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The map label for this gene is metF [H]

Identifier: 213692329

GI number: 213692329

Start: 1615111

End: 1615965

Strand: Reverse

Name: metF [H]

Synonym: Blon_1454

Alternate gene names: 213692329

Gene position: 1615965-1615111 (Counterclockwise)

Preceding gene: 213692330

Following gene: 213692328

Centisome position: 57.05

GC content: 60.82

Gene sequence:

>855_bases
ATGCATTCCCCGATGTTCAGCCTTGAGGTCTTTCCCCCGAAACGCAACGCTCCCGTGGACACCATCTATGACACCCTTGA
CGGTCTGGAGGGGCTGAACCCCGACTTCATTTCCGTGACCTACGGCCACGGCACCCATTCGGACCGCACCGCCACCGCGC
GCATCGCCCACACGATCAGCAAGGAGTACGGCATTCCCGCAGTCGCGCATCTGACCGCGTTGTACTCCGACAAGGCGAAA
ATCGACGAGGCCCTCGACATGTTCGAGCAGGCCGGTGTGAGCGCGGTGCTCGCCTTGCGAGGCGACTACATCGATGGCGA
ACGTTTGGTCGGCGATTTCAACCATGCCAGCGATCTCGTGTCCTACATCAGATCCATGAAACCGGATCTCACGGTGTTCG
GCGCATGCTATCCAGAAGGCCATTACCAGTCCGGATCGTTGGATCAGGACATCGAGAACCTGAAGATCAAGGTCGATGCC
GGCGTCACCCGTCTGATCTCGCAATTGTTCTATGACAACGAGGATTTCTACCGCTTCCTGGACAAGGCTCGCGCCGCCGG
CATCACCGTGCCGATCGAAGCGGGCATCATGCCGGTGCGCGGCGCCAAGTCGGTGCGGCGCATGGCCGAACGCAACGCCT
CGCGCATTCCCGACAAACTCGATGCACTGCTGAACAGGTGGGAGGATGACATTCAATCGCTGCGTACCGCCGGCATCCTC
TACGCTTCCGAGCAGATCGCCGACCTCGTGGCCCACGGCGTGGACGGCGTCCACCTGTATTCGATGAACCACCCCGCCAC
CACGCGCCGTATCTGGCGCAACGTGGAACCGTTGTTCCTCGGTACGCAAGGTTGA

Upstream 100 bases:

>100_bases
TCGCCGCCGCCAAGGCCGTGCGCGCCAGGCTCGACAAGTAGCGGAAATCACCGAGCTCCCTTCCGGATGAGGGAGCTCGG
GTTTATGTAAGGAAATCAGC

Downstream 100 bases:

>100_bases
CGCATATCGCGGCGCGGCGCGTCTCAATGCGTCGCGCCGCACGTTAACCTGCTGGCGAAACCCAGAGTTCACCCAGCGGC
CGATGGGTATCATGAACGTG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MHSPMFSLEVFPPKRNAPVDTIYDTLDGLEGLNPDFISVTYGHGTHSDRTATARIAHTISKEYGIPAVAHLTALYSDKAK
IDEALDMFEQAGVSAVLALRGDYIDGERLVGDFNHASDLVSYIRSMKPDLTVFGACYPEGHYQSGSLDQDIENLKIKVDA
GVTRLISQLFYDNEDFYRFLDKARAAGITVPIEAGIMPVRGAKSVRRMAERNASRIPDKLDALLNRWEDDIQSLRTAGIL
YASEQIADLVAHGVDGVHLYSMNHPATTRRIWRNVEPLFLGTQG

Sequences:

>Translated_284_residues
MHSPMFSLEVFPPKRNAPVDTIYDTLDGLEGLNPDFISVTYGHGTHSDRTATARIAHTISKEYGIPAVAHLTALYSDKAK
IDEALDMFEQAGVSAVLALRGDYIDGERLVGDFNHASDLVSYIRSMKPDLTVFGACYPEGHYQSGSLDQDIENLKIKVDA
GVTRLISQLFYDNEDFYRFLDKARAAGITVPIEAGIMPVRGAKSVRRMAERNASRIPDKLDALLNRWEDDIQSLRTAGIL
YASEQIADLVAHGVDGVHLYSMNHPATTRRIWRNVEPLFLGTQG
>Mature_284_residues
MHSPMFSLEVFPPKRNAPVDTIYDTLDGLEGLNPDFISVTYGHGTHSDRTATARIAHTISKEYGIPAVAHLTALYSDKAK
IDEALDMFEQAGVSAVLALRGDYIDGERLVGDFNHASDLVSYIRSMKPDLTVFGACYPEGHYQSGSLDQDIENLKIKVDA
GVTRLISQLFYDNEDFYRFLDKARAAGITVPIEAGIMPVRGAKSVRRMAERNASRIPDKLDALLNRWEDDIQSLRTAGIL
YASEQIADLVAHGVDGVHLYSMNHPATTRRIWRNVEPLFLGTQG

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=273, Percent_Identity=32.6007326007326, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1790377, Length=257, Percent_Identity=33.4630350194552, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=30.9352517985612, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=30.9352517985612, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=284, Percent_Identity=33.8028169014084, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321313, Length=269, Percent_Identity=31.9702602230483, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24662658, Length=265, Percent_Identity=25.6603773584906, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31483; Mature: 31483

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSPMFSLEVFPPKRNAPVDTIYDTLDGLEGLNPDFISVTYGHGTHSDRTATARIAHTIS
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
KEYGIPAVAHLTALYSDKAKIDEALDMFEQAGVSAVLALRGDYIDGERLVGDFNHASDLV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHCCCHHHHHHH
SYIRSMKPDLTVFGACYPEGHYQSGSLDQDIENLKIKVDAGVTRLISQLFYDNEDFYRFL
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEECCCHHHHHHHHHCCCHHHHHHH
DKARAAGITVPIEAGIMPVRGAKSVRRMAERNASRIPDKLDALLNRWEDDIQSLRTAGIL
HHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
YASEQIADLVAHGVDGVHLYSMNHPATTRRIWRNVEPLFLGTQG
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEECCC
>Mature Secondary Structure
MHSPMFSLEVFPPKRNAPVDTIYDTLDGLEGLNPDFISVTYGHGTHSDRTATARIAHTIS
CCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHH
KEYGIPAVAHLTALYSDKAKIDEALDMFEQAGVSAVLALRGDYIDGERLVGDFNHASDLV
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCHHHHCCCHHHHHHH
SYIRSMKPDLTVFGACYPEGHYQSGSLDQDIENLKIKVDAGVTRLISQLFYDNEDFYRFL
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEECCCHHHHHHHHHCCCHHHHHHH
DKARAAGITVPIEAGIMPVRGAKSVRRMAERNASRIPDKLDALLNRWEDDIQSLRTAGIL
HHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
YASEQIADLVAHGVDGVHLYSMNHPATTRRIWRNVEPLFLGTQG
HHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]