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The map label for this gene is pit [H]

Identifier: 213692506

GI number: 213692506

Start: 1787427

End: 1788479

Strand: Reverse

Name: pit [H]

Synonym: Blon_1635

Alternate gene names: 213692506

Gene position: 1788479-1787427 (Counterclockwise)

Preceding gene: 213692508

Following gene: 213692505

Centisome position: 63.14

GC content: 62.01

Gene sequence:

>1053_bases
TTGGACGTCTCACTGGCGCATTTCCTCCAACAAGTGCTTTCCAACCCGGCTCTGACCGTCACAACGTTGCTGACGCTGGG
AGTCATCTTCGTCAACGGCTGGACCGACGCACCCAACGCCATCGCCACCTGCGTGACCACGCGATGCATGCGCGTACGCT
CCGCCGTCATCATGGCGGCCATCTTCAACTTCCTCGGCGTCTTCATCATGACGCAGCTCAACGCTTCCGTGGCCTCCACC
ATCAGCAACATGGTCGATTTCGGCGGCGACACGAACGCAGCGCTCATCGCCCTATGCGCGGCGCTGTTCTCCATCGTCGT
ATACAGCGTGGGCGCCTCGCTGCTCGGCATACCCACCTCCGAAAGCCACAGCCTGATCGCCGGCCTGTCCGGAGCGGCCA
TCGCCATCCAAGGCGGCATCGGCGGCATCAACATGGGCGAATGGGTCAAGGTGCTCTACGGTCTGGTCGCAAGCCTCCTG
TTCGGCTTCGCCGTCGGCTGGTTGGTATGCAAGGCCGTGACCCTGATTTGCGCTGGCATGGACCGCCGCCGCACCAACGG
GTTCTTCACCTACGCGCAGATCGTGGGCGCGGCGGCCATGAGCTTCATGCATGGCGCGCAGGACGGCCAGAAGTTCATCG
GCGTGCTGTTCCTCGGCATGGCGTTCTGCAACGGCCAACCCAGCGTGACCGGTGTGATGATTCCGGTCTGGCTCATGATT
CTGTGCAGCACCATCATGGGCGTAGGCACGTCCGTCGGCGGTGAACGCATCATCAAATCCGTGGGCCAGGACATGGTGAA
ACTCGAGAAATACCAAGGCTTCTCGGCCGATCTGTCAAGCGCGCTGTGCCTGCTCGTCATGACCGTGCTCGGCATCCCCG
TCTCCACAACGCACACCAAGACCAGCGCCATCATGGGCGTGGGCGCGGTACGCCGCCTCTCCGCCATCAACTTCGGCGTG
GTGCGCGACATGATGCTCACCTGGGTGTTCACCTTCCCCGGTTGCGGTCTCATCAGCTTCGTAATGGCCAAACTGTTCAT
GGTCGTGTTCTAG

Upstream 100 bases:

>100_bases
AACACCATAAAACCCGCGCGCCATGGGAACTACGTAGCCATTCTTCCGTTACAATCAGTGTGACGGCTTCCGCCCCCGAA
GAAGAAGGTGTTGATTCCCC

Downstream 100 bases:

>100_bases
AAAGGAATCCACCCATCATGGCGAGACAAAAAGACGTGTTCTACTTCGACGGATTCCGCCGCAGCGCGGACTACGCATGC
CAGGCCGCGAAGCTGCTGTC

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MDVSLAHFLQQVLSNPALTVTTLLTLGVIFVNGWTDAPNAIATCVTTRCMRVRSAVIMAAIFNFLGVFIMTQLNASVAST
ISNMVDFGGDTNAALIALCAALFSIVVYSVGASLLGIPTSESHSLIAGLSGAAIAIQGGIGGINMGEWVKVLYGLVASLL
FGFAVGWLVCKAVTLICAGMDRRRTNGFFTYAQIVGAAAMSFMHGAQDGQKFIGVLFLGMAFCNGQPSVTGVMIPVWLMI
LCSTIMGVGTSVGGERIIKSVGQDMVKLEKYQGFSADLSSALCLLVMTVLGIPVSTTHTKTSAIMGVGAVRRLSAINFGV
VRDMMLTWVFTFPGCGLISFVMAKLFMVVF

Sequences:

>Translated_350_residues
MDVSLAHFLQQVLSNPALTVTTLLTLGVIFVNGWTDAPNAIATCVTTRCMRVRSAVIMAAIFNFLGVFIMTQLNASVAST
ISNMVDFGGDTNAALIALCAALFSIVVYSVGASLLGIPTSESHSLIAGLSGAAIAIQGGIGGINMGEWVKVLYGLVASLL
FGFAVGWLVCKAVTLICAGMDRRRTNGFFTYAQIVGAAAMSFMHGAQDGQKFIGVLFLGMAFCNGQPSVTGVMIPVWLMI
LCSTIMGVGTSVGGERIIKSVGQDMVKLEKYQGFSADLSSALCLLVMTVLGIPVSTTHTKTSAIMGVGAVRRLSAINFGV
VRDMMLTWVFTFPGCGLISFVMAKLFMVVF
>Mature_350_residues
MDVSLAHFLQQVLSNPALTVTTLLTLGVIFVNGWTDAPNAIATCVTTRCMRVRSAVIMAAIFNFLGVFIMTQLNASVAST
ISNMVDFGGDTNAALIALCAALFSIVVYSVGASLLGIPTSESHSLIAGLSGAAIAIQGGIGGINMGEWVKVLYGLVASLL
FGFAVGWLVCKAVTLICAGMDRRRTNGFFTYAQIVGAAAMSFMHGAQDGQKFIGVLFLGMAFCNGQPSVTGVMIPVWLMI
LCSTIMGVGTSVGGERIIKSVGQDMVKLEKYQGFSADLSSALCLLVMTVLGIPVSTTHTKTSAIMGVGAVRRLSAINFGV
VRDMMLTWVFTFPGCGLISFVMAKLFMVVF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=171, Percent_Identity=32.1637426900585, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI5803173, Length=160, Percent_Identity=31.875, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789907, Length=216, Percent_Identity=31.0185185185185, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789360, Length=215, Percent_Identity=30.2325581395349, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25146401, Length=159, Percent_Identity=27.6729559748428, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539280, Length=163, Percent_Identity=28.8343558282209, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71981576, Length=164, Percent_Identity=28.0487804878049, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=204, Percent_Identity=29.9019607843137, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17557328, Length=162, Percent_Identity=30.2469135802469, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=30.379746835443, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356511, Length=196, Percent_Identity=27.0408163265306, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 36796; Mature: 36796

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVSLAHFLQQVLSNPALTVTTLLTLGVIFVNGWTDAPNAIATCVTTRCMRVRSAVIMAA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IFNFLGVFIMTQLNASVASTISNMVDFGGDTNAALIALCAALFSIVVYSVGASLLGIPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCC
ESHSLIAGLSGAAIAIQGGIGGINMGEWVKVLYGLVASLLFGFAVGWLVCKAVTLICAGM
CCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRRRTNGFFTYAQIVGAAAMSFMHGAQDGQKFIGVLFLGMAFCNGQPSVTGVMIPVWLMI
CCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LCSTIMGVGTSVGGERIIKSVGQDMVKLEKYQGFSADLSSALCLLVMTVLGIPVSTTHTK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
TSAIMGVGAVRRLSAINFGVVRDMMLTWVFTFPGCGLISFVMAKLFMVVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDVSLAHFLQQVLSNPALTVTTLLTLGVIFVNGWTDAPNAIATCVTTRCMRVRSAVIMAA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IFNFLGVFIMTQLNASVASTISNMVDFGGDTNAALIALCAALFSIVVYSVGASLLGIPTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHCCCCCC
ESHSLIAGLSGAAIAIQGGIGGINMGEWVKVLYGLVASLLFGFAVGWLVCKAVTLICAGM
CCCHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DRRRTNGFFTYAQIVGAAAMSFMHGAQDGQKFIGVLFLGMAFCNGQPSVTGVMIPVWLMI
CCHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LCSTIMGVGTSVGGERIIKSVGQDMVKLEKYQGFSADLSSALCLLVMTVLGIPVSTTHTK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
TSAIMGVGAVRRLSAINFGVVRDMMLTWVFTFPGCGLISFVMAKLFMVVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]