEECCCCCCCCHHHCCCCEEEEECCCCCEEEEEEEEEEEEECCCCCCCEEEECCCC
STAYAFSAGGPVMWPNVKALQLIPLAAHALFARPLIIGSGSTFTIDILDDSMSEGWICCD
CEEEEEECCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCEEEEEEECCCCCCCEEEEC
GRRQRALPQGTRVMVRESRDTLRLARLSGMPFTNRLVSKFDLPVVGWREHARNEASSQPL
CCCCCCCCCCCEEEEECCCCCEEEEHHCCCCHHHHHHHHCCCCEECHHHHHCCCCCCCCC
HHGHTFPAAAYAAGVAGDAGVAGTEPDKPGERDGKAGA
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKRNAVVVTHTRLRQTGTVVAEAVSQLRVAGFEVTIIDNTEAPDFGVQPPCVSDDTEIV
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCEEE
VVLGGDGTILRAAELVHCTQVPILGVNMGHVGFLAEFESFQIDEAIRRVATHDYSIDERM
EEECCCCCEEHHHHHHHHCCCCEEEECCCCEEEEHHHHCCCHHHHHHHHHHCCCCCCCEE
IAHVDVWLPGATKPIEDWALNDITLERADRGKMVELSIRVDDVEMNSFGADGVIVSTPTG
EEEEEEECCCCCCCCHHHCCCCEEEEECCCCCEEEEEEEEEEEEECCCCCCCEEEECCCC
STAYAFSAGGPVMWPNVKALQLIPLAAHALFARPLIIGSGSTFTIDILDDSMSEGWICCD
CEEEEEECCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCEEEEEEECCCCCCCEEEEC
GRRQRALPQGTRVMVRESRDTLRLARLSGMPFTNRLVSKFDLPVVGWREHARNEASSQPL
CCCCCCCCCCCEEEEECCCCCEEEEHHCCCCHHHHHHHHCCCCEECHHHHHCCCCCCCCC
HHGHTFPAAAYAAGVAGDAGVAGTEPDKPGERDGKAGA
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]