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The map label for this gene is lmrB [H]

Identifier: 213691613

GI number: 213691613

Start: 827225

End: 828682

Strand: Reverse

Name: lmrB [H]

Synonym: Blon_0717

Alternate gene names: 213691613

Gene position: 828682-827225 (Counterclockwise)

Preceding gene: 213691615

Following gene: 213691604

Centisome position: 29.25

GC content: 58.71

Gene sequence:

>1458_bases
ATGCCTGAGCAGCATATTGATAACGAGAAGATTCCCACACGTCTTATCGGCGCGATTGTGGCTGTCGGCTCACTTGCCTT
CATTGGCATTCTGACCGAAACCGTGATGACCGTGCTTTTCCCCGCACTGATGCGCGAATTCCATGTCGACACGGCCACTG
TGCAATGGATTACCACGATTTATCTGTTGGCGGTGGCCGCCACCATGCCCATCAGCTCGTTTTTGAAGCGCCGGCTCGCA
CTGAAAACCATTTTTCTTGCGGCTGTGATTCTGGCCATCGTTGGCTCGCTGATCATGATCGTCGCCCAAGCCTTCCCGCT
GCTGATTGTCGCCCGCATTATTCAGGGCATTGGCTCAGGCGTGGCCACGCCGTTGATGATCAACATCATTCTGGAGCAGT
CTCCTCGTTCGAAGATCGGCCGATTGATGGGCGTAGGATCGCTGGTCATTACCGTGGCTCCGGCCATCGGCCCGACCGTC
GGCGGCGCGGTGACCACAGTGCTGCCGTGGCGCGCGATCTTCGTCATCGCCATTCCGCTGGTGCTAATCGCAGCATTCAT
CGGCATGAAGTGCATTGAGCAGAAGACACCGACCGAAGCTGCCTATCTTGACCCGATTCAGCTTTGGTCCATTGTTGTCG
CGCTGGTCGGTCTGGTGCTGGCGCTGAACCAAGGTGGCGTGTCCATCAGTGCGGCGGTGTCCGGCAAGTCAGCCACACGC
TCCGGCATTATCGCCATTGTCAGTCTGATTGTGGGCCTCGCCTTGCTGATCGTGTTCGCCATGAGTTCCAAGCGTGCCTT
CTCCCCGCTGCTGCGCCTTGGCATTCTCAAGGATCCCGCCGTACTGTTACACGCCTGCGCATATCTGCTGCTGCCATTGG
TCGCCATCGGCTACGGCTATGTCATCACCAATGTGGCACAGCTCTCGCTCGGCACCACGGCATTTGTGGCCGGCTCTCTG
GTGCTGCCCGGAGCACTGGTCGGCGCTGTCTGTGCCCCGCTTGGCGGCTGGTTCTACGATAAGTTCGGCGCGGTCAAGCC
GATTCTGATTCCGATCGGCATCGCTATTTTGGGCCCAGTGCTTATGCTGGTGTTCTCGATGCAGCTTACGCCCGTGCTGC
TCGCCGGATTCTATTTCATTTTCGGTCTGTTCTATTCCATCGGCAATGCGAATGTGATGACCAGTGGCCTGTCGGAGGTC
TCTCCCGAGTTCAAGCCGGACGGCAACGCCATTTTCAACACGTGTCTTCAGTTTGGCGGCGCTGCCGGTACCGCACTGTT
CTCCACGATTCTTTCCGTGGCTCAGGCCGGCGCAGGAGAAGAAGGCACCGCTGAGTTTGCTCATGCCACTGCCGTCGGCG
GCACATGGACGTTTGCCACGATGACGGTTATCTGCCTGATTGGCTGGTGCTGTCTGGCCACCGCGTTCCGCATTCGCGCC
TCACGCCGGCGCGCCTAA

Upstream 100 bases:

>100_bases
CAAGGCAGTACGATTGCCGGTTTATCCGCACGCACAGGTACCTACGCCGGTTGGTAAACCTTACATTCCATACATTGCAA
CCGAACGTTTAGGAGCATGT

Downstream 100 bases:

>100_bases
GACCGTCAGTCCGCGGCCCGCGACGCCTCCCGCCCGGATTCGTCACCGTGCAGTATCGTGCGGATTCGGACGAGACGCTG
GCTGACTTCGTGTTCGTAGC

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MPEQHIDNEKIPTRLIGAIVAVGSLAFIGILTETVMTVLFPALMREFHVDTATVQWITTIYLLAVAATMPISSFLKRRLA
LKTIFLAAVILAIVGSLIMIVAQAFPLLIVARIIQGIGSGVATPLMINIILEQSPRSKIGRLMGVGSLVITVAPAIGPTV
GGAVTTVLPWRAIFVIAIPLVLIAAFIGMKCIEQKTPTEAAYLDPIQLWSIVVALVGLVLALNQGGVSISAAVSGKSATR
SGIIAIVSLIVGLALLIVFAMSSKRAFSPLLRLGILKDPAVLLHACAYLLLPLVAIGYGYVITNVAQLSLGTTAFVAGSL
VLPGALVGAVCAPLGGWFYDKFGAVKPILIPIGIAILGPVLMLVFSMQLTPVLLAGFYFIFGLFYSIGNANVMTSGLSEV
SPEFKPDGNAIFNTCLQFGGAAGTALFSTILSVAQAGAGEEGTAEFAHATAVGGTWTFATMTVICLIGWCCLATAFRIRA
SRRRA

Sequences:

>Translated_485_residues
MPEQHIDNEKIPTRLIGAIVAVGSLAFIGILTETVMTVLFPALMREFHVDTATVQWITTIYLLAVAATMPISSFLKRRLA
LKTIFLAAVILAIVGSLIMIVAQAFPLLIVARIIQGIGSGVATPLMINIILEQSPRSKIGRLMGVGSLVITVAPAIGPTV
GGAVTTVLPWRAIFVIAIPLVLIAAFIGMKCIEQKTPTEAAYLDPIQLWSIVVALVGLVLALNQGGVSISAAVSGKSATR
SGIIAIVSLIVGLALLIVFAMSSKRAFSPLLRLGILKDPAVLLHACAYLLLPLVAIGYGYVITNVAQLSLGTTAFVAGSL
VLPGALVGAVCAPLGGWFYDKFGAVKPILIPIGIAILGPVLMLVFSMQLTPVLLAGFYFIFGLFYSIGNANVMTSGLSEV
SPEFKPDGNAIFNTCLQFGGAAGTALFSTILSVAQAGAGEEGTAEFAHATAVGGTWTFATMTVICLIGWCCLATAFRIRA
SRRRA
>Mature_484_residues
PEQHIDNEKIPTRLIGAIVAVGSLAFIGILTETVMTVLFPALMREFHVDTATVQWITTIYLLAVAATMPISSFLKRRLAL
KTIFLAAVILAIVGSLIMIVAQAFPLLIVARIIQGIGSGVATPLMINIILEQSPRSKIGRLMGVGSLVITVAPAIGPTVG
GAVTTVLPWRAIFVIAIPLVLIAAFIGMKCIEQKTPTEAAYLDPIQLWSIVVALVGLVLALNQGGVSISAAVSGKSATRS
GIIAIVSLIVGLALLIVFAMSSKRAFSPLLRLGILKDPAVLLHACAYLLLPLVAIGYGYVITNVAQLSLGTTAFVAGSLV
LPGALVGAVCAPLGGWFYDKFGAVKPILIPIGIAILGPVLMLVFSMQLTPVLLAGFYFIFGLFYSIGNANVMTSGLSEVS
PEFKPDGNAIFNTCLQFGGAAGTALFSTILSVAQAGAGEEGTAEFAHATAVGGTWTFATMTVICLIGWCCLATAFRIRAS
RRRA

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=455, Percent_Identity=24.6153846153846, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI87081983, Length=461, Percent_Identity=24.7288503253796, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789042, Length=329, Percent_Identity=23.4042553191489, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788710, Length=327, Percent_Identity=23.2415902140673, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50677; Mature: 50545

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQHIDNEKIPTRLIGAIVAVGSLAFIGILTETVMTVLFPALMREFHVDTATVQWITTI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YLLAVAATMPISSFLKRRLALKTIFLAAVILAIVGSLIMIVAQAFPLLIVARIIQGIGSG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VATPLMINIILEQSPRSKIGRLMGVGSLVITVAPAIGPTVGGAVTTVLPWRAIFVIAIPL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VLIAAFIGMKCIEQKTPTEAAYLDPIQLWSIVVALVGLVLALNQGGVSISAAVSGKSATR
HHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
SGIIAIVSLIVGLALLIVFAMSSKRAFSPLLRLGILKDPAVLLHACAYLLLPLVAIGYGY
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VITNVAQLSLGTTAFVAGSLVLPGALVGAVCAPLGGWFYDKFGAVKPILIPIGIAILGPV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LMLVFSMQLTPVLLAGFYFIFGLFYSIGNANVMTSGLSEVSPEFKPDGNAIFNTCLQFGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCCHHHHHHHHHHCC
AAGTALFSTILSVAQAGAGEEGTAEFAHATAVGGTWTFATMTVICLIGWCCLATAFRIRA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRRA
HHCCC
>Mature Secondary Structure 
PEQHIDNEKIPTRLIGAIVAVGSLAFIGILTETVMTVLFPALMREFHVDTATVQWITTI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
YLLAVAATMPISSFLKRRLALKTIFLAAVILAIVGSLIMIVAQAFPLLIVARIIQGIGSG
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VATPLMINIILEQSPRSKIGRLMGVGSLVITVAPAIGPTVGGAVTTVLPWRAIFVIAIPL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VLIAAFIGMKCIEQKTPTEAAYLDPIQLWSIVVALVGLVLALNQGGVSISAAVSGKSATR
HHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHH
SGIIAIVSLIVGLALLIVFAMSSKRAFSPLLRLGILKDPAVLLHACAYLLLPLVAIGYGY
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VITNVAQLSLGTTAFVAGSLVLPGALVGAVCAPLGGWFYDKFGAVKPILIPIGIAILGPV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LMLVFSMQLTPVLLAGFYFIFGLFYSIGNANVMTSGLSEVSPEFKPDGNAIFNTCLQFGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCCHHHHHHHHHHCC
AAGTALFSTILSVAQAGAGEEGTAEFAHATAVGGTWTFATMTVICLIGWCCLATAFRIRA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SRRRA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]