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The map label for this gene is pyk [H]

Identifier: 213692612

GI number: 213692612

Start: 1916553

End: 1917995

Strand: Reverse

Name: pyk [H]

Synonym: Blon_1745

Alternate gene names: 213692612

Gene position: 1917995-1916553 (Counterclockwise)

Preceding gene: 213692613

Following gene: 213692611

Centisome position: 67.71

GC content: 63.69

Gene sequence:

>1443_bases
ATGCGTAAAGCCAAGATCGTAGACACCATCGGTCCTTCCACCGAGGATTACGACAACCTCCTGAAGCTCGTTGAAGCCGG
TATGGACGTTGCTCGTCTGAACCGCTCCCACGGCACTCCCGAGGATCACCTCAAGGTCTACAACAACGTGCGCAAGGCCA
GCGAAGCCACTGGTCGCAACGTTGCCGCGCTTGTTGATTTGCAGGGCCCGAAGATCCGTTGCGGTTGGTTCAAGAAGAAC
GCCGACGGCGAAGACAAGGTTCAGCTGCAGCTCGGCCAGGAATTCGTCATCACCACTGATGACGTCGAGGGCGACGAGCA
TATCACCTCCACCACCTTCAAGGGCCTGCCGGGCGATTGCCATCCGGGCGACCCGATCCTGATCGATGACGGCAAGGTCC
GTCTTGAGGTCACCAAGGTCGAGGGCAACAACGTGTACACCAAGGTCGTCGTGGCCGGCCCCGTGTCCTCCCACAAGGGC
ATCAACCTGCCGGGCGTCGCCGTCTCCCTGCCGGCCCTGACCGAGAAGGATGAGGCCGATCTGCGTTGGGCCATCCGCAC
CGGTGCCGACATCATCGCCATGTCCTTCGTGCGTTTCGCCACCGACATCGACCGCGCCCACGAGATCATGGACGAGGAAG
GCCGCCGCATCCCGATCGTCGCCAAGATCGAGAAGCCGCAGGCTCTGGAGAACCTCGAAGAGATCGTCAAGACCTTCGAC
GGTGTCATGGCCGCCCGTGGCGACATGGCCGTTGAGTGCCCGCTGGAAGAGGTCCCGCTGGCCACCAAGCGCATCATCGA
GCTGGCCCGCCAGTACGCCAAGCCGGTCATCGTGGCCACCGAAGTCCTCGGCTCCATGGTGAACTCCCCGGTTCCGACCC
GTGCAGAGGCTTCCGACTGCGCCAACGCCGTCCTCGACGGCTCCGACGCCACCATGACCTCCAACGAGACCGCCGTCGGC
AAGTACCCGGATGTGACCGTGGCCACCATGTCGCGTATCTCCGGCTACGCCACCGACCACGGCTTCGACCGCATCCCCGA
GCTCAAGAACCTCGACATGTCCTCCACCGGCGCCGTTTCCTCCGCCGCCGTGGACCTGGCCGACAAGCTCAACGCCAAGG
CCATCGTCGCCTACACCCAGACCGGCGCCACCGTCCACCGCGTCTCCCGTGAGCGTCCGGCCGCCCCGATCTATGGTCTG
ACCAGCAACGAGCACACCTACCACTGGCTGGCTCTGTCCTGGGGCACCGAAGCCTTCCTGCTCAAGGAGGACTACCACGA
CAAGTCCCGCAAGGATCTGATGATCTACACGGACAAGATCCTCAAGGACGCCGGCAAGGTCGTCGACGGCGACAAGATCG
TCATCCTGAGCTCCGCCCAGGGCGATCACCAGCCGGGCCGCACCGACTCCATCTACGTGCACACCGTTGGCGCCTGCGAC
TGA

Upstream 100 bases:

>100_bases
AAGAAAACTTGTCATGAGTCGGCTCGCGCTAGTAGACTGAGTTTCGGCAAGTGAGGCGATCGCGCCTCGAACCAAATTGA
ACAAGAATAGGCAGGCATTC

Downstream 100 bases:

>100_bases
TGTCGTGTAGCCGCTGACCGCTGATGTGCGGTACGTAAAGGCCCTCATCCCGCTGCGGGGTGGGGGCCTTCGCGTATCGG
ACGGCGGCATCGCCGGGTGC

Product: Pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MRKAKIVDTIGPSTEDYDNLLKLVEAGMDVARLNRSHGTPEDHLKVYNNVRKASEATGRNVAALVDLQGPKIRCGWFKKN
ADGEDKVQLQLGQEFVITTDDVEGDEHITSTTFKGLPGDCHPGDPILIDDGKVRLEVTKVEGNNVYTKVVVAGPVSSHKG
INLPGVAVSLPALTEKDEADLRWAIRTGADIIAMSFVRFATDIDRAHEIMDEEGRRIPIVAKIEKPQALENLEEIVKTFD
GVMAARGDMAVECPLEEVPLATKRIIELARQYAKPVIVATEVLGSMVNSPVPTRAEASDCANAVLDGSDATMTSNETAVG
KYPDVTVATMSRISGYATDHGFDRIPELKNLDMSSTGAVSSAAVDLADKLNAKAIVAYTQTGATVHRVSRERPAAPIYGL
TSNEHTYHWLALSWGTEAFLLKEDYHDKSRKDLMIYTDKILKDAGKVVDGDKIVILSSAQGDHQPGRTDSIYVHTVGACD

Sequences:

>Translated_480_residues
MRKAKIVDTIGPSTEDYDNLLKLVEAGMDVARLNRSHGTPEDHLKVYNNVRKASEATGRNVAALVDLQGPKIRCGWFKKN
ADGEDKVQLQLGQEFVITTDDVEGDEHITSTTFKGLPGDCHPGDPILIDDGKVRLEVTKVEGNNVYTKVVVAGPVSSHKG
INLPGVAVSLPALTEKDEADLRWAIRTGADIIAMSFVRFATDIDRAHEIMDEEGRRIPIVAKIEKPQALENLEEIVKTFD
GVMAARGDMAVECPLEEVPLATKRIIELARQYAKPVIVATEVLGSMVNSPVPTRAEASDCANAVLDGSDATMTSNETAVG
KYPDVTVATMSRISGYATDHGFDRIPELKNLDMSSTGAVSSAAVDLADKLNAKAIVAYTQTGATVHRVSRERPAAPIYGL
TSNEHTYHWLALSWGTEAFLLKEDYHDKSRKDLMIYTDKILKDAGKVVDGDKIVILSSAQGDHQPGRTDSIYVHTVGACD
>Mature_480_residues
MRKAKIVDTIGPSTEDYDNLLKLVEAGMDVARLNRSHGTPEDHLKVYNNVRKASEATGRNVAALVDLQGPKIRCGWFKKN
ADGEDKVQLQLGQEFVITTDDVEGDEHITSTTFKGLPGDCHPGDPILIDDGKVRLEVTKVEGNNVYTKVVVAGPVSSHKG
INLPGVAVSLPALTEKDEADLRWAIRTGADIIAMSFVRFATDIDRAHEIMDEEGRRIPIVAKIEKPQALENLEEIVKTFD
GVMAARGDMAVECPLEEVPLATKRIIELARQYAKPVIVATEVLGSMVNSPVPTRAEASDCANAVLDGSDATMTSNETAVG
KYPDVTVATMSRISGYATDHGFDRIPELKNLDMSSTGAVSSAAVDLADKLNAKAIVAYTQTGATVHRVSRERPAAPIYGL
TSNEHTYHWLALSWGTEAFLLKEDYHDKSRKDLMIYTDKILKDAGKVVDGDKIVILSSAQGDHQPGRTDSIYVHTVGACD

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=473, Percent_Identity=36.1522198731501, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI33286422, Length=473, Percent_Identity=36.5750528541226, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI33286420, Length=473, Percent_Identity=36.5750528541226, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI10835121, Length=436, Percent_Identity=36.697247706422, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI32967597, Length=436, Percent_Identity=36.697247706422, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=37.7162629757785, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=37.7162629757785, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=36.2869198312236, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=36.2869198312236, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=36.5740740740741, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1787965, Length=477, Percent_Identity=41.0901467505241, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1788160, Length=493, Percent_Identity=38.5395537525355, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=442, Percent_Identity=39.5927601809955, Blast_Score=277, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI71984406, Length=427, Percent_Identity=37.7049180327869, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71984413, Length=427, Percent_Identity=37.7049180327869, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17506829, Length=427, Percent_Identity=37.7049180327869, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17506831, Length=427, Percent_Identity=37.7049180327869, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319279, Length=492, Percent_Identity=35.3658536585366, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=33.6734693877551, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24648964, Length=495, Percent_Identity=35.959595959596, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI28571814, Length=495, Percent_Identity=35.959595959596, Blast_Score=265, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24648966, Length=415, Percent_Identity=38.0722891566265, Blast_Score=254, Evalue=8e-68,
Organism=Drosophila melanogaster, GI24581235, Length=475, Percent_Identity=30.5263157894737, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24646914, Length=279, Percent_Identity=38.3512544802867, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52101; Mature: 52101

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAKIVDTIGPSTEDYDNLLKLVEAGMDVARLNRSHGTPEDHLKVYNNVRKASEATGRN
CCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
VAALVDLQGPKIRCGWFKKNADGEDKVQLQLGQEFVITTDDVEGDEHITSTTFKGLPGDC
EEEEEECCCCEEEEEEEECCCCCCCEEEEEECCEEEEEECCCCCCCCEEHHCCCCCCCCC
HPGDPILIDDGKVRLEVTKVEGNNVYTKVVVAGPVSSHKGINLPGVAVSLPALTEKDEAD
CCCCCEEECCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCC
LRWAIRTGADIIAMSFVRFATDIDRAHEIMDEEGRRIPIVAKIEKPQALENLEEIVKTFD
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHH
GVMAARGDMAVECPLEEVPLATKRIIELARQYAKPVIVATEVLGSMVNSPVPTRAEASDC
HHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHH
ANAVLDGSDATMTSNETAVGKYPDVTVATMSRISGYATDHGFDRIPELKNLDMSSTGAVS
HHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCH
SAAVDLADKLNAKAIVAYTQTGATVHRVSRERPAAPIYGLTSNEHTYHWLALSWGTEAFL
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCCEEEE
LKEDYHDKSRKDLMIYTDKILKDAGKVVDGDKIVILSSAQGDHQPGRTDSIYVHTVGACD
EECCCCCCCCCCEEEEHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MRKAKIVDTIGPSTEDYDNLLKLVEAGMDVARLNRSHGTPEDHLKVYNNVRKASEATGRN
CCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
VAALVDLQGPKIRCGWFKKNADGEDKVQLQLGQEFVITTDDVEGDEHITSTTFKGLPGDC
EEEEEECCCCEEEEEEEECCCCCCCEEEEEECCEEEEEECCCCCCCCEEHHCCCCCCCCC
HPGDPILIDDGKVRLEVTKVEGNNVYTKVVVAGPVSSHKGINLPGVAVSLPALTEKDEAD
CCCCCEEECCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCC
LRWAIRTGADIIAMSFVRFATDIDRAHEIMDEEGRRIPIVAKIEKPQALENLEEIVKTFD
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHH
GVMAARGDMAVECPLEEVPLATKRIIELARQYAKPVIVATEVLGSMVNSPVPTRAEASDC
HHHHCCCCEEEECCHHHCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHH
ANAVLDGSDATMTSNETAVGKYPDVTVATMSRISGYATDHGFDRIPELKNLDMSSTGAVS
HHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCH
SAAVDLADKLNAKAIVAYTQTGATVHRVSRERPAAPIYGLTSNEHTYHWLALSWGTEAFL
HHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCCCCEEECCCCCCEEEEEEEECCCEEEE
LKEDYHDKSRKDLMIYTDKILKDAGKVVDGDKIVILSSAQGDHQPGRTDSIYVHTVGACD
EECCCCCCCCCCEEEEHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]