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The map label for this gene is gsiB [H]

Identifier: 213691546

GI number: 213691546

Start: 745512

End: 747080

Strand: Direct

Name: gsiB [H]

Synonym: Blon_0647

Alternate gene names: 213691546

Gene position: 745512-747080 (Clockwise)

Preceding gene: 213691544

Following gene: 213691547

Centisome position: 26.32

GC content: 61.25

Gene sequence:

>1569_bases
ATGGTCAAGATTAATGCAAGGGTCAGGGCGGCCGTGGCGGCCATCCTGGCCGGCGGCATGGTTGTCGCCATGGCCGGCTG
CGGCGGCTCGTCCGCCACGGCCGGCGCCACCGGCACCTCGATTCCAGACACGATCACGACGGAAGTGGCATACGCGAGCC
GTGACTTCATGCCGAACACGACGTCCGGAGCGCTGCCGATGTCGGCGAACTGGCATGTCGTCGAACCGCTGTACGCGCTT
GACTACACGACTTACAAGACATTCCCGGCGCTCGCCAAAGGCGCGCCTCAGAAGGTCTCCGACACCGAGTACGTGATCAC
GCTGCGTGACGGCGCGAAGTTCTCCGATGGCACGCCCGTCAAGGCGTCCGATGTCGTGAGCTCCTACAAGCGCTCGACCG
CCGAAGGCAGCCTGTTCATCTCGATGCTCGACTTCATCGACTCCGTCGAGGCGAAGGGCGACAACCAGGTGGACTTCAAG
CTCAAGGAAGCCTTCCCGATGTTCGAAAAGCGTCTGGCGCTCATCCCGATCGTCCCGTCCGGCAAGTCGGACGCCGAACT
CAAGAAGATGCCCACCGGTTCCGGGCCGTGGAAGTACACGGCGATCACCGACCGGCAGGTGAGCTTCGAGAAGAACGACG
CCTACAACGGCAAGTATCCGGCCCAGACAAAGAAGATGGTCTGGAACGTCAACGTCGACGACACCGCGCGAGTCACCGCG
ATGCAGGCCGGCAAGACGGACGTCATGGAAAACGTGCCCGCGAAGGCGGTGCAGACGCTGACGTCCTCGGGTTCCGACAT
CCAGACGGTGCAGGGCTTCAACCAGGCGTTCATCATGTTCAACACGAAGAAGAAGCCGTTCGACGACCCCCGCGTGCGTC
AGGCCGTGTTCTACTCGATCGACACGAAGAAGCTCATCGACAACCAGCTGTCCGGACAGGCGCAGACCGTCACGAGTTTC
CTGCCCGAGAACTTCGCGAACTACCATAAGGCGAAGAACGTCTACACGAAGAACACCGCGAAGGCCAAGGAACTGCTGAA
GGAAGCAGGCGTCAACGGTCCGATCAAGACCACGCTGTACACGACCGACCAGACCTGGGTCACGCAGCTCGCCCCACAGA
TCAAGAACGATCTTGCGGAATCAGGCATCGACGTGGATATCCAGTCGATGAAGTCGTCGGCGTTGTATCCGTCGATCACC
GACAAGGACGGCGCTGACTTCTCGATGGTTCTCGCCCCGGGCGATCCCTCGGTCTTCGGCAACGACCCCGACCTGCTGAT
GAACTGGTGGTACGGAGACAACGTCTGGACCAAGCAGCGCACGTTCTGGAAGGGGTCGGAAGGCTATGACGAGCTGCACG
ACCTGATGGGCAAGGCCCTGTCCGCGTCGAGCGATGCCGAGCGCCAGGACTACTGGAACCAGTGCTTCGATCTGCTTTCC
GAGCAGGTGCCGCTTTATCCGCTCTTCCACCGCAAGGTGAGCACGGCCGTCAAGAAGGGTGTCTTCGACGAATACAAGAC
GATGGGCACGACGGGTCTCAACCTGGTCCAGGCCAAAGTCGCACAGTGA

Upstream 100 bases:

>100_bases
CACGGCGGATTGGCGGTCCGGCCGGCCTGGACGATTGTCGATGTCGACCATCGTCGAGTTGGACGCACGATGACACATTC
TCAACGATGAGGAGATCTCA

Downstream 100 bases:

>100_bases
GCCACGCAAAGGAGCGATGGCGCAGAACCCTTCCCTGACGATCTGATACCAAGACAGGCCCGTTCATCTCGTTCCCCTTC
CCTCCGCGATCCGTGGACGG

Product: extracellular solute-binding protein, family 5

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MVKINARVRAAVAAILAGGMVVAMAGCGGSSATAGATGTSIPDTITTEVAYASRDFMPNTTSGALPMSANWHVVEPLYAL
DYTTYKTFPALAKGAPQKVSDTEYVITLRDGAKFSDGTPVKASDVVSSYKRSTAEGSLFISMLDFIDSVEAKGDNQVDFK
LKEAFPMFEKRLALIPIVPSGKSDAELKKMPTGSGPWKYTAITDRQVSFEKNDAYNGKYPAQTKKMVWNVNVDDTARVTA
MQAGKTDVMENVPAKAVQTLTSSGSDIQTVQGFNQAFIMFNTKKKPFDDPRVRQAVFYSIDTKKLIDNQLSGQAQTVTSF
LPENFANYHKAKNVYTKNTAKAKELLKEAGVNGPIKTTLYTTDQTWVTQLAPQIKNDLAESGIDVDIQSMKSSALYPSIT
DKDGADFSMVLAPGDPSVFGNDPDLLMNWWYGDNVWTKQRTFWKGSEGYDELHDLMGKALSASSDAERQDYWNQCFDLLS
EQVPLYPLFHRKVSTAVKKGVFDEYKTMGTTGLNLVQAKVAQ

Sequences:

>Translated_522_residues
MVKINARVRAAVAAILAGGMVVAMAGCGGSSATAGATGTSIPDTITTEVAYASRDFMPNTTSGALPMSANWHVVEPLYAL
DYTTYKTFPALAKGAPQKVSDTEYVITLRDGAKFSDGTPVKASDVVSSYKRSTAEGSLFISMLDFIDSVEAKGDNQVDFK
LKEAFPMFEKRLALIPIVPSGKSDAELKKMPTGSGPWKYTAITDRQVSFEKNDAYNGKYPAQTKKMVWNVNVDDTARVTA
MQAGKTDVMENVPAKAVQTLTSSGSDIQTVQGFNQAFIMFNTKKKPFDDPRVRQAVFYSIDTKKLIDNQLSGQAQTVTSF
LPENFANYHKAKNVYTKNTAKAKELLKEAGVNGPIKTTLYTTDQTWVTQLAPQIKNDLAESGIDVDIQSMKSSALYPSIT
DKDGADFSMVLAPGDPSVFGNDPDLLMNWWYGDNVWTKQRTFWKGSEGYDELHDLMGKALSASSDAERQDYWNQCFDLLS
EQVPLYPLFHRKVSTAVKKGVFDEYKTMGTTGLNLVQAKVAQ
>Mature_522_residues
MVKINARVRAAVAAILAGGMVVAMAGCGGSSATAGATGTSIPDTITTEVAYASRDFMPNTTSGALPMSANWHVVEPLYAL
DYTTYKTFPALAKGAPQKVSDTEYVITLRDGAKFSDGTPVKASDVVSSYKRSTAEGSLFISMLDFIDSVEAKGDNQVDFK
LKEAFPMFEKRLALIPIVPSGKSDAELKKMPTGSGPWKYTAITDRQVSFEKNDAYNGKYPAQTKKMVWNVNVDDTARVTA
MQAGKTDVMENVPAKAVQTLTSSGSDIQTVQGFNQAFIMFNTKKKPFDDPRVRQAVFYSIDTKKLIDNQLSGQAQTVTSF
LPENFANYHKAKNVYTKNTAKAKELLKEAGVNGPIKTTLYTTDQTWVTQLAPQIKNDLAESGIDVDIQSMKSSALYPSIT
DKDGADFSMVLAPGDPSVFGNDPDLLMNWWYGDNVWTKQRTFWKGSEGYDELHDLMGKALSASSDAERQDYWNQCFDLLS
EQVPLYPLFHRKVSTAVKKGVFDEYKTMGTTGLNLVQAKVAQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=411, Percent_Identity=24.5742092457421, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789887, Length=437, Percent_Identity=27.9176201372998, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1789966, Length=455, Percent_Identity=23.2967032967033, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787762, Length=415, Percent_Identity=22.8915662650602, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787551, Length=447, Percent_Identity=24.8322147651007, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789397, Length=438, Percent_Identity=21.689497716895, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57155; Mature: 57155

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKINARVRAAVAAILAGGMVVAMAGCGGSSATAGATGTSIPDTITTEVAYASRDFMPNT
CEEECHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
TSGALPMSANWHVVEPLYALDYTTYKTFPALAKGAPQKVSDTEYVITLRDGAKFSDGTPV
CCCCCCCCCCCEEECCHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCC
KASDVVSSYKRSTAEGSLFISMLDFIDSVEAKGDNQVDFKLKEAFPMFEKRLALIPIVPS
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCEEEEEECCC
GKSDAELKKMPTGSGPWKYTAITDRQVSFEKNDAYNGKYPAQTKKMVWNVNVDDTARVTA
CCCCCHHHHCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
MQAGKTDVMENVPAKAVQTLTSSGSDIQTVQGFNQAFIMFNTKKKPFDDPRVRQAVFYSI
ECCCCHHHHHCCCHHHHHHHHCCCCCCHHHCCCCEEEEEEECCCCCCCCHHHHHHHEEEC
DTKKLIDNQLSGQAQTVTSFLPENFANYHKAKNVYTKNTAKAKELLKEAGVNGPIKTTLY
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
TTDQTWVTQLAPQIKNDLAESGIDVDIQSMKSSALYPSITDKDGADFSMVLAPGDPSVFG
ECCHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
NDPDLLMNWWYGDNVWTKQRTFWKGSEGYDELHDLMGKALSASSDAERQDYWNQCFDLLS
CCCCEEEEEEECCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EQVPLYPLFHRKVSTAVKKGVFDEYKTMGTTGLNLVQAKVAQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
>Mature Secondary Structure
MVKINARVRAAVAAILAGGMVVAMAGCGGSSATAGATGTSIPDTITTEVAYASRDFMPNT
CEEECHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
TSGALPMSANWHVVEPLYALDYTTYKTFPALAKGAPQKVSDTEYVITLRDGAKFSDGTPV
CCCCCCCCCCCEEECCHHHHCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCC
KASDVVSSYKRSTAEGSLFISMLDFIDSVEAKGDNQVDFKLKEAFPMFEKRLALIPIVPS
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCEEEEEECCC
GKSDAELKKMPTGSGPWKYTAITDRQVSFEKNDAYNGKYPAQTKKMVWNVNVDDTARVTA
CCCCCHHHHCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEE
MQAGKTDVMENVPAKAVQTLTSSGSDIQTVQGFNQAFIMFNTKKKPFDDPRVRQAVFYSI
ECCCCHHHHHCCCHHHHHHHHCCCCCCHHHCCCCEEEEEEECCCCCCCCHHHHHHHEEEC
DTKKLIDNQLSGQAQTVTSFLPENFANYHKAKNVYTKNTAKAKELLKEAGVNGPIKTTLY
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
TTDQTWVTQLAPQIKNDLAESGIDVDIQSMKSSALYPSITDKDGADFSMVLAPGDPSVFG
ECCHHHHHHHHHHHHHHHHHCCCCEEHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCC
NDPDLLMNWWYGDNVWTKQRTFWKGSEGYDELHDLMGKALSASSDAERQDYWNQCFDLLS
CCCCEEEEEEECCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
EQVPLYPLFHRKVSTAVKKGVFDEYKTMGTTGLNLVQAKVAQ
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA