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The map label for this gene is metE [H]

Identifier: 213692330

GI number: 213692330

Start: 1616025

End: 1618328

Strand: Reverse

Name: metE [H]

Synonym: Blon_1455

Alternate gene names: 213692330

Gene position: 1618328-1616025 (Counterclockwise)

Preceding gene: 213692331

Following gene: 213692329

Centisome position: 57.13

GC content: 62.28

Gene sequence:

>2304_bases
ATGTCCACTCTGACTTCCGTCTCCGGCTTCCCCCGCATCGGGCAGAACCGTGAACTGAAGAAGATCATCGAAGGTTACTG
GAAGGGTGCCAACGACCTTGCCGCCGTCAAGGCCACCGCCGCCGAACTGCGCGCCAAGCATTGGAGGCTGCAGCAGGCCG
CTGGCATCGATCTCATCGCCAGCAACGACTTCAGCTACTACGATCAGATGCTGGACACCGCCATCCTGCTCAACGTGATT
CCGCAGCGTTACCAGCGTCTCGCCTTCGACGATCAGGAGGATACGCTCTTCGCCATGGCGCGCGGCTACCAGGGCGACAA
GGGTGATGTAACCGCACTGCCGATGAAGAAGTGGTTCACCACCAACTACCACTATCTGGTGCCCGAGGTTGAACCGTCCG
CCGAAATCAAGCTCAATTCCACCAAACCGTTCGATGAATTCAACGAGGCCAAGTCCTTGGGCGTCGAAACCAAGCCCGTG
TTCATCGGACCGTACACCTTCCTCAAGCTCGCGCGTACTCCGGAAGCCGCCGAACTGGAGATCGACAAAGGACTGGTCAA
CGCGGTGGCCGCCGTCTACGCCGAGGTGCTCGCCAGGTTCAACGATCTTGGCGCCGCGTGGGTTCAGCTTGACGAGCCGT
ACCTCGTGCTCGACAAGGAGCCGGGCGATGTCGAGCTCTTCAAGGCCCTCTACACCAAGATCCTGCCCGCCAAGGGCAAT
GTGAAGGTGCTGCTCAACACCTACTTCGGCCACATCGCCGACGTGTATGAGACCGTCAACCTGTTCGGTTTCGACGGCAT
CGGGCTGGATCTCAACGAAGGTCGCGAGGAGAACCTCGAAGCCGTCGCCAAGTACGGCGTGGCCGCCGGCACCACCATCT
TCGCCGGCGTGATTAACGGCCGCAACATCTGGCGCAACAACTACGCCACCTCCCTCGGCTTGGTCGATGCTCTGAAGCAG
GTCACCGCCGATGTGGCCGTCTCCACCGCGTCCTCCTTGCTGCATGTGCCGTTCAGCACCGAAGGGGAGACCGGCATCCC
GGCCGAAGACCTCAAGCACTTCGCATTCGCGGTCCAAAAGCTCGGCGAGCTCAAGGAGATCGCGGCCCTGACCGACGCCA
CCGAGGATGAGAAGAAGTCGTCCGCGGCCCTCGCCGCCAATCAGGCGTTGTTCGACGGCACCCGAGTGGCCGCCGATCCG
GCCGTCGCCGAACGCATCGGCAAGCTGTCCGATGCCGACTACGTGCGTCAGCCGGCCCGCGAAGAGCGCCAGTCCCTGCA
GCGCGAGGCCCTCGGGCTGCCGCTGCTGCCGACCACCACCATCGGCTCCTTCCCCCAGACCAAGGAGATTCGCGCCGAGC
GCGCCAAGCTGCGCAAGGGCGAAGTCACCAAGGAGGCCTATGACGAGTTCATCGCATCGCAGATCGACGCCGTCATCCGC
AAACAGGAGGAGATCGGCCTCGATGTGCTGGTCCATGGCGAATTCGAGCGTAACGACATGGTCGAGTACTTCGGCCAGAA
TCTCAACGGCTTCCTGTTCACCAAGAACGCCTGGGTCCAGTCCTATGGCACTCGTTGCGTCAAGCCCCCGATCGTGTGGG
GCGATGTCTCCCGCGCCAACCCGATCACCGTGGAATGGTCCGCGTACGCCCAGTCCAAGACCGACCATGTGATGAAGGGC
ATGCTCACCGGACCGGTGACCATCCTCAACTGGTCCTGGCCGCGTGAGGACATCACCCATGAGGAACAGACCAAGCAGCT
CGCTCTCGCCATCCGCGACGAAGTCCTCGATCTTGAGGCCGCCGGCATCAAGATCATCCAGATCGATGAGGCCGCCCTGC
GCGAGAAGCTGCCGCTGCGCAAGTCCGACTGGCACGCCAAGTATCTCGACTGGGCCATCCCGGCCTTCCGTCTGGTGCAT
TCCGCCGTCAAGCCGACCACCCAGATCCACACGCACATGTGCTATTCCGAGTTCAACGACATCATCCGCGACATCGATGC
GATGGATGCCGACGTGATCTCCTTCGAGGCGTCCCGCGGCGATCTCGTGGTGCTTGACGCCATCCACGACGCGCGGTTCG
AGACCGAAGCCGGTCCGGGCGTGTACGACATCCATTCCCCGCGCATCCCCTCCGAGAAGGAGATCGAGGATCGCATCTAC
GAGATCCTGGACAAGATCGACGTAAAGAAGGTGTGGATCAACCCCGACTGCGGTCTGAAGACCCGAGGCAACGACGAGAC
CTGGCCGAGCCTTGAACATCTGGTCGCCGCCGCCAAGGCCGTGCGCGCCAGGCTCGACAAGTAG

