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The map label for this gene is dut [H]

Identifier: 213691861

GI number: 213691861

Start: 1152176

End: 1152652

Strand: Direct

Name: dut [H]

Synonym: Blon_0970

Alternate gene names: 213691861

Gene position: 1152176-1152652 (Clockwise)

Preceding gene: 213691860

Following gene: 213691862

Centisome position: 40.67

GC content: 65.83

Gene sequence:

>477_bases
ATGGCGTTCGACGAGACCTACAACGAGCCCGAATCCACCGAGGTCCTGGTCAAAAGCCTCGACCCCGAACACCCGGCCCT
GCTCCGGTACGCGCATGCGGGCGATGCCGGCGCCGATCTCATCACCACCGTCGACGTGACCCTCAAGCCCTTCGAGCGGG
CATTGGTGCCCACCGGCGTGGCCATCGCCCTGCCTGCGGGTTACGTGGCGTTGGTGCACCCGCGTTCCGGGCTGGCGGCC
AAGCAGGGCGTCACCGTGCTCAACGCGCCTGGCACCGTTGATGCAGGCTACCGTGGCGAAATCAAGGTGCCGCTGATCAA
CCTCGATCCGAAGCACACCGCCGTCTTCCATCCGGGCGATCGCATCGCTCAATTGGTGATTCAGCGGTATGTTGAGGCTC
GGTTCATCCCGGCCGAGACACTGCCCGGATCCGATCGCGCCGAACGCGGGTTCGGTTCGACGGGAGTGGCGTCCTGA

Upstream 100 bases:

>100_bases
ACGAGTTCGTCTGCTCGCAGTGCTTCCTGGTCAAGCATCGTAGCCAGCTCGCCTACACGGACGAGGATGGCCAGCCGGTG
TGTGAGGAGTGCGCCGCCTG

Downstream 100 bases:

>100_bases
CCGGATTAGGCCGGTTGAATGACCAAGGTGATGACGGCAACAACGGTTGCGGAACCAATGATGGCGGACGGGTGCGTGCG
CATGGCACTGGTTCGCGGTT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAFDETYNEPESTEVLVKSLDPEHPALLRYAHAGDAGADLITTVDVTLKPFERALVPTGVAIALPAGYVALVHPRSGLAA
KQGVTVLNAPGTVDAGYRGEIKVPLINLDPKHTAVFHPGDRIAQLVIQRYVEARFIPAETLPGSDRAERGFGSTGVAS

Sequences:

>Translated_158_residues
MAFDETYNEPESTEVLVKSLDPEHPALLRYAHAGDAGADLITTVDVTLKPFERALVPTGVAIALPAGYVALVHPRSGLAA
KQGVTVLNAPGTVDAGYRGEIKVPLINLDPKHTAVFHPGDRIAQLVIQRYVEARFIPAETLPGSDRAERGFGSTGVAS
>Mature_157_residues
AFDETYNEPESTEVLVKSLDPEHPALLRYAHAGDAGADLITTVDVTLKPFERALVPTGVAIALPAGYVALVHPRSGLAAK
QGVTVLNAPGTVDAGYRGEIKVPLINLDPKHTAVFHPGDRIAQLVIQRYVEARFIPAETLPGSDRAERGFGSTGVAS

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=133, Percent_Identity=40.6015037593985, Blast_Score=88, Evalue=3e-18,
Organism=Homo sapiens, GI4503423, Length=133, Percent_Identity=40.6015037593985, Blast_Score=87, Evalue=4e-18,
Organism=Homo sapiens, GI70906441, Length=133, Percent_Identity=40.6015037593985, Blast_Score=85, Evalue=3e-17,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=37.5838926174497, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71988561, Length=126, Percent_Identity=44.4444444444444, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319729, Length=121, Percent_Identity=37.1900826446281, Blast_Score=80, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921126, Length=121, Percent_Identity=39.6694214876033, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583610, Length=121, Percent_Identity=39.6694214876033, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16751; Mature: 16620

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDETYNEPESTEVLVKSLDPEHPALLRYAHAGDAGADLITTVDVTLKPFERALVPTGV
CCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEEECCHHHHHCCCCE
AIALPAGYVALVHPRSGLAAKQGVTVLNAPGTVDAGYRGEIKVPLINLDPKHTAVFHPGD
EEEECCCEEEEEECCCCCCHHCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEECCHH
RIAQLVIQRYVEARFIPAETLPGSDRAERGFGSTGVAS
HHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCC
>Mature Secondary Structure 
AFDETYNEPESTEVLVKSLDPEHPALLRYAHAGDAGADLITTVDVTLKPFERALVPTGV
CCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEEECCHHHHHCCCCE
AIALPAGYVALVHPRSGLAAKQGVTVLNAPGTVDAGYRGEIKVPLINLDPKHTAVFHPGD
EEEECCCEEEEEECCCCCCHHCCEEEEECCCCCCCCCCCEEEEEEEECCCCCEEEECCHH
RIAQLVIQRYVEARFIPAETLPGSDRAERGFGSTGVAS
HHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA