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The map label for this gene is hisF [H]

Identifier: 213691947

GI number: 213691947

Start: 1246068

End: 1246838

Strand: Direct

Name: hisF [H]

Synonym: Blon_1063

Alternate gene names: 213691947

Gene position: 1246068-1246838 (Clockwise)

Preceding gene: 213691945

Following gene: 213691948

Centisome position: 43.99

GC content: 62.39

Gene sequence:

>771_bases
ATGTCGTTGGCGGTACGAGTCATCCCGTGCCTGGACGTTGACGCCGGTCGAGTGGTGAAGGGTGTGCATTTCGAGAACCT
CAAGGACGCGGGTGACCCGGTGGAGCTGGCGGCCGAATACTACCGTCAGGGAGCCGACGAGATCACCTTCCTGGATGTGA
CCGCATCCAGCTCCCACCGCAACACGATGATTGACGTGGTCTCCCGCACCGCCGAACAGGTGTTCATTCCGATGACCGTG
GGCGGCGGCGTACGCACGCCCGAGGACGTCGACTCACTGCTGCGCTGCGGTGCAGACAAGGTCGGTGTCAACACGGCGGC
CATCAACGACCCGTCGCTTATCAGCCGTGTGGCCGATCGTTTCGGCAACCAGGTGCTGGTGCTCTCCGTGGATGCCCGCC
GCGAAAAGGGTGAGCAGCACACGCAGTCGGGTTTTGAGGTCACCACGATGGGCGGCCGCAAATCCACCGGTATTGATGCC
ATCTGGTGGGTCAAGCGCGCCGAGCAGCTTGGCGCCGGCGAGATTCTGCTTAACTCGATGGACGCCGACGGCACCAAGGA
AGGCTTTGACCTCGAGATGATTCGCGCCGTGCGCAAGGAAGTCAAGATTCCGATCATCGCCTCCGGCGGTGCCGGCAAGG
TCGAGGACTTCCCGCCCGCCATCGAAGCAGGTGCCGACGCGGTGCTGGCTGCATCCGTATTCCATTACGGCATCCTGACT
ATCGCCGATGTCAAGGCGGAACTCAAGAAGCACGGCTACACCGTTCGCTAA

Upstream 100 bases:

>100_bases
TCAGCTCGGATTCTCATATTCCAGAAACTGGTGCGGCGCATGTAACGGGCGGTAGGTAGTCTGAAGGACAGTAATGCAAA
GCCGAACAGGGGAGTGAAGC

Downstream 100 bases:

>100_bases
ACGAATACACCTTGCTCCCGCTGGGCGAACAATCCAGTGGATTGTTCGTGGCAGTGCGCTATGCGCACGTCGCACATAAT
CCGGGAGCTGTCAGCGAAGC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSLAVRVIPCLDVDAGRVVKGVHFENLKDAGDPVELAAEYYRQGADEITFLDVTASSSHRNTMIDVVSRTAEQVFIPMTV
GGGVRTPEDVDSLLRCGADKVGVNTAAINDPSLISRVADRFGNQVLVLSVDARREKGEQHTQSGFEVTTMGGRKSTGIDA
IWWVKRAEQLGAGEILLNSMDADGTKEGFDLEMIRAVRKEVKIPIIASGGAGKVEDFPPAIEAGADAVLAASVFHYGILT
IADVKAELKKHGYTVR

Sequences:

>Translated_256_residues
MSLAVRVIPCLDVDAGRVVKGVHFENLKDAGDPVELAAEYYRQGADEITFLDVTASSSHRNTMIDVVSRTAEQVFIPMTV
GGGVRTPEDVDSLLRCGADKVGVNTAAINDPSLISRVADRFGNQVLVLSVDARREKGEQHTQSGFEVTTMGGRKSTGIDA
IWWVKRAEQLGAGEILLNSMDADGTKEGFDLEMIRAVRKEVKIPIIASGGAGKVEDFPPAIEAGADAVLAASVFHYGILT
IADVKAELKKHGYTVR
>Mature_255_residues
SLAVRVIPCLDVDAGRVVKGVHFENLKDAGDPVELAAEYYRQGADEITFLDVTASSSHRNTMIDVVSRTAEQVFIPMTVG
GGVRTPEDVDSLLRCGADKVGVNTAAINDPSLISRVADRFGNQVLVLSVDARREKGEQHTQSGFEVTTMGGRKSTGIDAI
WWVKRAEQLGAGEILLNSMDADGTKEGFDLEMIRAVRKEVKIPIIASGGAGKVEDFPPAIEAGADAVLAASVFHYGILTI
ADVKAELKKHGYTVR

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=45.5598455598456, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=26.9387755102041, Blast_Score=86, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=37.3417721518987, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27453; Mature: 27321

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAVRVIPCLDVDAGRVVKGVHFENLKDAGDPVELAAEYYRQGADEITFLDVTASSSHR
CCCEEEEEEEECCCCCCEEECCCHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
NTMIDVVSRTAEQVFIPMTVGGGVRTPEDVDSLLRCGADKVGVNTAAINDPSLISRVADR
HHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHH
FGNQVLVLSVDARREKGEQHTQSGFEVTTMGGRKSTGIDAIWWVKRAEQLGAGEILLNSM
HCCEEEEEEECCHHHCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECC
DADGTKEGFDLEMIRAVRKEVKIPIIASGGAGKVEDFPPAIEAGADAVLAASVFHYGILT
CCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
IADVKAELKKHGYTVR
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SLAVRVIPCLDVDAGRVVKGVHFENLKDAGDPVELAAEYYRQGADEITFLDVTASSSHR
CCEEEEEEEECCCCCCEEECCCHHCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
NTMIDVVSRTAEQVFIPMTVGGGVRTPEDVDSLLRCGADKVGVNTAAINDPSLISRVADR
HHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEECCCCHHHHHHHHHH
FGNQVLVLSVDARREKGEQHTQSGFEVTTMGGRKSTGIDAIWWVKRAEQLGAGEILLNSM
HCCEEEEEEECCHHHCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECC
DADGTKEGFDLEMIRAVRKEVKIPIIASGGAGKVEDFPPAIEAGADAVLAASVFHYGILT
CCCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
IADVKAELKKHGYTVR
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA