Click here to switch to the map view.

The map label for this gene is cscA [H]

Identifier: 213691682

GI number: 213691682

Start: 912483

End: 914039

Strand: Reverse

Name: cscA [H]

Synonym: Blon_0787

Alternate gene names: 213691682

Gene position: 914039-912483 (Counterclockwise)

Preceding gene: 213691683

Following gene: 213691681

Centisome position: 32.27

GC content: 62.17

Gene sequence:

>1557_bases
ATGACTGGCTTCACTCCGGACGCACCCGTCCTCCACGAAATCAAGGACCACAGCGAAGCTCTGGCACAGGCGGAAGCAGG
CGTTGCTGCCATGGCCGCCGAACGCAACAACCGCTGGTACCCGAAGTTCCACATCGCATCCAACGGCGGTTGGATCAACG
ATCCGAACGGCCTGTGCTTCTATAAGGGCCGCTGGCACGTGTTCTACCAGCTGCACCCGTACGGCACCCAGTGGGGTCCG
ATGCACTGGGGACACGTCTCCTCCACCGACATGCTCAATTGGAAACGTGAGCCAATCATGTTCGCCCCGACCCTTGAAGA
GGAGAAGGACGGCGTATTCTCCGGTTCCGCAGTAATCGGCGACGACGGCGAACTCAAGTTCTACTACACCGGCCACCGTT
GGGCCAACGGCCAAGACAACACCGGCGGCGACTGGCAGGTGCAGATGCTCGCCGAGCCGGACAACGACGAGCTGACCAGC
GCCACCAAGCGCGGCATGATCATCGACTGCCCGACCGACAAGGTCGACCATCACTACCGCGACCCGAAGGTCTGGAAGAC
CGGCGACACCTGGTACATGACCTTCGGTGTCTCCTCTGCGGACAAGCGCGGCCAGATGTGGCTGTTCTCCTCCAAGGACA
TGGTCCGCTGGGAATACGAGCGCGTGCTCTTCCAGCACCCGGATCCGGATGTGTTCATGCTCGAATGCCCCGACTTCTTC
CCCATCAAAGACAAGGACGGCAACGAGAAGTGGGTCATTGGCTTCTCCGCCATGGGGTCCAAGCCCAGCGGCTTCATGAA
CCGCAACGTCAACAACGCCGGCTACATGATCGGCACGTGGGAGCCGGGCGGCGAATTCAAGCCGGAAACCGAGTTCCGCC
TGTGGGATTGCGGCCACAACTACTACGCCCCGCAGTCCTTCAATGTGGACGGCCGTCAGATCGTCTATGGCTGGATGAGC
CCGTTCGTGCAGCCGATTCCCATGGAGGATGACGGCTGGTGTGGCCAGCTCACCCTGCCGCGCGAGATCACCCTTGGCGA
TGACGGCGACGTGGTCACCGCGCCGGTCGCAGAAATGGAAGGCCTGCGCGAGGATACCCTCGACCATGGTTCGATCACGC
TCGATATGGATGGGGAGCAGGTCATCGCCGATGACGCCGAGGCCGTGGAAATCGAAATGACCATCGACTTGGCTGCCTCC
ACTGCCGAGCGTGCGGGCCTGAAGATTCATGCCACTGAGGACGGTGCCTACACCTACGTGGCTTATGACGACCAGATCGG
CCGCGTGGTGGTGGATCGTCAGGCCATGGCCAACGGCGATCGCGGCTACCGCGCCGCCCCACTGACCGACACCGAACTGG
CCTCCGGCAAGCTGGACCTGCGCGTGTTCGTGGATCGCGGCTCCGTGGAGGTCTACGTCAACGGTGGCCACCAAGTGCTG
AGCTCCTACTCCTACGCCTCCGAAGGACCGCGTGCCATCAAGCTCGTCGCCGAGTCCGGCTCCCTCAAGGTGGATTCCCT
GAAGCTCCACCACATGAAGTCCATCGGACTGGAGTAG

Upstream 100 bases:

>100_bases
CCGTTTCGGGCAGCATTCTTAAAACTTTTATCTAAAAACTCAATAAGCAACAATTCAACAATCGCAAGATTCATCAATCA
TTAAGAAAGGATATTCAACG

Downstream 100 bases:

>100_bases
CTCTCTCACCTCCCTCTGACGAGGGAGGTTTTTTGTGCGCCCCTGTCGTTCGCCTTTGCCTATACTGATGGACGGCATAT
AACACAATCCATCACATGGC

Product: glycosyl hydrolase family 32, N terminal domain protein

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTGFTPDAPVLHEIKDHSEALAQAEAGVAAMAAERNNRWYPKFHIASNGGWINDPNGLCFYKGRWHVFYQLHPYGTQWGP
MHWGHVSSTDMLNWKREPIMFAPTLEEEKDGVFSGSAVIGDDGELKFYYTGHRWANGQDNTGGDWQVQMLAEPDNDELTS
ATKRGMIIDCPTDKVDHHYRDPKVWKTGDTWYMTFGVSSADKRGQMWLFSSKDMVRWEYERVLFQHPDPDVFMLECPDFF
PIKDKDGNEKWVIGFSAMGSKPSGFMNRNVNNAGYMIGTWEPGGEFKPETEFRLWDCGHNYYAPQSFNVDGRQIVYGWMS
PFVQPIPMEDDGWCGQLTLPREITLGDDGDVVTAPVAEMEGLREDTLDHGSITLDMDGEQVIADDAEAVEIEMTIDLAAS
TAERAGLKIHATEDGAYTYVAYDDQIGRVVVDRQAMANGDRGYRAAPLTDTELASGKLDLRVFVDRGSVEVYVNGGHQVL
SSYSYASEGPRAIKLVAESGSLKVDSLKLHHMKSIGLE

Sequences:

>Translated_518_residues
MTGFTPDAPVLHEIKDHSEALAQAEAGVAAMAAERNNRWYPKFHIASNGGWINDPNGLCFYKGRWHVFYQLHPYGTQWGP
MHWGHVSSTDMLNWKREPIMFAPTLEEEKDGVFSGSAVIGDDGELKFYYTGHRWANGQDNTGGDWQVQMLAEPDNDELTS
ATKRGMIIDCPTDKVDHHYRDPKVWKTGDTWYMTFGVSSADKRGQMWLFSSKDMVRWEYERVLFQHPDPDVFMLECPDFF
PIKDKDGNEKWVIGFSAMGSKPSGFMNRNVNNAGYMIGTWEPGGEFKPETEFRLWDCGHNYYAPQSFNVDGRQIVYGWMS
PFVQPIPMEDDGWCGQLTLPREITLGDDGDVVTAPVAEMEGLREDTLDHGSITLDMDGEQVIADDAEAVEIEMTIDLAAS
TAERAGLKIHATEDGAYTYVAYDDQIGRVVVDRQAMANGDRGYRAAPLTDTELASGKLDLRVFVDRGSVEVYVNGGHQVL
SSYSYASEGPRAIKLVAESGSLKVDSLKLHHMKSIGLE
>Mature_517_residues
TGFTPDAPVLHEIKDHSEALAQAEAGVAAMAAERNNRWYPKFHIASNGGWINDPNGLCFYKGRWHVFYQLHPYGTQWGPM
HWGHVSSTDMLNWKREPIMFAPTLEEEKDGVFSGSAVIGDDGELKFYYTGHRWANGQDNTGGDWQVQMLAEPDNDELTSA
TKRGMIIDCPTDKVDHHYRDPKVWKTGDTWYMTFGVSSADKRGQMWLFSSKDMVRWEYERVLFQHPDPDVFMLECPDFFP
IKDKDGNEKWVIGFSAMGSKPSGFMNRNVNNAGYMIGTWEPGGEFKPETEFRLWDCGHNYYAPQSFNVDGRQIVYGWMSP
FVQPIPMEDDGWCGQLTLPREITLGDDGDVVTAPVAEMEGLREDTLDHGSITLDMDGEQVIADDAEAVEIEMTIDLAAST
AERAGLKIHATEDGAYTYVAYDDQIGRVVVDRQAMANGDRGYRAAPLTDTELASGKLDLRVFVDRGSVEVYVNGGHQVLS
SYSYASEGPRAIKLVAESGSLKVDSLKLHHMKSIGLE

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=364, Percent_Identity=26.6483516483516, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 58078; Mature: 57947

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGFTPDAPVLHEIKDHSEALAQAEAGVAAMAAERNNRWYPKFHIASNGGWINDPNGLCF
CCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHCCCCCCEEEEEECCCCEEECCCCEEE
YKGRWHVFYQLHPYGTQWGPMHWGHVSSTDMLNWKREPIMFAPTLEEEKDGVFSGSAVIG
ECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEECCEEEC
DDGELKFYYTGHRWANGQDNTGGDWQVQMLAEPDNDELTSATKRGMIIDCPTDKVDHHYR
CCCCEEEEECCCEECCCCCCCCCCEEEEEEECCCCCHHHHHHHCCEEEECCCCCCCCCCC
DPKVWKTGDTWYMTFGVSSADKRGQMWLFSSKDMVRWEYERVLFQHPDPDVFMLECPDFF
CCCEEECCCEEEEEEECCCCCCCCCEEEEECCCCEEEEEEEHEEECCCCCEEEEECCCCC
PIKDKDGNEKWVIGFSAMGSKPSGFMNRNVNNAGYMIGTWEPGGEFKPETEFRLWDCGHN
CCCCCCCCCEEEEEEEECCCCCCCHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCC
YYAPQSFNVDGRQIVYGWMSPFVQPIPMEDDGWCGQLTLPREITLGDDGDVVTAPVAEME
CCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCEEEECHHHHC
GLREDTLDHGSITLDMDGEQVIADDAEAVEIEMTIDLAASTAERAGLKIHATEDGAYTYV
CCHHHCCCCCEEEEEECCCEEEECCCCEEEEEEEEEEECCCHHHCCEEEEEECCCEEEEE
AYDDQIGRVVVDRQAMANGDRGYRAAPLTDTELASGKLDLRVFVDRGSVEVYVNGGHQVL
EECCCCCEEEEECHHHCCCCCCEEECCCCCCHHCCCEEEEEEEEECCCEEEEECCCHHHH
SSYSYASEGPRAIKLVAESGSLKVDSLKLHHMKSIGLE
HCCCCCCCCCEEEEEEECCCCEEEEEEEEEHHHHCCCC
>Mature Secondary Structure 
TGFTPDAPVLHEIKDHSEALAQAEAGVAAMAAERNNRWYPKFHIASNGGWINDPNGLCF
CCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEHHCCCCCCEEEEEECCCCEEECCCCEEE
YKGRWHVFYQLHPYGTQWGPMHWGHVSSTDMLNWKREPIMFAPTLEEEKDGVFSGSAVIG
ECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCEEECCEEEC
DDGELKFYYTGHRWANGQDNTGGDWQVQMLAEPDNDELTSATKRGMIIDCPTDKVDHHYR
CCCCEEEEECCCEECCCCCCCCCCEEEEEEECCCCCHHHHHHHCCEEEECCCCCCCCCCC
DPKVWKTGDTWYMTFGVSSADKRGQMWLFSSKDMVRWEYERVLFQHPDPDVFMLECPDFF
CCCEEECCCEEEEEEECCCCCCCCCEEEEECCCCEEEEEEEHEEECCCCCEEEEECCCCC
PIKDKDGNEKWVIGFSAMGSKPSGFMNRNVNNAGYMIGTWEPGGEFKPETEFRLWDCGHN
CCCCCCCCCEEEEEEEECCCCCCCHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCC
YYAPQSFNVDGRQIVYGWMSPFVQPIPMEDDGWCGQLTLPREITLGDDGDVVTAPVAEME
CCCCCCCCCCCCEEEEEEHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCEEEECHHHHC
GLREDTLDHGSITLDMDGEQVIADDAEAVEIEMTIDLAASTAERAGLKIHATEDGAYTYV
CCHHHCCCCCEEEEEECCCEEEECCCCEEEEEEEEEEECCCHHHCCEEEEEECCCEEEEE
AYDDQIGRVVVDRQAMANGDRGYRAAPLTDTELASGKLDLRVFVDRGSVEVYVNGGHQVL
EECCCCCEEEEECHHHCCCCCCEEECCCCCCHHCCCEEEEEEEEECCCEEEEECCCHHHH
SSYSYASEGPRAIKLVAESGSLKVDSLKLHHMKSIGLE
HCCCCCCCCCEEEEEEECCCCEEEEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218016 [H]