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The map label for this gene is rlmN [H]

Identifier: 213691945

GI number: 213691945

Start: 1244283

End: 1245452

Strand: Direct

Name: rlmN [H]

Synonym: Blon_1061

Alternate gene names: 213691945

Gene position: 1244283-1245452 (Clockwise)

Preceding gene: 213691944

Following gene: 213691947

Centisome position: 43.92

GC content: 62.14

Gene sequence:

>1170_bases
ATGACCACACAGCATCCTGACACTCCGGAAACCGGCATTACTCCCGGCGGCACATCAGGTGCCTTCCGTGACGTGCTATC
CAAGGACCATGCCCGCCGTGGTAAGCCGCCGCTGCACTTTGTGGATATGACGCCCGAGCAGCGCATCGAGAAAGCGGCCG
AACTCGGATTGCCGAAGTTCCGAGTCAAGCAGCTCGCCAACCATTACTTTGGCCATTTCGATGTAAATGCCGCTGAATTC
ACTGATTTTCCGGCAGCCAAGCGCAGCGAGGCCGCCGCCGCGTTCTTCCCGCAGCTCATCACCGAGGTGACCCGCCAAGT
GGCGGACGAAGGCACCACCATCAAGACCTTGTGGAAGCTGTTCGACGGCTCCCTCATCGAATCGGTGCTTATGCGATACC
CGACCCGCACCACGCTGTGCATCTCCTCGCAGGTCGGCTGCGGCATGGGCTGCCCGTTCTGCGCCACCGGCAAGCTTGGC
CTGACCCGTAACATGTCCACCGGTGAAATAATCGAACAGGTGCGTGTGGCCGCCAAAATGATGCGCGACGGCGAAGTGGC
CGGGGGAGCGGGCCGACTGAGCAACATCGTGTTCATGGGCATGGGCGAGCCGATGGGCAACTACAACTCCGTGCTGTCCG
CCGTACGACAGATTTCCGCCATGCCTCCCGAAGGCTTCGGCATTTCCGCGCGCAACATCACCGTATCCACGGTGGGCGTG
GTCCCCGGCATCAAGAAGCTCACTGCCGAAGGCATTCCCGTGCGTCTGGCCGTTTCCCTGCATGCCCCCAGCGACGAACT
GCGTGACGAGCTGGTGCCGATGAACAAGCGTTTCAACACCACGCAGGTGTTGGATGCCGCGCACGATTATTGGCTGGCCT
CCAAGCGCCGCGTCAGTATCGAATACGCGCTGATGCGCGGCATCAACGACCAGGCCGAGCATGCGCAGTTGCTGGCCAAG
AGGCTTAACCATTACGGCGACAACTGGGCCCACGTCAACCCGATTCCGCTCAACCCCATCGAGGGCTCGAAGTGGACCGC
CTCCAAGCCGGAAGACGAACAGCGGTTCCTGGAGATCCTGCACCGCGCGGGCATCACCGCCACACTGCGTGACACCCGCG
GCCAGGATATTGACGGTGCCTGCGGGCAGTTGGCCGCCAAGGAGCGGTAG

Upstream 100 bases:

>100_bases
TGGACCATCTGATTCGTATGCGGCACGCCGTATGGTGGATTCTGATATTTCTATTGCATGTTTGACCATTTTCTGATATT
GAGAGCGAAGCGAACCCATC

Downstream 100 bases:

>100_bases
TTTCGGTGACGGAATCGCCTGCGGCCGATTCCGTCACTTCAGCGCATCCTTGATTGCGCCCACGAAGTAGTCCAGATCGT
CGGGCTTGCGGGACGTGATG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTTQHPDTPETGITPGGTSGAFRDVLSKDHARRGKPPLHFVDMTPEQRIEKAAELGLPKFRVKQLANHYFGHFDVNAAEF
TDFPAAKRSEAAAAFFPQLITEVTRQVADEGTTIKTLWKLFDGSLIESVLMRYPTRTTLCISSQVGCGMGCPFCATGKLG
LTRNMSTGEIIEQVRVAAKMMRDGEVAGGAGRLSNIVFMGMGEPMGNYNSVLSAVRQISAMPPEGFGISARNITVSTVGV
VPGIKKLTAEGIPVRLAVSLHAPSDELRDELVPMNKRFNTTQVLDAAHDYWLASKRRVSIEYALMRGINDQAEHAQLLAK
RLNHYGDNWAHVNPIPLNPIEGSKWTASKPEDEQRFLEILHRAGITATLRDTRGQDIDGACGQLAAKER