Upstream 100 bases:

>100_bases
ATTCCGTTGATAGCGACACTCTGCGGCTATTTATAGGGGCCGGAATCCGAATGTTTGCTACCCTGAATCGGGTTACGAAC
GCACACAATTTGGAGGATTC

Downstream 100 bases:

>100_bases
CGGAAATCACCGAGCTCCCTTCCGGATGAGGGAGCTCGGGTTTATGTAAGGAAATCAGCATGCATTCCCCGATGTTCAGC
CTTGAGGTCTTTCCCCCGAA

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 767; Mature: 766

Protein sequence:

>767_residues
MSTLTSVSGFPRIGQNRELKKIIEGYWKGANDLAAVKATAAELRAKHWRLQQAAGIDLIASNDFSYYDQMLDTAILLNVI
PQRYQRLAFDDQEDTLFAMARGYQGDKGDVTALPMKKWFTTNYHYLVPEVEPSAEIKLNSTKPFDEFNEAKSLGVETKPV
FIGPYTFLKLARTPEAAELEIDKGLVNAVAAVYAEVLARFNDLGAAWVQLDEPYLVLDKEPGDVELFKALYTKILPAKGN
VKVLLNTYFGHIADVYETVNLFGFDGIGLDLNEGREENLEAVAKYGVAAGTTIFAGVINGRNIWRNNYATSLGLVDALKQ
VTADVAVSTASSLLHVPFSTEGETGIPAEDLKHFAFAVQKLGELKEIAALTDATEDEKKSSAALAANQALFDGTRVAADP
AVAERIGKLSDADYVRQPAREERQSLQREALGLPLLPTTTIGSFPQTKEIRAERAKLRKGEVTKEAYDEFIASQIDAVIR
KQEEIGLDVLVHGEFERNDMVEYFGQNLNGFLFTKNAWVQSYGTRCVKPPIVWGDVSRANPITVEWSAYAQSKTDHVMKG
MLTGPVTILNWSWPREDITHEEQTKQLALAIRDEVLDLEAAGIKIIQIDEAALREKLPLRKSDWHAKYLDWAIPAFRLVH
SAVKPTTQIHTHMCYSEFNDIIRDIDAMDADVISFEASRGDLVVLDAIHDARFETEAGPGVYDIHSPRIPSEKEIEDRIY
EILDKIDVKKVWINPDCGLKTRGNDETWPSLEHLVAAAKAVRARLDK

