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The map label for this gene is pyrE [H]

Identifier: 213692320

GI number: 213692320

Start: 1603923

End: 1604618

Strand: Reverse

Name: pyrE [H]

Synonym: Blon_1445

Alternate gene names: 213692320

Gene position: 1604618-1603923 (Counterclockwise)

Preceding gene: 213692321

Following gene: 213692319

Centisome position: 56.65

GC content: 63.22

Gene sequence:

>696_bases
ATGGCAGAAACACTCGCACATCGCTTCACTGAATTCCTGTTGGAATCCAACGCGCTGAAGTTCGGCGACTTCACGCTGAA
GTCCGGCCGCAAGAGCCCGTACTTCATCAACGCCGGCGCCTTCGACGACGGCAAGAAGATCGCCGCGCTCGGCGCGTTCT
ACGCCGAAAAGATTAGCCAGGCCATCGTGCACAACACGATTCCCCGCAACATCGACACCGTGTTCGGCCCCGCCTACAAG
GGCATTCCGCTGGCCGTGTCCACCGCGATCGCGCTGAACGCCGGCCACAACATGACCGTTGGATACACCTTCGACCGCAA
GGAGAAGAAGGACCACGGCGACGGCGGCTGGATGGTCGGCACCCCGCTGACCGACGGCATGAAGGTGCTGCTCGTGGACG
ACGTGATGACCGCCGGCACCGCCGTGCGCGAAGTCATCCCCAAGCTCAAGGCCGAGGCGAACGTCGAGGTCGTGGGTCTC
GTGCTCTCCGTGGACCGCATGGAGAAGACCAAGGACTCCGACCTGTCCGCCGTGAAGGCCGTGGAAGCCGAATTCGGCTT
CCCGGTGCTCTCCATCGCCAACGTGCGCGAGATCTTCGACGCCGCCGCCAAGATGAAGAACTCCGACGGCACCCCATTGC
TCAGCCACGATATCCAGCAGCGCGCCGCCGCCTACCTTGAGGAATACGGCGCCTGA

Upstream 100 bases:

>100_bases
CCCCGCTGCGCGTCGCCCGCGAACTCGACTACCTGCTGGACAGCCGTCCCGAACTCGCTGCTAAACTGGCCGCAGGACAG
ACTTGGAGGTAAGGAACATC

Downstream 100 bases:

>100_bases
TCGTCTTCAACCTAAAACCAAAAGCGGGCTTGCGAGCCCGCTTTTTTTATTACGATCCATGAACAGCAGTACCATATTAA
GGCAGAAGCTTTGGCAAGGA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MAETLAHRFTEFLLESNALKFGDFTLKSGRKSPYFINAGAFDDGKKIAALGAFYAEKISQAIVHNTIPRNIDTVFGPAYK
GIPLAVSTAIALNAGHNMTVGYTFDRKEKKDHGDGGWMVGTPLTDGMKVLLVDDVMTAGTAVREVIPKLKAEANVEVVGL
VLSVDRMEKTKDSDLSAVKAVEAEFGFPVLSIANVREIFDAAAKMKNSDGTPLLSHDIQQRAAAYLEEYGA

Sequences:

>Translated_231_residues
MAETLAHRFTEFLLESNALKFGDFTLKSGRKSPYFINAGAFDDGKKIAALGAFYAEKISQAIVHNTIPRNIDTVFGPAYK
GIPLAVSTAIALNAGHNMTVGYTFDRKEKKDHGDGGWMVGTPLTDGMKVLLVDDVMTAGTAVREVIPKLKAEANVEVVGL
VLSVDRMEKTKDSDLSAVKAVEAEFGFPVLSIANVREIFDAAAKMKNSDGTPLLSHDIQQRAAAYLEEYGA
>Mature_230_residues
AETLAHRFTEFLLESNALKFGDFTLKSGRKSPYFINAGAFDDGKKIAALGAFYAEKISQAIVHNTIPRNIDTVFGPAYKG
IPLAVSTAIALNAGHNMTVGYTFDRKEKKDHGDGGWMVGTPLTDGMKVLLVDDVMTAGTAVREVIPKLKAEANVEVVGLV
LSVDRMEKTKDSDLSAVKAVEAEFGFPVLSIANVREIFDAAAKMKNSDGTPLLSHDIQQRAAAYLEEYGA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790073, Length=192, Percent_Identity=47.3958333333333, Blast_Score=176, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6323530, Length=237, Percent_Identity=38.8185654008439, Blast_Score=154, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6323927, Length=202, Percent_Identity=41.5841584158416, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 24929; Mature: 24798

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETLAHRFTEFLLESNALKFGDFTLKSGRKSPYFINAGAFDDGKKIAALGAFYAEKISQ
CCHHHHHHHHHHHHHCCCCEECCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AIVHNTIPRNIDTVFGPAYKGIPLAVSTAIALNAGHNMTVGYTFDRKEKKDHGDGGWMVG
HHHHHCCCCCCHHHHCCCCCCCCCEEHEEEEEECCCCEEEEEEECCCCCCCCCCCCEEEC
TPLTDGMKVLLVDDVMTAGTAVREVIPKLKAEANVEVVGLVLSVDRMEKTKDSDLSAVKA
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHCCCCHHHHHHH
VEAEFGFPVLSIANVREIFDAAAKMKNSDGTPLLSHDIQQRAAAYLEEYGA
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AETLAHRFTEFLLESNALKFGDFTLKSGRKSPYFINAGAFDDGKKIAALGAFYAEKISQ
CHHHHHHHHHHHHHCCCCEECCEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHH
AIVHNTIPRNIDTVFGPAYKGIPLAVSTAIALNAGHNMTVGYTFDRKEKKDHGDGGWMVG
HHHHHCCCCCCHHHHCCCCCCCCCEEHEEEEEECCCCEEEEEEECCCCCCCCCCCCEEEC
TPLTDGMKVLLVDDVMTAGTAVREVIPKLKAEANVEVVGLVLSVDRMEKTKDSDLSAVKA
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHCCCCHHHHHHH
VEAEFGFPVLSIANVREIFDAAAKMKNSDGTPLLSHDIQQRAAAYLEEYGA
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA