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The map label for this gene is dus [H]

Identifier: 213691694

GI number: 213691694

Start: 927929

End: 929173

Strand: Direct

Name: dus [H]

Synonym: Blon_0799

Alternate gene names: 213691694

Gene position: 927929-929173 (Clockwise)

Preceding gene: 213691693

Following gene: 213691695

Centisome position: 32.76

GC content: 61.77

Gene sequence:

>1245_bases
ATGGCACAATCCGAATCCGAAACAGTGCTCACCGAGCATCACGAGACCGACAACCTCGACCCACGTACGGACGCGCCGGC
GCGCCCCGGGACCCCGGTGCATATCGCCCCGGTCCAACTCGGCAACATCACCGTGCCGACCCCGGTGGTGCTCTCGCCGA
TGGCGGGAGTGACCAACTGGCCCTTCCGCGTGATTTGCGAGGAATATGGTCCCGATGGACTCTACGTGGCCGAGATGATC
ACCGCTCGAGCCCTCGTGGCGCATAACCCGAAAGCACTGCGCCTCTGTCATTTCGCCAAAAGCGAAAAAATCCGTTCACT
GCAGTTGTACGGCGTGAACCCCTCCATCACCGAACAGGCGGCGAAAATCGTGGTCGACGGCAACATGGCCGACCATGTGG
ATCTGAACTTCGGTTGCCCGGTGCCCAAGGTCACTCGTCGAGGCGGTGGTTCGGCCTTGCCTTGGAAAACCGACATTTTC
ACTGAGCTATTACAGCGCGTGGTCGCCGTATGCGAGCCGGCGGGCATCCCCGTCACCGCGAAGATCCGCGTCGGCATCGA
CCATAACCATGAGACCTTCCTCGAAGCCGGACACATCGCGCAGGAGGAGGGCTGCAAGGCCGTAACCCTGCATGCCCGTA
CCACCGCCGAATACTATGGCGGTCATTCCGACTGGTCCCGCATCGGCGAGCTCAAGGAACACCTCAACATTCCGGTATTC
GGCAATGGCGATATCTGGGGTGCCGAGGATGCGCTCGAAATGGTGCGCGAGACCGGCTGCGACGGTGTGGCCATCGGCCG
CGGATGCCAGGGCCGGCCGTGGCTGTTCGCCAACATCAAAAACGCCTTCGAGGGCAATCCCGAACGGCTCAACCCGAATC
TGGGCGAGGTCTGCCGCGTGATTCACCGTCATGCCGAGCTGCTTACCGAGTTCTATGAGGGCGATGAGATGATGGCCGTC
CACGATCTGCGCAAGCACATCGCCTGGTATCTGAAAGGATTCCCGGTGGGCGGCTCCACCCGCAAGGCCTTTATGGAATG
CGAGAGCCTGGCCGATGTGGACCGCGAGATCGGCAAGCTCGACCCGACTATCGAATACCCGCCACGCGTGGTCGACAAAC
CGCGCGGCCGCGTGCGTTTCGCCAAGAAGGTGCACTTGCCGTATGGCTGGTTGGAATCGCGTACCACCACCCACGAAGAG
CGTGAGGCGCTCTTCGGTGACGACCCAATGGACGCCAGTTACTGA

Upstream 100 bases:

>100_bases
AAGGAAGACATCCCAGTGGGCTGTTTGTAGGCGAAGTGGCGGCGATAGACGCCGGGGCTCCTCTCTCAGGGGAGCCCCTA
CTTATTTTCTTAGGAATCAC

Downstream 100 bases:

>100_bases
GAAAATACTGAAAATACACGCAACACGCGCCATTCTGCGCGTGAATTTCCCTCCAAGAGTAGCCTGTTTGCGCTACTCTT
GGGTGTAACCGTGAAGTGAC

Product: TIM-barrel protein, nifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MAQSESETVLTEHHETDNLDPRTDAPARPGTPVHIAPVQLGNITVPTPVVLSPMAGVTNWPFRVICEEYGPDGLYVAEMI
TARALVAHNPKALRLCHFAKSEKIRSLQLYGVNPSITEQAAKIVVDGNMADHVDLNFGCPVPKVTRRGGGSALPWKTDIF
TELLQRVVAVCEPAGIPVTAKIRVGIDHNHETFLEAGHIAQEEGCKAVTLHARTTAEYYGGHSDWSRIGELKEHLNIPVF
GNGDIWGAEDALEMVRETGCDGVAIGRGCQGRPWLFANIKNAFEGNPERLNPNLGEVCRVIHRHAELLTEFYEGDEMMAV
HDLRKHIAWYLKGFPVGGSTRKAFMECESLADVDREIGKLDPTIEYPPRVVDKPRGRVRFAKKVHLPYGWLESRTTTHEE
REALFGDDPMDASY

Sequences:

>Translated_414_residues
MAQSESETVLTEHHETDNLDPRTDAPARPGTPVHIAPVQLGNITVPTPVVLSPMAGVTNWPFRVICEEYGPDGLYVAEMI
TARALVAHNPKALRLCHFAKSEKIRSLQLYGVNPSITEQAAKIVVDGNMADHVDLNFGCPVPKVTRRGGGSALPWKTDIF
TELLQRVVAVCEPAGIPVTAKIRVGIDHNHETFLEAGHIAQEEGCKAVTLHARTTAEYYGGHSDWSRIGELKEHLNIPVF
GNGDIWGAEDALEMVRETGCDGVAIGRGCQGRPWLFANIKNAFEGNPERLNPNLGEVCRVIHRHAELLTEFYEGDEMMAV
HDLRKHIAWYLKGFPVGGSTRKAFMECESLADVDREIGKLDPTIEYPPRVVDKPRGRVRFAKKVHLPYGWLESRTTTHEE
REALFGDDPMDASY
>Mature_413_residues
AQSESETVLTEHHETDNLDPRTDAPARPGTPVHIAPVQLGNITVPTPVVLSPMAGVTNWPFRVICEEYGPDGLYVAEMIT
ARALVAHNPKALRLCHFAKSEKIRSLQLYGVNPSITEQAAKIVVDGNMADHVDLNFGCPVPKVTRRGGGSALPWKTDIFT
ELLQRVVAVCEPAGIPVTAKIRVGIDHNHETFLEAGHIAQEEGCKAVTLHARTTAEYYGGHSDWSRIGELKEHLNIPVFG
NGDIWGAEDALEMVRETGCDGVAIGRGCQGRPWLFANIKNAFEGNPERLNPNLGEVCRVIHRHAELLTEFYEGDEMMAVH
DLRKHIAWYLKGFPVGGSTRKAFMECESLADVDREIGKLDPTIEYPPRVVDKPRGRVRFAKKVHLPYGWLESRTTTHEER
EALFGDDPMDASY

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=260, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=32.1888412017167, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI40807366, Length=234, Percent_Identity=29.4871794871795, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI31742496, Length=242, Percent_Identity=28.099173553719, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=330, Percent_Identity=33.6363636363636, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788462, Length=242, Percent_Identity=32.6446280991736, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI145693211, Length=320, Percent_Identity=24.0625, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=236, Percent_Identity=30.9322033898305, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=194, Percent_Identity=29.3814432989691, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=28.755364806867, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=316, Percent_Identity=23.4177215189873, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24585320, Length=282, Percent_Identity=33.6879432624113, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24580595, Length=236, Percent_Identity=29.2372881355932, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19920448, Length=236, Percent_Identity=29.2372881355932, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 45876; Mature: 45744

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSESETVLTEHHETDNLDPRTDAPARPGTPVHIAPVQLGNITVPTPVVLSPMAGVTNW
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCCCHHCCCCCCCCCC
PFRVICEEYGPDGLYVAEMITARALVAHNPKALRLCHFAKSEKIRSLQLYGVNPSITEQA
CCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHCC
AKIVVDGNMADHVDLNFGCPVPKVTRRGGGSALPWKTDIFTELLQRVVAVCEPAGIPVTA
EEEEECCCCCCEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
KIRVGIDHNHETFLEAGHIAQEEGCKAVTLHARTTAEYYGGHSDWSRIGELKEHLNIPVF
EEEEECCCCHHHHHHHCCCHHHCCCCEEEEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEE
GNGDIWGAEDALEMVRETGCDGVAIGRGCQGRPWLFANIKNAFEGNPERLNPNLGEVCRV
CCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECHHHHCCCHHHCCCCHHHHHHH
IHRHAELLTEFYEGDEMMAVHDLRKHIAWYLKGFPVGGSTRKAFMECESLADVDREIGKL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DPTIEYPPRVVDKPRGRVRFAKKVHLPYGWLESRTTTHEEREALFGDDPMDASY
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AQSESETVLTEHHETDNLDPRTDAPARPGTPVHIAPVQLGNITVPTPVVLSPMAGVTNW
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEECCCCHHCCCCCCCCCC
PFRVICEEYGPDGLYVAEMITARALVAHNPKALRLCHFAKSEKIRSLQLYGVNPSITEQA
CCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHCC
AKIVVDGNMADHVDLNFGCPVPKVTRRGGGSALPWKTDIFTELLQRVVAVCEPAGIPVTA
EEEEECCCCCCEEECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEE
KIRVGIDHNHETFLEAGHIAQEEGCKAVTLHARTTAEYYGGHSDWSRIGELKEHLNIPVF
EEEEECCCCHHHHHHHCCCHHHCCCCEEEEEEEEHHHHCCCCHHHHHHHHHHHHCCCCEE
GNGDIWGAEDALEMVRETGCDGVAIGRGCQGRPWLFANIKNAFEGNPERLNPNLGEVCRV
CCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCCEEEEECHHHHCCCHHHCCCCHHHHHHH
IHRHAELLTEFYEGDEMMAVHDLRKHIAWYLKGFPVGGSTRKAFMECESLADVDREIGKL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DPTIEYPPRVVDKPRGRVRFAKKVHLPYGWLESRTTTHEEREALFGDDPMDASY
CCCCCCCCCCCCCCCCCEEEEEEECCCCCHHCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]