Sequences:

>Translated_389_residues
MTTQHPDTPETGITPGGTSGAFRDVLSKDHARRGKPPLHFVDMTPEQRIEKAAELGLPKFRVKQLANHYFGHFDVNAAEF
TDFPAAKRSEAAAAFFPQLITEVTRQVADEGTTIKTLWKLFDGSLIESVLMRYPTRTTLCISSQVGCGMGCPFCATGKLG
LTRNMSTGEIIEQVRVAAKMMRDGEVAGGAGRLSNIVFMGMGEPMGNYNSVLSAVRQISAMPPEGFGISARNITVSTVGV
VPGIKKLTAEGIPVRLAVSLHAPSDELRDELVPMNKRFNTTQVLDAAHDYWLASKRRVSIEYALMRGINDQAEHAQLLAK
RLNHYGDNWAHVNPIPLNPIEGSKWTASKPEDEQRFLEILHRAGITATLRDTRGQDIDGACGQLAAKER
>Mature_388_residues
TTQHPDTPETGITPGGTSGAFRDVLSKDHARRGKPPLHFVDMTPEQRIEKAAELGLPKFRVKQLANHYFGHFDVNAAEFT
DFPAAKRSEAAAAFFPQLITEVTRQVADEGTTIKTLWKLFDGSLIESVLMRYPTRTTLCISSQVGCGMGCPFCATGKLGL
TRNMSTGEIIEQVRVAAKMMRDGEVAGGAGRLSNIVFMGMGEPMGNYNSVLSAVRQISAMPPEGFGISARNITVSTVGVV
PGIKKLTAEGIPVRLAVSLHAPSDELRDELVPMNKRFNTTQVLDAAHDYWLASKRRVSIEYALMRGINDQAEHAQLLAKR
LNHYGDNWAHVNPIPLNPIEGSKWTASKPEDEQRFLEILHRAGITATLRDTRGQDIDGACGQLAAKER

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=355, Percent_Identity=39.7183098591549, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42565; Mature: 42434

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQHPDTPETGITPGGTSGAFRDVLSKDHARRGKPPLHFVDMTPEQRIEKAAELGLPKF
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHH
RVKQLANHYFGHFDVNAAEFTDFPAAKRSEAAAAFFPQLITEVTRQVADEGTTIKTLWKL
HHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FDGSLIESVLMRYPTRTTLCISSQVGCGMGCPFCATGKLGLTRNMSTGEIIEQVRVAAKM
HCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MRDGEVAGGAGRLSNIVFMGMGEPMGNYNSVLSAVRQISAMPPEGFGISARNITVSTVGV
HHCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCEEEEECCC
VPGIKKLTAEGIPVRLAVSLHAPSDELRDELVPMNKRFNTTQVLDAAHDYWLASKRRVSI
CCCHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
EYALMRGINDQAEHAQLLAKRLNHYGDNWAHVNPIPLNPIEGSKWTASKPEDEQRFLEIL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HRAGITATLRDTRGQDIDGACGQLAAKER
HHCCCEEEECCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
TTQHPDTPETGITPGGTSGAFRDVLSKDHARRGKPPLHFVDMTPEQRIEKAAELGLPKF
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHH
RVKQLANHYFGHFDVNAAEFTDFPAAKRSEAAAAFFPQLITEVTRQVADEGTTIKTLWKL
HHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FDGSLIESVLMRYPTRTTLCISSQVGCGMGCPFCATGKLGLTRNMSTGEIIEQVRVAAKM
HCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
MRDGEVAGGAGRLSNIVFMGMGEPMGNYNSVLSAVRQISAMPPEGFGISARNITVSTVGV
HHCCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCEEEEECCC
VPGIKKLTAEGIPVRLAVSLHAPSDELRDELVPMNKRFNTTQVLDAAHDYWLASKRRVSI
CCCHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
EYALMRGINDQAEHAQLLAKRLNHYGDNWAHVNPIPLNPIEGSKWTASKPEDEQRFLEIL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HRAGITATLRDTRGQDIDGACGQLAAKER
HHCCCEEEECCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]