Sequences:

>Translated_767_residues
MSTLTSVSGFPRIGQNRELKKIIEGYWKGANDLAAVKATAAELRAKHWRLQQAAGIDLIASNDFSYYDQMLDTAILLNVI
PQRYQRLAFDDQEDTLFAMARGYQGDKGDVTALPMKKWFTTNYHYLVPEVEPSAEIKLNSTKPFDEFNEAKSLGVETKPV
FIGPYTFLKLARTPEAAELEIDKGLVNAVAAVYAEVLARFNDLGAAWVQLDEPYLVLDKEPGDVELFKALYTKILPAKGN
VKVLLNTYFGHIADVYETVNLFGFDGIGLDLNEGREENLEAVAKYGVAAGTTIFAGVINGRNIWRNNYATSLGLVDALKQ
VTADVAVSTASSLLHVPFSTEGETGIPAEDLKHFAFAVQKLGELKEIAALTDATEDEKKSSAALAANQALFDGTRVAADP
AVAERIGKLSDADYVRQPAREERQSLQREALGLPLLPTTTIGSFPQTKEIRAERAKLRKGEVTKEAYDEFIASQIDAVIR
KQEEIGLDVLVHGEFERNDMVEYFGQNLNGFLFTKNAWVQSYGTRCVKPPIVWGDVSRANPITVEWSAYAQSKTDHVMKG
MLTGPVTILNWSWPREDITHEEQTKQLALAIRDEVLDLEAAGIKIIQIDEAALREKLPLRKSDWHAKYLDWAIPAFRLVH
SAVKPTTQIHTHMCYSEFNDIIRDIDAMDADVISFEASRGDLVVLDAIHDARFETEAGPGVYDIHSPRIPSEKEIEDRIY
EILDKIDVKKVWINPDCGLKTRGNDETWPSLEHLVAAAKAVRARLDK
>Mature_766_residues
STLTSVSGFPRIGQNRELKKIIEGYWKGANDLAAVKATAAELRAKHWRLQQAAGIDLIASNDFSYYDQMLDTAILLNVIP
QRYQRLAFDDQEDTLFAMARGYQGDKGDVTALPMKKWFTTNYHYLVPEVEPSAEIKLNSTKPFDEFNEAKSLGVETKPVF
IGPYTFLKLARTPEAAELEIDKGLVNAVAAVYAEVLARFNDLGAAWVQLDEPYLVLDKEPGDVELFKALYTKILPAKGNV
KVLLNTYFGHIADVYETVNLFGFDGIGLDLNEGREENLEAVAKYGVAAGTTIFAGVINGRNIWRNNYATSLGLVDALKQV
TADVAVSTASSLLHVPFSTEGETGIPAEDLKHFAFAVQKLGELKEIAALTDATEDEKKSSAALAANQALFDGTRVAADPA
VAERIGKLSDADYVRQPAREERQSLQREALGLPLLPTTTIGSFPQTKEIRAERAKLRKGEVTKEAYDEFIASQIDAVIRK
QEEIGLDVLVHGEFERNDMVEYFGQNLNGFLFTKNAWVQSYGTRCVKPPIVWGDVSRANPITVEWSAYAQSKTDHVMKGM
LTGPVTILNWSWPREDITHEEQTKQLALAIRDEVLDLEAAGIKIIQIDEAALREKLPLRKSDWHAKYLDWAIPAFRLVHS
AVKPTTQIHTHMCYSEFNDIIRDIDAMDADVISFEASRGDLVVLDAIHDARFETEAGPGVYDIHSPRIPSEKEIEDRIYE
ILDKIDVKKVWINPDCGLKTRGNDETWPSLEHLVAAAKAVRARLDK

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=767, Percent_Identity=46.9361147327249, Blast_Score=654, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=772, Percent_Identity=45.8549222797927, Blast_Score=644, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85445; Mature: 85313

