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The map label for this gene is glgX [H]

Identifier: 213692607

GI number: 213692607

Start: 1908407

End: 1910548

Strand: Reverse

Name: glgX [H]

Synonym: Blon_1740

Alternate gene names: 213692607

Gene position: 1910548-1908407 (Counterclockwise)

Preceding gene: 213692608

Following gene: 213692606

Centisome position: 67.45

GC content: 59.1

Gene sequence:

>2142_bases
ATGCAGATCAGACCTGGATCAATGTATCCGCTGGGCGCCAGCTATGACGGCGCCGGTGTGAACTTCGCGCTGTACTCACA
GGTTGCGCAGAAAGTCGAGCTATGCCTGTTCGACGAGCACGATGCGGAAACGCGCATCGAAATGACCGAACAGAACTCAT
ACGTATGGCATAACTACATCCCCGGCCTCCAGCCCGGCCAACGCTACGGCTACCGCGTCTACGGTCCCTATGACCCGATG
CACGGCCTCAGATGCAACCCCAACAAACTGCTGCTCGACCCGTATGCCAAGGCCATCGAAGGCAACATCGACGGCGACGA
AAGCCTGTTCTCCTACTGGTTCAAAAGCCCGGACGACACCTCGGCGATGAACGACCTTGACTCGGCCGCCCACACCATGA
AGTCGGCCGTCATCAATCCGTATTTCGACTGGGGCAACGACCAGCACCCCTACATCTCGTACCACGATTCGGTGATCTAC
GAGGCGCACGTGCGCGGCATGACCAACCTCAACATGGACGTGCCGCCGGACATCCGCGGCACCTACGCCGGCCTGGCCTA
CCCGAGCGTCATCGAATACCTGAAGAAGCTCGGCATCACCGCCATCGAACTCATGCCGATCCACCAGTTCGTCAACGACT
CCTTCCTGCAGGAGAAGGGCCTGTCGAACTACTGGGGCTACAACACCATCGGCTTCTTCGCGCCGCACAACGCCTACTCC
TCCTCCGGGGAACGCGGCGAGCAGGTCAACGAATTCAAGTCCATGGTCAAGGCCTACCATCACGCCGGCATGGAAGTCAT
CCTCGACGTGGTGTACAACCACACCGCCGAAGGTAACCATATGGGCCCCACCCTGAGCTTCAAGGGCATCGACAACGCCT
CCTACTACCGACTGGTGGAAGGCGACCAGCAGCACTACTTCGACACCACCGGTACCGGCAACTCACTGCTCATGCGCTCG
CCGCACGCGCTGCAGTTGATTACCGATTCGCTGCGCTACTGGGTTACCGAAATGCACGTCGACGGCTTCCGCTTCGATCT
GGCCGCCACGCTGGCACGCCAGTTCCAGGAGGTCGACAAGCTGTCTGCGTTCTTCGACATCGTGGAGCAGGACCCGATCA
TCTCGCGCGTGAAGCTGATTGCCGAACCGTGGGATCTGGGCTCCGGCGGCTATCAGGTGGGTGGCTTCCCGTCCAGCTGG
TCCGAATGGAATGGCCGCTACCGCGACACGGTGCGCGACTTCTGGCGCTCGCAGCCCTCCACGTTGCCCGAATTCGCATC
ACGCCTCATGGGCAGCTCCGATCTGTATCAGGTCAACGGCCGTCGACCGGTGGCGTCCGTGAACTTCATCACCGCGCATG
ACGGTTTCACGATGAACGACCTGGTCAGCTACAACGAAAAGCACAACGAAGCCAATGGCGAGGGCAATCGCGACGGCGAG
AGCAACAATCGCTCGTGGAATTGCGGCGTTGAAGGCCCGACCAATATTCCGGACGTCAACGACTTGCGTCAGCGTCAGAT
GCGCAACATGTTCGCCACGTTGCTGTTCAGCCAGGGTATCCCGATGATTTGCGGCGGCGACGAAGTGGCCCGCACCCAGC
AGGGCAACAACAACGCCTACTGTCAGGACAACGAGATCTCCTGGACCAACTGGCATCTTGACAAGGGACGCAAGGAACTG
CTCGCCTTCGTTTCCAAACTCATTCATCTACGTTTGGATCATCCGGTGTTGCACCGCCGTCGCTTCTTCACAGGCCGTGA
GCCCGGCGACGATTCGAACATGATTCCGCAGGTCGAATGGTTCGACCACACCGGTTCGATCATGGATATGGACGACTGGC
AGAACACGCATGCGTTCTCCATGATGATCTATCTGAACGGTTCGGATATTCCGGAGGTAGACTGGTACGGCAACCGGATG
GTCGACAACGACTTCATCCTGATCTTCAACGCGCATTACGAACCGATTATGTTCACCCTGCCGGATGAGCGATACGGCCG
CAAATGGCAGCTGGTCGTGGATACGCACAACCCGGATGGGCCGGAACTGAGCTATGAGGCGGGCTTCATGATCACCGCCC
AGTCCCGCAGCTTCCTCATGCTCATGAGCGACAAAAAGCCCAAGAAGCCCATGGGGGCGTGA

Upstream 100 bases:

>100_bases
CTCATCGCCAGGAACTCCGACAAAGACAAGCCGTCGCTGTTCTCCTGACGTTCAACTCCCCGGCACATCAGTGACACAAG
AAAGTGGGATACTGAAAACT

Downstream 100 bases:

>100_bases
CGCCGGCGGGAGCGATGCGGGCTGGCGGGATCGGACTGGGCCGGTTCGGGTTGGTTCGGACTGTTCCGGGTTGGTCTGTG
CCGTTCCAGATTGGCTGGCT

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MQIRPGSMYPLGASYDGAGVNFALYSQVAQKVELCLFDEHDAETRIEMTEQNSYVWHNYIPGLQPGQRYGYRVYGPYDPM
HGLRCNPNKLLLDPYAKAIEGNIDGDESLFSYWFKSPDDTSAMNDLDSAAHTMKSAVINPYFDWGNDQHPYISYHDSVIY
EAHVRGMTNLNMDVPPDIRGTYAGLAYPSVIEYLKKLGITAIELMPIHQFVNDSFLQEKGLSNYWGYNTIGFFAPHNAYS
SSGERGEQVNEFKSMVKAYHHAGMEVILDVVYNHTAEGNHMGPTLSFKGIDNASYYRLVEGDQQHYFDTTGTGNSLLMRS
PHALQLITDSLRYWVTEMHVDGFRFDLAATLARQFQEVDKLSAFFDIVEQDPIISRVKLIAEPWDLGSGGYQVGGFPSSW
SEWNGRYRDTVRDFWRSQPSTLPEFASRLMGSSDLYQVNGRRPVASVNFITAHDGFTMNDLVSYNEKHNEANGEGNRDGE
SNNRSWNCGVEGPTNIPDVNDLRQRQMRNMFATLLFSQGIPMICGGDEVARTQQGNNNAYCQDNEISWTNWHLDKGRKEL
LAFVSKLIHLRLDHPVLHRRRFFTGREPGDDSNMIPQVEWFDHTGSIMDMDDWQNTHAFSMMIYLNGSDIPEVDWYGNRM
VDNDFILIFNAHYEPIMFTLPDERYGRKWQLVVDTHNPDGPELSYEAGFMITAQSRSFLMLMSDKKPKKPMGA

Sequences:

>Translated_713_residues
MQIRPGSMYPLGASYDGAGVNFALYSQVAQKVELCLFDEHDAETRIEMTEQNSYVWHNYIPGLQPGQRYGYRVYGPYDPM
HGLRCNPNKLLLDPYAKAIEGNIDGDESLFSYWFKSPDDTSAMNDLDSAAHTMKSAVINPYFDWGNDQHPYISYHDSVIY
EAHVRGMTNLNMDVPPDIRGTYAGLAYPSVIEYLKKLGITAIELMPIHQFVNDSFLQEKGLSNYWGYNTIGFFAPHNAYS
SSGERGEQVNEFKSMVKAYHHAGMEVILDVVYNHTAEGNHMGPTLSFKGIDNASYYRLVEGDQQHYFDTTGTGNSLLMRS
PHALQLITDSLRYWVTEMHVDGFRFDLAATLARQFQEVDKLSAFFDIVEQDPIISRVKLIAEPWDLGSGGYQVGGFPSSW
SEWNGRYRDTVRDFWRSQPSTLPEFASRLMGSSDLYQVNGRRPVASVNFITAHDGFTMNDLVSYNEKHNEANGEGNRDGE
SNNRSWNCGVEGPTNIPDVNDLRQRQMRNMFATLLFSQGIPMICGGDEVARTQQGNNNAYCQDNEISWTNWHLDKGRKEL
LAFVSKLIHLRLDHPVLHRRRFFTGREPGDDSNMIPQVEWFDHTGSIMDMDDWQNTHAFSMMIYLNGSDIPEVDWYGNRM
VDNDFILIFNAHYEPIMFTLPDERYGRKWQLVVDTHNPDGPELSYEAGFMITAQSRSFLMLMSDKKPKKPMGA
>Mature_713_residues
MQIRPGSMYPLGASYDGAGVNFALYSQVAQKVELCLFDEHDAETRIEMTEQNSYVWHNYIPGLQPGQRYGYRVYGPYDPM
HGLRCNPNKLLLDPYAKAIEGNIDGDESLFSYWFKSPDDTSAMNDLDSAAHTMKSAVINPYFDWGNDQHPYISYHDSVIY
EAHVRGMTNLNMDVPPDIRGTYAGLAYPSVIEYLKKLGITAIELMPIHQFVNDSFLQEKGLSNYWGYNTIGFFAPHNAYS
SSGERGEQVNEFKSMVKAYHHAGMEVILDVVYNHTAEGNHMGPTLSFKGIDNASYYRLVEGDQQHYFDTTGTGNSLLMRS
PHALQLITDSLRYWVTEMHVDGFRFDLAATLARQFQEVDKLSAFFDIVEQDPIISRVKLIAEPWDLGSGGYQVGGFPSSW
SEWNGRYRDTVRDFWRSQPSTLPEFASRLMGSSDLYQVNGRRPVASVNFITAHDGFTMNDLVSYNEKHNEANGEGNRDGE
SNNRSWNCGVEGPTNIPDVNDLRQRQMRNMFATLLFSQGIPMICGGDEVARTQQGNNNAYCQDNEISWTNWHLDKGRKEL
LAFVSKLIHLRLDHPVLHRRRFFTGREPGDDSNMIPQVEWFDHTGSIMDMDDWQNTHAFSMMIYLNGSDIPEVDWYGNRM
VDNDFILIFNAHYEPIMFTLPDERYGRKWQLVVDTHNPDGPELSYEAGFMITAQSRSFLMLMSDKKPKKPMGA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=615, Percent_Identity=46.1788617886179, Blast_Score=544, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 81429; Mature: 81429

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRPGSMYPLGASYDGAGVNFALYSQVAQKVELCLFDEHDAETRIEMTEQNSYVWHNYI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCEEEECCC
PGLQPGQRYGYRVYGPYDPMHGLRCNPNKLLLDPYAKAIEGNIDGDESLFSYWFKSPDDT
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHHHCCCCCCHHHHHHHHCCCCCCC
SAMNDLDSAAHTMKSAVINPYFDWGNDQHPYISYHDSVIYEAHVRGMTNLNMDVPPDIRG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEEHHCCCCCCCCCCCCCCCC
TYAGLAYPSVIEYLKKLGITAIELMPIHQFVNDSFLQEKGLSNYWGYNTIGFFAPHNAYS
CCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
SSGERGEQVNEFKSMVKAYHHAGMEVILDVVYNHTAEGNHMGPTLSFKGIDNASYYRLVE
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEEEC
GDQQHYFDTTGTGNSLLMRSPHALQLITDSLRYWVTEMHVDGFRFDLAATLARQFQEVDK
CCCHHEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LSAFFDIVEQDPIISRVKLIAEPWDLGSGGYQVGGFPSSWSEWNGRYRDTVRDFWRSQPS
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHCCCHHHHHHHHHCCCCC
TLPEFASRLMGSSDLYQVNGRRPVASVNFITAHDGFTMNDLVSYNEKHNEANGEGNRDGE
CHHHHHHHHCCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHCHHCCCCCCCCCCCCC
SNNRSWNCGVEGPTNIPDVNDLRQRQMRNMFATLLFSQGIPMICGGDEVARTQQGNNNAY
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCC
CQDNEISWTNWHLDKGRKELLAFVSKLIHLRLDHPVLHRRRFFTGREPGDDSNMIPQVEW
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEE
FDHTGSIMDMDDWQNTHAFSMMIYLNGSDIPEVDWYGNRMVDNDFILIFNAHYEPIMFTL
ECCCCCEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCEEEEC
PDERYGRKWQLVVDTHNPDGPELSYEAGFMITAQSRSFLMLMSDKKPKKPMGA
CCHHHCCEEEEEEECCCCCCCCEEECCCEEEEECCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MQIRPGSMYPLGASYDGAGVNFALYSQVAQKVELCLFDEHDAETRIEMTEQNSYVWHNYI
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCEEEECCC
PGLQPGQRYGYRVYGPYDPMHGLRCNPNKLLLDPYAKAIEGNIDGDESLFSYWFKSPDDT
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEECCHHHHHCCCCCCHHHHHHHHCCCCCCC
SAMNDLDSAAHTMKSAVINPYFDWGNDQHPYISYHDSVIYEAHVRGMTNLNMDVPPDIRG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEEEEHHCCCCCCCCCCCCCCCC
TYAGLAYPSVIEYLKKLGITAIELMPIHQFVNDSFLQEKGLSNYWGYNTIGFFAPHNAYS
CCCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
SSGERGEQVNEFKSMVKAYHHAGMEVILDVVYNHTAEGNHMGPTLSFKGIDNASYYRLVE
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCEEEEEC
GDQQHYFDTTGTGNSLLMRSPHALQLITDSLRYWVTEMHVDGFRFDLAATLARQFQEVDK
CCCHHEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
LSAFFDIVEQDPIISRVKLIAEPWDLGSGGYQVGGFPSSWSEWNGRYRDTVRDFWRSQPS
HHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHCCCHHHHHHHHHCCCCC
TLPEFASRLMGSSDLYQVNGRRPVASVNFITAHDGFTMNDLVSYNEKHNEANGEGNRDGE
CHHHHHHHHCCCCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHCHHCCCCCCCCCCCCC
SNNRSWNCGVEGPTNIPDVNDLRQRQMRNMFATLLFSQGIPMICGGDEVARTQQGNNNAY
CCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCC
CQDNEISWTNWHLDKGRKELLAFVSKLIHLRLDHPVLHRRRFFTGREPGDDSNMIPQVEW
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCEEE
FDHTGSIMDMDDWQNTHAFSMMIYLNGSDIPEVDWYGNRMVDNDFILIFNAHYEPIMFTL
ECCCCCEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCEEEEC
PDERYGRKWQLVVDTHNPDGPELSYEAGFMITAQSRSFLMLMSDKKPKKPMGA
CCHHHCCEEEEEEECCCCCCCCEEECCCEEEEECCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]