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The map label for this gene is yfiK [H]

Identifier: 213691932

GI number: 213691932

Start: 1229529

End: 1230212

Strand: Direct

Name: yfiK [H]

Synonym: Blon_1048

Alternate gene names: 213691932

Gene position: 1229529-1230212 (Clockwise)

Preceding gene: 213691931

Following gene: 213691933

Centisome position: 43.4

GC content: 50.0

Gene sequence:

>684_bases
ATGATTAACGTAATGATCGTGGACGATCAGCAGCCGACCAGAATGGGTTTTTCGCTCATGGTCATGAAAGATCCTGCGCT
TCGCATGACGTTGCAGGCGGGGAATGGACAGGATGCCCTTGATATTCTTGCGGCACTGCAATTACATGGGCAGAGCCTCC
CTGACGTCATTCTTATGGATGTTCGCATGCCTGTTATGGATGGGCTCGATGCCACGGCAAGAATCAAACAGTTATATCCT
TCAATACGTATCCTGATTTTAACCACATACGATCAGGATAGCTATGCGTTTGGGGGATTGAGCGCCGGAGCTTCGGGATT
CTTGCTTAAGGACGTTAAGACTAAGGACCTGTGTCGGGCCATTCACTCGGTGTACGATGGAGACGCCATACTCACACCAC
GGATTACAAAGGAAGTGATTCGCCGAGGAGTTCCCAACGTCAGCATCAATGCTGACACTCTGGCCATCAGAAGTAAATTC
GATTCATTGGGACCTCGCGAATTCGAAGTCTCAGCACTGGTCAGCGAAGGAATGACTAACGCTGAAATTGCTCAGCGTCT
CGGAATGCAACTGGAGGCAGTCAAAAAGGCGGTTAGTAGGTCTCTTGCCAAGCTCGATGTCAAAGAACGTGTTGGTATCG
CGGTGCTCTGGTATAAGGCCGGAATGCCGAATCTGCAGAAGTGA

Upstream 100 bases:

>100_bases
GGGCACCTTCACTATGGCTCATCATCAGGGCAGGGGTGGACTGTTTCAGCCATTATTCCACAGCAAAAATGTCCATATAT
TCCTGTGCAGGAGGGAAACG

Downstream 100 bases:

>100_bases
CAGATTCCGTAGAATAATGTAGGGTTTCTGAATCGTGAAGGGCTCGTAGCGGGCGAACCCGTGACGGTCCATGACTGTCC
TCGTTTGAATGGCGATCGGC

Product: two component transcriptional regulator, LuxR family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MINVMIVDDQQPTRMGFSLMVMKDPALRMTLQAGNGQDALDILAALQLHGQSLPDVILMDVRMPVMDGLDATARIKQLYP
SIRILILTTYDQDSYAFGGLSAGASGFLLKDVKTKDLCRAIHSVYDGDAILTPRITKEVIRRGVPNVSINADTLAIRSKF
DSLGPREFEVSALVSEGMTNAEIAQRLGMQLEAVKKAVSRSLAKLDVKERVGIAVLWYKAGMPNLQK

Sequences:

>Translated_227_residues
MINVMIVDDQQPTRMGFSLMVMKDPALRMTLQAGNGQDALDILAALQLHGQSLPDVILMDVRMPVMDGLDATARIKQLYP
SIRILILTTYDQDSYAFGGLSAGASGFLLKDVKTKDLCRAIHSVYDGDAILTPRITKEVIRRGVPNVSINADTLAIRSKF
DSLGPREFEVSALVSEGMTNAEIAQRLGMQLEAVKKAVSRSLAKLDVKERVGIAVLWYKAGMPNLQK
>Mature_227_residues
MINVMIVDDQQPTRMGFSLMVMKDPALRMTLQAGNGQDALDILAALQLHGQSLPDVILMDVRMPVMDGLDATARIKQLYP
SIRILILTTYDQDSYAFGGLSAGASGFLLKDVKTKDLCRAIHSVYDGDAILTPRITKEVIRRGVPNVSINADTLAIRSKF
DSLGPREFEVSALVSEGMTNAEIAQRLGMQLEAVKKAVSRSLAKLDVKERVGIAVLWYKAGMPNLQK

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=218, Percent_Identity=30.7339449541284, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788222, Length=226, Percent_Identity=26.1061946902655, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1787473, Length=216, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1790102, Length=214, Percent_Identity=28.9719626168224, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24862; Mature: 24862

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVMIVDDQQPTRMGFSLMVMKDPALRMTLQAGNGQDALDILAALQLHGQSLPDVILMD
CEEEEEECCCCCCHHCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEE
VRMPVMDGLDATARIKQLYPSIRILILTTYDQDSYAFGGLSAGASGFLLKDVKTKDLCRA
CCCHHHCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHH
IHSVYDGDAILTPRITKEVIRRGVPNVSINADTLAIRSKFDSLGPREFEVSALVSEGMTN
HHHHHCCCEEECCHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCCHHHHHHHCCCCH
AEIAQRLGMQLEAVKKAVSRSLAKLDVKERVGIAVLWYKAGMPNLQK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
>Mature Secondary Structure
MINVMIVDDQQPTRMGFSLMVMKDPALRMTLQAGNGQDALDILAALQLHGQSLPDVILMD
CEEEEEECCCCCCHHCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEE
VRMPVMDGLDATARIKQLYPSIRILILTTYDQDSYAFGGLSAGASGFLLKDVKTKDLCRA
CCCHHHCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEECCCHHHHHHH
IHSVYDGDAILTPRITKEVIRRGVPNVSINADTLAIRSKFDSLGPREFEVSALVSEGMTN
HHHHHCCCEEECCHHHHHHHHCCCCCCEECCHHHHHHHHHHCCCCCCCHHHHHHHCCCCH
AEIAQRLGMQLEAVKKAVSRSLAKLDVKERVGIAVLWYKAGMPNLQK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]