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The map label for this gene is spaR [H]

Identifier: 213691891

GI number: 213691891

Start: 1187803

End: 1188468

Strand: Direct

Name: spaR [H]

Synonym: Blon_1003

Alternate gene names: 213691891

Gene position: 1187803-1188468 (Clockwise)

Preceding gene: 213691890

Following gene: 213691892

Centisome position: 41.93

GC content: 45.65

Gene sequence:

>666_bases
ATGGCGACAATCCTGATGATTGATGATGATCGACCCATCTTGGAACTTGTGAAGAACGGACTGGAAAAAGATGGACATTC
AGTGACGATCCATACGTCCGCCGCCCATGTGCCCCTTGAAGGGCTGAACGGGTATGATCTGATTATATTGGACATCATGA
TGCCTGATATGGACGGATTCACCTACTGCGGCAAGATACGTTCGTTGGTGGACTGTCCCATTCTCTTTCTGACCGCTAAG
ACAATGGAGAGAGACATTACATCCGGTCTTGGACTAGGAGCGGATGACTATCTGACAAAACCATTTCGTATTGCGGAATT
ACGTGCGAGAGTACATGCGCATCTACGCCGTGAACAGAGAGAACGACATGTGTCGCTTACGTTTGATCGGATAAAGATTG
ATCTATCCGCAAAAGAACTGCTGGTGGATAATATACCTGTACCTTTGACGAAAAGCGAATATCTGATCTGCGAATATCTT
GCCGGCAACAGAGGGCAAGTTTTTTCAAAGAAACAAATATACGAAGCCGTTTTTCCCTTCTATGGGTATGGCGATGGTTC
TACAATTTCGACGCATATAAAAAACATAAGGTCGAAGTTCGAGGAGGCCGGTGTTCAACCAATCGTAACGGTCTGGGGGA
TAGGCTATAAATGGGAACAAAGATGA

Upstream 100 bases:

>100_bases
GTTGCGGTGCGGATAAATGGCGTCTTCTATGTTGCAAAGCGCCAATGACCGTTTTATTGCATGCGTGAATTATGGAATAG
AAGAGGCAGGCAGGTGTATC

Downstream 100 bases:

>100_bases
AGACGACGCTTACAAGAACTTTTTATAGATTTCTTGTATGTCTGCTTTGCGGACTGTGTATGTCCGTAGTCATCCCCTTT
GCCTGCATGATGTTCGCCTC

Product: two component transcriptional regulator, winged helix family

Products: NA

Alternate protein names: Subtilin biosynthesis regulatory protein spaR [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MATILMIDDDRPILELVKNGLEKDGHSVTIHTSAAHVPLEGLNGYDLIILDIMMPDMDGFTYCGKIRSLVDCPILFLTAK
TMERDITSGLGLGADDYLTKPFRIAELRARVHAHLRREQRERHVSLTFDRIKIDLSAKELLVDNIPVPLTKSEYLICEYL
AGNRGQVFSKKQIYEAVFPFYGYGDGSTISTHIKNIRSKFEEAGVQPIVTVWGIGYKWEQR

Sequences:

>Translated_221_residues
MATILMIDDDRPILELVKNGLEKDGHSVTIHTSAAHVPLEGLNGYDLIILDIMMPDMDGFTYCGKIRSLVDCPILFLTAK
TMERDITSGLGLGADDYLTKPFRIAELRARVHAHLRREQRERHVSLTFDRIKIDLSAKELLVDNIPVPLTKSEYLICEYL
AGNRGQVFSKKQIYEAVFPFYGYGDGSTISTHIKNIRSKFEEAGVQPIVTVWGIGYKWEQR
>Mature_220_residues
ATILMIDDDRPILELVKNGLEKDGHSVTIHTSAAHVPLEGLNGYDLIILDIMMPDMDGFTYCGKIRSLVDCPILFLTAKT
MERDITSGLGLGADDYLTKPFRIAELRARVHAHLRREQRERHVSLTFDRIKIDLSAKELLVDNIPVPLTKSEYLICEYLA
GNRGQVFSKKQIYEAVFPFYGYGDGSTISTHIKNIRSKFEEAGVQPIVTVWGIGYKWEQR

Specific function: Member of the two-component regulatory system spaK/spaR involved in the regulation of the biosynthesis of lantibiotic subtilin. SpaR may function as a regulatory protein [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=34.070796460177, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789809, Length=224, Percent_Identity=31.6964285714286, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI87082012, Length=217, Percent_Identity=30.8755760368664, Blast_Score=109, Evalue=1e-25,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=30.7017543859649, Blast_Score=108, Evalue=2e-25,
Organism=Escherichia coli, GI1790860, Length=220, Percent_Identity=30.9090909090909, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787375, Length=228, Percent_Identity=32.0175438596491, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790552, Length=216, Percent_Identity=30.5555555555556, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789402, Length=216, Percent_Identity=34.2592592592593, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=32.8888888888889, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=29.3333333333333, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=28.5087719298246, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787229, Length=222, Percent_Identity=27.027027027027, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=24.2677824267782, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25043; Mature: 24912

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATILMIDDDRPILELVKNGLEKDGHSVTIHTSAAHVPLEGLNGYDLIILDIMMPDMDGF
CEEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCC
TYCGKIRSLVDCPILFLTAKTMERDITSGLGLGADDYLTKPFRIAELRARVHAHLRREQR
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
ERHVSLTFDRIKIDLSAKELLVDNIPVPLTKSEYLICEYLAGNRGQVFSKKQIYEAVFPF
HCEEEEEEEEEEEECCHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCC
YGYGDGSTISTHIKNIRSKFEEAGVQPIVTVWGIGYKWEQR
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCC
>Mature Secondary Structure 
ATILMIDDDRPILELVKNGLEKDGHSVTIHTSAAHVPLEGLNGYDLIILDIMMPDMDGF
EEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCCC
TYCGKIRSLVDCPILFLTAKTMERDITSGLGLGADDYLTKPFRIAELRARVHAHLRREQR
HHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
ERHVSLTFDRIKIDLSAKELLVDNIPVPLTKSEYLICEYLAGNRGQVFSKKQIYEAVFPF
HCEEEEEEEEEEEECCHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCC
YGYGDGSTISTHIKNIRSKFEEAGVQPIVTVWGIGYKWEQR
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8439156 [H]