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTSVSGFPRIGQNRELKKIIEGYWKGANDLAAVKATAAELRAKHWRLQQAAGIDLIA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
SNDFSYYDQMLDTAILLNVIPQRYQRLAFDDQEDTLFAMARGYQGDKGDVTALPMKKWFT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEECHHHHHC
TNYHYLVPEVEPSAEIKLNSTKPFDEFNEAKSLGVETKPVFIGPYTFLKLARTPEAAELE
CCCEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCCEEE
IDKGLVNAVAAVYAEVLARFNDLGAAWVQLDEPYLVLDKEPGDVELFKALYTKILPAKGN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VKVLLNTYFGHIADVYETVNLFGFDGIGLDLNEGREENLEAVAKYGVAAGTTIFAGVING
EEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEECCC
RNIWRNNYATSLGLVDALKQVTADVAVSTASSLLHVPFSTEGETGIPAEDLKHFAFAVQK
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
LGELKEIAALTDATEDEKKSSAALAANQALFDGTRVAADPAVAERIGKLSDADYVRQPAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHCCHH
EERQSLQREALGLPLLPTTTIGSFPQTKEIRAERAKLRKGEVTKEAYDEFIASQIDAVIR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KQEEIGLDVLVHGEFERNDMVEYFGQNLNGFLFTKNAWVQSYGTRCVKPPIVWGDVSRAN
HHHHCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCCCEEECCCCCCC
PITVEWSAYAQSKTDHVMKGMLTGPVTILNWSWPREDITHEEQTKQLALAIRDEVLDLEA
CEEEEEHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHH
AGIKIIQIDEAALREKLPLRKSDWHAKYLDWAIPAFRLVHSAVKPTTQIHTHMCYSEFND
CCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IIRDIDAMDADVISFEASRGDLVVLDAIHDARFETEAGPGVYDIHSPRIPSEKEIEDRIY
HHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHH
EILDKIDVKKVWINPDCGLKTRGNDETWPSLEHLVAAAKAVRARLDK
HHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STLTSVSGFPRIGQNRELKKIIEGYWKGANDLAAVKATAAELRAKHWRLQQAAGIDLIA
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
SNDFSYYDQMLDTAILLNVIPQRYQRLAFDDQEDTLFAMARGYQGDKGDVTALPMKKWFT
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCEEEECHHHHHC
TNYHYLVPEVEPSAEIKLNSTKPFDEFNEAKSLGVETKPVFIGPYTFLKLARTPEAAELE
CCCEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHCCCCCCEEE
IDKGLVNAVAAVYAEVLARFNDLGAAWVQLDEPYLVLDKEPGDVELFKALYTKILPAKGN
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHCCCCCC
VKVLLNTYFGHIADVYETVNLFGFDGIGLDLNEGREENLEAVAKYGVAAGTTIFAGVING
EEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHEEEECCC
RNIWRNNYATSLGLVDALKQVTADVAVSTASSLLHVPFSTEGETGIPAEDLKHFAFAVQK
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
LGELKEIAALTDATEDEKKSSAALAANQALFDGTRVAADPAVAERIGKLSDADYVRQPAR
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHCCHH
EERQSLQREALGLPLLPTTTIGSFPQTKEIRAERAKLRKGEVTKEAYDEFIASQIDAVIR
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
KQEEIGLDVLVHGEFERNDMVEYFGQNLNGFLFTKNAWVQSYGTRCVKPPIVWGDVSRAN
HHHHCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCCCEEECCCCCCC
PITVEWSAYAQSKTDHVMKGMLTGPVTILNWSWPREDITHEEQTKQLALAIRDEVLDLEA
CEEEEEHHHHCCHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHH
AGIKIIQIDEAALREKLPLRKSDWHAKYLDWAIPAFRLVHSAVKPTTQIHTHMCYSEFND
CCEEEEEECHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IIRDIDAMDADVISFEASRGDLVVLDAIHDARFETEAGPGVYDIHSPRIPSEKEIEDRIY
HHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHH
EILDKIDVKKVWINPDCGLKTRGNDETWPSLEHLVAAAKAVRARLDK
HHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA