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The map label for this gene is livJ [C]

Identifier: 213691649

GI number: 213691649

Start: 873534

End: 874697

Strand: Direct

Name: livJ [C]

Synonym: Blon_0753

Alternate gene names: 213691649

Gene position: 873534-874697 (Clockwise)

Preceding gene: 213691648

Following gene: 213691650

Centisome position: 30.84

GC content: 58.08

Gene sequence:

>1164_bases
ATGACCATCAAGACAATCATCCGTACGGCGATCGCATCCGTGGTCACTGCGGGAACACTGATTGGCGTCTCCGCATGCGG
CCTTGGCGGGGATGCCGCCAGCGGTTCCAGCGACGACACCGTGAACATCGGAGTCATCTATCCGAAGACCGGGCAATATG
CGCAATACGGCAAACTGTTCCAGCAAGGCTTCGATCTGGCGGTTGCCAAGGTGAACAAGGATGGCGGCGTCAAGGGTAAG
AAGCTTGGTCTTCGATACTATGACACGCAGTCCGATGCCAAGCAAGACGCCGCGGTGGCTCCCAAGATCGTGTCGGACAA
GTCGATCATCGCCGTGGTGGGTGACTATGCCTCGCCCGCATCCTCGGCCGCGTCGCCCATCTTCCAGCAGGCCGGCCTCG
TGCATTACGGTTTCAATAATTCGGCACCCACCTTTACCGATACCGGGGACCACGTATGGACTCCCCAGATCGGTCAGGAC
AAGTATCAGAAGACCAACGCGGATATAGTGGCCAAAAAAGCCAAGAAGATCGCGGTGGTCTACATCGAGAACGACTGGGG
CAAACAGGCCTATCGGTACTTCAAGCAGGAGGCCGAGAAAAACGGTACCCAGATTGTGTACGAGTCCAGCTATCTGCCCG
ACTCCACCGACTTGTCGCCGATTCTGATTCCGGCGCGCGATGCCAAGCCTGACGCGGTGGTCGACATCGGCTACGGGCCG
GACGGCGCTCTGGTGATCAATACCCTGCGCGACAAGCTGGGGTACGACGGCCTGTACTTCGGTGGTCAGGAAACCAAGGA
GTTCCTCGATCTCGCCGGCAAAAACGCCGAGGGTTCGATCATCACCGGATCCTTCTCCGCCACCGGCACCAAGGACGCCA
AGGCCCAGCAGTTCGTCAAGGACTTCAAGGCCAAGTACGGCAACAACCCGGGCAATTTCGAAGTGACCGCCTACGAGGCG
ATCATCGATCTGGCCTATGCCGCCAACAAGACCGAACCGACCCGTGAAGGCATACAGAAGGCGCTGCAGACCGTGACGGA
TTTCCCGCTCTACCAGGGCAATGGCGGCACGTTCAAATTCGATCGGCAGAGTCGTCGCGCCGATAACATCGATCCGATTC
TGCTCATCGTCAAAGATGGCAAGTTCGAACAATACGAAGGTTGA

Upstream 100 bases:

>100_bases
CGGCGGGCGACGAGGCGCTGCAGGCATTGAGCGACATCCGCGTGGGCTGAGCCGGTCCCCATTTACGCTTCATGATTATT
CGAATAGAGAAGGGAACATC

Downstream 100 bases:

>100_bases
TTCTCCGGGCTTTCCCATACACCACGAACGAGGTAACGCACACAATGTTTGATGTTCTTTTCGCCGGACTGATACACGGC
AGCGCCTATGCGCTGGTCGC

Product: Extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTIKTIIRTAIASVVTAGTLIGVSACGLGGDAASGSSDDTVNIGVIYPKTGQYAQYGKLFQQGFDLAVAKVNKDGGVKGK
KLGLRYYDTQSDAKQDAAVAPKIVSDKSIIAVVGDYASPASSAASPIFQQAGLVHYGFNNSAPTFTDTGDHVWTPQIGQD
KYQKTNADIVAKKAKKIAVVYIENDWGKQAYRYFKQEAEKNGTQIVYESSYLPDSTDLSPILIPARDAKPDAVVDIGYGP
DGALVINTLRDKLGYDGLYFGGQETKEFLDLAGKNAEGSIITGSFSATGTKDAKAQQFVKDFKAKYGNNPGNFEVTAYEA
IIDLAYAANKTEPTREGIQKALQTVTDFPLYQGNGGTFKFDRQSRRADNIDPILLIVKDGKFEQYEG

Sequences:

>Translated_387_residues
MTIKTIIRTAIASVVTAGTLIGVSACGLGGDAASGSSDDTVNIGVIYPKTGQYAQYGKLFQQGFDLAVAKVNKDGGVKGK
KLGLRYYDTQSDAKQDAAVAPKIVSDKSIIAVVGDYASPASSAASPIFQQAGLVHYGFNNSAPTFTDTGDHVWTPQIGQD
KYQKTNADIVAKKAKKIAVVYIENDWGKQAYRYFKQEAEKNGTQIVYESSYLPDSTDLSPILIPARDAKPDAVVDIGYGP
DGALVINTLRDKLGYDGLYFGGQETKEFLDLAGKNAEGSIITGSFSATGTKDAKAQQFVKDFKAKYGNNPGNFEVTAYEA
IIDLAYAANKTEPTREGIQKALQTVTDFPLYQGNGGTFKFDRQSRRADNIDPILLIVKDGKFEQYEG
>Mature_386_residues
TIKTIIRTAIASVVTAGTLIGVSACGLGGDAASGSSDDTVNIGVIYPKTGQYAQYGKLFQQGFDLAVAKVNKDGGVKGKK
LGLRYYDTQSDAKQDAAVAPKIVSDKSIIAVVGDYASPASSAASPIFQQAGLVHYGFNNSAPTFTDTGDHVWTPQIGQDK
YQKTNADIVAKKAKKIAVVYIENDWGKQAYRYFKQEAEKNGTQIVYESSYLPDSTDLSPILIPARDAKPDAVVDIGYGPD
GALVINTLRDKLGYDGLYFGGQETKEFLDLAGKNAEGSIITGSFSATGTKDAKAQQFVKDFKAKYGNNPGNFEVTAYEAI
IDLAYAANKTEPTREGIQKALQTVTDFPLYQGNGGTFKFDRQSRRADNIDPILLIVKDGKFEQYEG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=303, Percent_Identity=27.7227722772277, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789867, Length=298, Percent_Identity=25.8389261744966, Blast_Score=78, Evalue=9e-16,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41612; Mature: 41481

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKTIIRTAIASVVTAGTLIGVSACGLGGDAASGSSDDTVNIGVIYPKTGQYAQYGKLF
CCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHH
QQGFDLAVAKVNKDGGVKGKKLGLRYYDTQSDAKQDAAVAPKIVSDKSIIAVVGDYASPA
HHCCCEEEEEECCCCCCCCCEECEEEECCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCC
SSAASPIFQQAGLVHYGFNNSAPTFTDTGDHVWTPQIGQDKYQKTNADIVAKKAKKIAVV
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHCCHHHHEECCCEEEEE
YIENDWGKQAYRYFKQEAEKNGTQIVYESSYLPDSTDLSPILIPARDAKPDAVVDIGYGP
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEECCCCC
DGALVINTLRDKLGYDGLYFGGQETKEFLDLAGKNAEGSIITGSFSATGTKDAKAQQFVK
CCCEEEEHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHH
DFKAKYGNNPGNFEVTAYEAIIDLAYAANKTEPTREGIQKALQTVTDFPLYQGNGGTFKF
HHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCEEEE
DRQSRRADNIDPILLIVKDGKFEQYEG
CCHHCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TIKTIIRTAIASVVTAGTLIGVSACGLGGDAASGSSDDTVNIGVIYPKTGQYAQYGKLF
CHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHH
QQGFDLAVAKVNKDGGVKGKKLGLRYYDTQSDAKQDAAVAPKIVSDKSIIAVVGDYASPA
HHCCCEEEEEECCCCCCCCCEECEEEECCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCC
SSAASPIFQQAGLVHYGFNNSAPTFTDTGDHVWTPQIGQDKYQKTNADIVAKKAKKIAVV
HHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEECCCCCCHHHHHCCHHHHEECCCEEEEE
YIENDWGKQAYRYFKQEAEKNGTQIVYESSYLPDSTDLSPILIPARDAKPDAVVDIGYGP
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCEEEECCCCC
DGALVINTLRDKLGYDGLYFGGQETKEFLDLAGKNAEGSIITGSFSATGTKDAKAQQFVK
CCCEEEEHHHHHHCCCCEEECCHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHH
DFKAKYGNNPGNFEVTAYEAIIDLAYAANKTEPTREGIQKALQTVTDFPLYQGNGGTFKF
HHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEECCCCEEEE
DRQSRRADNIDPILLIVKDGKFEQYEG
CCHHCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]