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The map label for this gene is yiaE [C]

Identifier: 213691614

GI number: 213691614

Start: 828985

End: 829971

Strand: Direct

Name: yiaE [C]

Synonym: Blon_0718

Alternate gene names: 213691614

Gene position: 828985-829971 (Clockwise)

Preceding gene: 213691612

Following gene: 213691616

Centisome position: 29.26

GC content: 60.79

Gene sequence:

>987_bases
ATGGGTATTGAGAACACGCATAAGCTGATTGTCAACTGCCTGCCTCTCAACGAAGGAGAACGGCGGGCTTTCGCCAAGGC
GGCCCGCGATGTGCCGCAGGAATTCGTGGGCGACGAAGCGCACATCGGCGACATGGTCTGGTCGGCCGCTGTTCCCGAGG
AGCTTCGCTCCCGAGCTACCGCAGTCATCGGCAACTTCCCGGTCTCGCAGGCTTCCCAATACACCCGTCTTGAATGGTTG
CAGACCTTCAGCGCCGGTGTGGATGCGTACATCCGCCCGGGTGTGCTGCCTCGCGGCACCATGGTCACCAATGCGAGCGG
CGCGTATGGCCAAAGCGTGTCGGAACACATGTTCGCCACCATGTGGGCATTGATGAAGAACCTCAACCGGTATGCCTCCA
ATCAGCGTGATCATCAGTGGCGGGATGAAGGCCCGGTATTGAGCCCGGAAGGTGGCATCGCGCTGATTATCGGCGTCGGC
GACATCGGGTCGCATTTCGCTCAGCTTGCCCAATCCGTAGGTATGCGTACCTTCGGCGTGCGCCGTCATGCGGATGTGCC
GGCCAAGGGCATTGAAAAGATGTATGGCTTCGAGCGGCTGGAAGCACTGCTGCCATTGGCGGACGTGGTGGCGATGGCGG
TGCCGCGCTCGCCGCAGACCCATCATCTGCTGAATGCGGATCGTCTGGGTCGTCTGAAATCCACGGCCATCGTGATCAAT
GCCGGGCGTGGCGACGCCATCGATCACGAGGCCTTGGCCGACGCCTTGCACAATGGACGGCTCCACGGTGCCGGGTTGGA
CGTCACCGAGCCTGAGCCGTTGCCGGCGGAAAGTCCGTTGTGGGATGAGCCGCATTGCCTGATCACCCCGCATGTGGCCG
GCGGCAATCATTTGGAAAAGACTTCCGAACACATCATCCGCATCGCCCTCACCAACGTTTCCCGCTATGCCAAACAGCAG
GAACTGTTGAATCTCGTGCGATACTGA

Upstream 100 bases:

>100_bases
TGGGGCGGGTTCGGCATTGGTCCGGCGGGGCGGGTTCGGCATTGGTTCAGCATGAGGAAACACGCGATAATATCCGCCCT
GCCGCCTAGTTTGGTGAGCC

Downstream 100 bases:

>100_bases
TTCGCCTGTATCAGGTGTACATGAACTCATGTACACGGCCGGCGTGTCGCATCCCACGGCGGCTGCGGCATCGAGATAAT
ACCTGCGGAGGCGTGGTTGA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MGIENTHKLIVNCLPLNEGERRAFAKAARDVPQEFVGDEAHIGDMVWSAAVPEELRSRATAVIGNFPVSQASQYTRLEWL
QTFSAGVDAYIRPGVLPRGTMVTNASGAYGQSVSEHMFATMWALMKNLNRYASNQRDHQWRDEGPVLSPEGGIALIIGVG
DIGSHFAQLAQSVGMRTFGVRRHADVPAKGIEKMYGFERLEALLPLADVVAMAVPRSPQTHHLLNADRLGRLKSTAIVIN
AGRGDAIDHEALADALHNGRLHGAGLDVTEPEPLPAESPLWDEPHCLITPHVAGGNHLEKTSEHIIRIALTNVSRYAKQQ
ELLNLVRY

Sequences:

>Translated_328_residues
MGIENTHKLIVNCLPLNEGERRAFAKAARDVPQEFVGDEAHIGDMVWSAAVPEELRSRATAVIGNFPVSQASQYTRLEWL
QTFSAGVDAYIRPGVLPRGTMVTNASGAYGQSVSEHMFATMWALMKNLNRYASNQRDHQWRDEGPVLSPEGGIALIIGVG
DIGSHFAQLAQSVGMRTFGVRRHADVPAKGIEKMYGFERLEALLPLADVVAMAVPRSPQTHHLLNADRLGRLKSTAIVIN
AGRGDAIDHEALADALHNGRLHGAGLDVTEPEPLPAESPLWDEPHCLITPHVAGGNHLEKTSEHIIRIALTNVSRYAKQQ
ELLNLVRY
>Mature_327_residues
GIENTHKLIVNCLPLNEGERRAFAKAARDVPQEFVGDEAHIGDMVWSAAVPEELRSRATAVIGNFPVSQASQYTRLEWLQ
TFSAGVDAYIRPGVLPRGTMVTNASGAYGQSVSEHMFATMWALMKNLNRYASNQRDHQWRDEGPVLSPEGGIALIIGVGD
IGSHFAQLAQSVGMRTFGVRRHADVPAKGIEKMYGFERLEALLPLADVVAMAVPRSPQTHHLLNADRLGRLKSTAIVINA
GRGDAIDHEALADALHNGRLHGAGLDVTEPEPLPAESPLWDEPHCLITPHVAGGNHLEKTSEHIIRIALTNVSRYAKQQE
LLNLVRY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=234, Percent_Identity=26.9230769230769, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI23308577, Length=220, Percent_Identity=27.7272727272727, Blast_Score=88, Evalue=9e-18,
Organism=Homo sapiens, GI145580578, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI4557499, Length=164, Percent_Identity=35.3658536585366, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI61743967, Length=142, Percent_Identity=35.9154929577465, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4557497, Length=142, Percent_Identity=35.9154929577465, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI145580575, Length=164, Percent_Identity=35.3658536585366, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI87082289, Length=232, Percent_Identity=30.1724137931034, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI87081824, Length=200, Percent_Identity=31, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=27.1317829457364, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787645, Length=163, Percent_Identity=28.8343558282209, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=228, Percent_Identity=28.5087719298246, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324055, Length=236, Percent_Identity=28.8135593220339, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320925, Length=257, Percent_Identity=28.4046692607004, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=173, Percent_Identity=32.9479768786127, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322116, Length=261, Percent_Identity=27.9693486590038, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324964, Length=220, Percent_Identity=25, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921140, Length=233, Percent_Identity=30.0429184549356, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585516, Length=245, Percent_Identity=27.7551020408163, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574286, Length=135, Percent_Identity=32.5925925925926, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571528, Length=114, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24646446, Length=142, Percent_Identity=35.2112676056338, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646448, Length=142, Percent_Identity=35.2112676056338, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646452, Length=142, Percent_Identity=35.2112676056338, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646450, Length=142, Percent_Identity=35.2112676056338, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472511, Length=142, Percent_Identity=35.2112676056338, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45552429, Length=162, Percent_Identity=30.2469135802469, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574284, Length=162, Percent_Identity=30.2469135802469, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45551003, Length=162, Percent_Identity=30.2469135802469, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585514, Length=248, Percent_Identity=25, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28574282, Length=248, Percent_Identity=25, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: NA

Molecular weight: Translated: 35899; Mature: 35768

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIENTHKLIVNCLPLNEGERRAFAKAARDVPQEFVGDEAHIGDMVWSAAVPEELRSRAT
CCCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCHHHHHHHHH
AVIGNFPVSQASQYTRLEWLQTFSAGVDAYIRPGVLPRGTMVTNASGAYGQSVSEHMFAT
HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHH
MWALMKNLNRYASNQRDHQWRDEGPVLSPEGGIALIIGVGDIGSHFAQLAQSVGMRTFGV
HHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHCCCHHHCC
RRHADVPAKGIEKMYGFERLEALLPLADVVAMAVPRSPQTHHLLNADRLGRLKSTAIVIN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCCCCEEEEEE
AGRGDAIDHEALADALHNGRLHGAGLDVTEPEPLPAESPLWDEPHCLITPHVAGGNHLEK
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHH
TSEHIIRIALTNVSRYAKQQELLNLVRY
HHHHEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GIENTHKLIVNCLPLNEGERRAFAKAARDVPQEFVGDEAHIGDMVWSAAVPEELRSRAT
CCCCCEEEEEEEECCCCCHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCHHHHHHHHH
AVIGNFPVSQASQYTRLEWLQTFSAGVDAYIRPGVLPRGTMVTNASGAYGQSVSEHMFAT
HHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHH
MWALMKNLNRYASNQRDHQWRDEGPVLSPEGGIALIIGVGDIGSHFAQLAQSVGMRTFGV
HHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEECCHHHHHHHHHHHHCCCHHHCC
RRHADVPAKGIEKMYGFERLEALLPLADVVAMAVPRSPQTHHLLNADRLGRLKSTAIVIN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHCCCCCEEEEEE
AGRGDAIDHEALADALHNGRLHGAGLDVTEPEPLPAESPLWDEPHCLITPHVAGGNHLEK
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHH
TSEHIIRIALTNVSRYAKQQELLNLVRY
HHHHEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]