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The map label for this gene is braE [H]

Identifier: 213691650

GI number: 213691650

Start: 874742

End: 876556

Strand: Direct

Name: braE [H]

Synonym: Blon_0754

Alternate gene names: 213691650

Gene position: 874742-876556 (Clockwise)

Preceding gene: 213691649

Following gene: 213691651

Centisome position: 30.88

GC content: 61.32

Gene sequence:

>1815_bases
ATGTTTGATGTTCTTTTCGCCGGACTGATACACGGCAGCGCCTATGCGCTGGTCGCAGTCGGCATGTCGCTGATCTTCGG
CGTCACCAACGTGGCCAACTTCGCCCAAGGTTCCATCGTGGCCGTGGGCATGATGATCGCCTGGTGGCTCGGTTCGTCAT
GGGGATGGGGAATCGTGCCCACATTCGTGGCGGTGCTCGTGGTTACCGCACTGCTGGGGCTGATCATGAACGTGGCGATC
ATCGCTCCGCTTGAAGGCCGTAAACCGATTGCGGCGCTGCTCGCCACCATTGGCGTCGGCCAGGTGCTCGACAATGGTCT
GCAGGTCGTCTTCGGGCCACAGACCCGACCGTTCCCGAAACTGCTGCAGACCTATAACCTGCAAGTCGCCGGCGTGCGTT
TCGGCACTTCGGACATCGTGATGGTGACGCTTGCCGTCCTCTCGATGCTTGGCTTATGGGCATTCCTCAAGTTCGGCAAA
GCGGGACAGGCCATTCGCGCCACTTCCCAGGATCCCGAAGCCGCCATGCAGATGGGCATCCCGGTGAAGGCCATCCGTAA
CCTCTCCTTTGTGATCGGTGCCGTACTCGCCGGCGTTTCCGGCATCTTCGTGGGCTTGTTCAACGGCAACATCAATCCGA
TGAATGGCGGCACCATTGGTATGACGGCGTTCGTGGCGGCCACGATCGGCGGTCTAGGCTCCATCCCGGGAGCCGTGGTC
GGCGGTCTTGTGCTCGGTGTGGTGGAAGCGTTCGGCATCTCTCTGTTCGGAGACGGTGCACATGACCTCATCACCTTCGG
CGCGTTGATCATCATCCTCATCGTGAAGCCGCAGGGTCTGCTTGGGCCGAAGACCGATGTCAAATCCGAGCCGCTGACCG
GCACGTTCCTGGGAGGCGGCAGGGCGATCACGCTGCCCTGGTGGGGTAATCTCATCGGTGCCGTGGCACTCGTATCCACC
CCGTTCTTCTTCAATGACTACCTGTCCGGCGTGGGCACTCAGGTCGCCATCTATGCCATCGCCGCCGTGGGACTGACGTT
GATTTCCGGCTCCGCGGGCCAAACGATTCTGGGCATGGCAGGACCGTTCGCCGTGGGTGCCTATGCCTCCGCGATTCTGG
CCACGCAGGCGCATTGGCCCTTCCTGGTGGCGTTGCCCGCCGCAGGTGTGATCGCGGCGCTTGTCGCCTCGGTGCTCACC
TTCCCCGTATGGAGGTTTTCCGGCCATTATGTCGCCATCGCCACCATCGGCGTAGGTGCGGTGATGGTGGCGATCATTCG
CCTCGCCGAGCCGTTGACCAAGGGTTCTCTTGGCATTCAGGGCATTCCGTTCCCGAAGGTGTTCGGCGTCACGCTGTACA
CGCAGCAGATGATGTATCTGCTTGACGTGATCGTGCTGCTTGTCACGCTGGTCATCGTCATGCGTATCCGAACCGCTCAT
CTGGGGCGCGTGTTCGAGGCCGTGGGATCGGATCGCGAGGCTTCGGAGGCGCTGTCCATCCGCACTGGCGCCTATAAGGC
GCTCGCGTATGCGCTTGCCGCGTTTTTCGCAGGTCTCGCCGGTTCGTTGCTCGCGCACCAGTACACGTACATTGATCCGA
CGATCTTCAACTCGAACATGTCGATTCTCATCCTGACCATCATCGTGCTTGGTGGTATGAACTCTCCGCTCGGCGCGGTG
CTTGGCTCCATAGTGCTGATCGGAGCGCCCGAGTTGCTGCGTTTCGCTTCCGACGCGCGCATCCTGCTGTATGGCGTGCT
GCTGGTGCTTGTCATCTTGTTCCGTCCCCAGGGCCTGTTCGCCCGGAAAGGCTGA

Upstream 100 bases:

>100_bases
TCGATCCGATTCTGCTCATCGTCAAAGATGGCAAGTTCGAACAATACGAAGGTTGATTCTCCGGGCTTTCCCATACACCA
CGAACGAGGTAACGCACACA

Downstream 100 bases:

>100_bases
GTCGCCATGACCGATTCCGAGACCGTATTTTCCACCGTAGACGCCGTGACCGGCATCGATATCGACAACGAACGCCTCGA
TAACGCGGATGATCTGGCTG

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MFDVLFAGLIHGSAYALVAVGMSLIFGVTNVANFAQGSIVAVGMMIAWWLGSSWGWGIVPTFVAVLVVTALLGLIMNVAI
IAPLEGRKPIAALLATIGVGQVLDNGLQVVFGPQTRPFPKLLQTYNLQVAGVRFGTSDIVMVTLAVLSMLGLWAFLKFGK
AGQAIRATSQDPEAAMQMGIPVKAIRNLSFVIGAVLAGVSGIFVGLFNGNINPMNGGTIGMTAFVAATIGGLGSIPGAVV
GGLVLGVVEAFGISLFGDGAHDLITFGALIIILIVKPQGLLGPKTDVKSEPLTGTFLGGGRAITLPWWGNLIGAVALVST
PFFFNDYLSGVGTQVAIYAIAAVGLTLISGSAGQTILGMAGPFAVGAYASAILATQAHWPFLVALPAAGVIAALVASVLT
FPVWRFSGHYVAIATIGVGAVMVAIIRLAEPLTKGSLGIQGIPFPKVFGVTLYTQQMMYLLDVIVLLVTLVIVMRIRTAH
LGRVFEAVGSDREASEALSIRTGAYKALAYALAAFFAGLAGSLLAHQYTYIDPTIFNSNMSILILTIIVLGGMNSPLGAV
LGSIVLIGAPELLRFASDARILLYGVLLVLVILFRPQGLFARKG

Sequences:

>Translated_604_residues
MFDVLFAGLIHGSAYALVAVGMSLIFGVTNVANFAQGSIVAVGMMIAWWLGSSWGWGIVPTFVAVLVVTALLGLIMNVAI
IAPLEGRKPIAALLATIGVGQVLDNGLQVVFGPQTRPFPKLLQTYNLQVAGVRFGTSDIVMVTLAVLSMLGLWAFLKFGK
AGQAIRATSQDPEAAMQMGIPVKAIRNLSFVIGAVLAGVSGIFVGLFNGNINPMNGGTIGMTAFVAATIGGLGSIPGAVV
GGLVLGVVEAFGISLFGDGAHDLITFGALIIILIVKPQGLLGPKTDVKSEPLTGTFLGGGRAITLPWWGNLIGAVALVST
PFFFNDYLSGVGTQVAIYAIAAVGLTLISGSAGQTILGMAGPFAVGAYASAILATQAHWPFLVALPAAGVIAALVASVLT
FPVWRFSGHYVAIATIGVGAVMVAIIRLAEPLTKGSLGIQGIPFPKVFGVTLYTQQMMYLLDVIVLLVTLVIVMRIRTAH
LGRVFEAVGSDREASEALSIRTGAYKALAYALAAFFAGLAGSLLAHQYTYIDPTIFNSNMSILILTIIVLGGMNSPLGAV
LGSIVLIGAPELLRFASDARILLYGVLLVLVILFRPQGLFARKG
>Mature_604_residues
MFDVLFAGLIHGSAYALVAVGMSLIFGVTNVANFAQGSIVAVGMMIAWWLGSSWGWGIVPTFVAVLVVTALLGLIMNVAI
IAPLEGRKPIAALLATIGVGQVLDNGLQVVFGPQTRPFPKLLQTYNLQVAGVRFGTSDIVMVTLAVLSMLGLWAFLKFGK
AGQAIRATSQDPEAAMQMGIPVKAIRNLSFVIGAVLAGVSGIFVGLFNGNINPMNGGTIGMTAFVAATIGGLGSIPGAVV
GGLVLGVVEAFGISLFGDGAHDLITFGALIIILIVKPQGLLGPKTDVKSEPLTGTFLGGGRAITLPWWGNLIGAVALVST
PFFFNDYLSGVGTQVAIYAIAAVGLTLISGSAGQTILGMAGPFAVGAYASAILATQAHWPFLVALPAAGVIAALVASVLT
FPVWRFSGHYVAIATIGVGAVMVAIIRLAEPLTKGSLGIQGIPFPKVFGVTLYTQQMMYLLDVIVLLVTLVIVMRIRTAH
LGRVFEAVGSDREASEALSIRTGAYKALAYALAAFFAGLAGSLLAHQYTYIDPTIFNSNMSILILTIIVLGGMNSPLGAV
LGSIVLIGAPELLRFASDARILLYGVLLVLVILFRPQGLFARKG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=298, Percent_Identity=32.8859060402685, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1789865, Length=302, Percent_Identity=31.4569536423841, Blast_Score=133, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 62593; Mature: 62593

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVLFAGLIHGSAYALVAVGMSLIFGVTNVANFAQGSIVAVGMMIAWWLGSSWGWGIVP
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHH
TFVAVLVVTALLGLIMNVAIIAPLEGRKPIAALLATIGVGQVLDNGLQVVFGPQTRPFPK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
LLQTYNLQVAGVRFGTSDIVMVTLAVLSMLGLWAFLKFGKAGQAIRATSQDPEAAMQMGI
HHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHCC
PVKAIRNLSFVIGAVLAGVSGIFVGLFNGNINPMNGGTIGMTAFVAATIGGLGSIPGAVV
CHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
GGLVLGVVEAFGISLFGDGAHDLITFGALIIILIVKPQGLLGPKTDVKSEPLTGTFLGGG
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEEECCC
RAITLPWWGNLIGAVALVSTPFFFNDYLSGVGTQVAIYAIAAVGLTLISGSAGQTILGMA
EEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECC
GPFAVGAYASAILATQAHWPFLVALPAAGVIAALVASVLTFPVWRFSGHYVAIATIGVGA
CCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
VMVAIIRLAEPLTKGSLGIQGIPFPKVFGVTLYTQQMMYLLDVIVLLVTLVIVMRIRTAH
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRVFEAVGSDREASEALSIRTGAYKALAYALAAFFAGLAGSLLAHQYTYIDPTIFNSNM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCH
SILILTIIVLGGMNSPLGAVLGSIVLIGAPELLRFASDARILLYGVLLVLVILFRPQGLF
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
ARKG
CCCC
>Mature Secondary Structure
MFDVLFAGLIHGSAYALVAVGMSLIFGVTNVANFAQGSIVAVGMMIAWWLGSSWGWGIVP
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHH
TFVAVLVVTALLGLIMNVAIIAPLEGRKPIAALLATIGVGQVLDNGLQVVFGPQTRPFPK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHH
LLQTYNLQVAGVRFGTSDIVMVTLAVLSMLGLWAFLKFGKAGQAIRATSQDPEAAMQMGI
HHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHCC
PVKAIRNLSFVIGAVLAGVSGIFVGLFNGNINPMNGGTIGMTAFVAATIGGLGSIPGAVV
CHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
GGLVLGVVEAFGISLFGDGAHDLITFGALIIILIVKPQGLLGPKTDVKSEPLTGTFLGGG
HHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCEEEECCC
RAITLPWWGNLIGAVALVSTPFFFNDYLSGVGTQVAIYAIAAVGLTLISGSAGQTILGMA
EEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEECC
GPFAVGAYASAILATQAHWPFLVALPAAGVIAALVASVLTFPVWRFSGHYVAIATIGVGA
CCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
VMVAIIRLAEPLTKGSLGIQGIPFPKVFGVTLYTQQMMYLLDVIVLLVTLVIVMRIRTAH
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGRVFEAVGSDREASEALSIRTGAYKALAYALAAFFAGLAGSLLAHQYTYIDPTIFNSNM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCH
SILILTIIVLGGMNSPLGAVLGSIVLIGAPELLRFASDARILLYGVLLVLVILFRPQGLF
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
ARKG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]