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The map label for this gene is lacR [H]

Identifier: 213691540

GI number: 213691540

Start: 738087

End: 739097

Strand: Direct

Name: lacR [H]

Synonym: Blon_0641

Alternate gene names: 213691540

Gene position: 738087-739097 (Clockwise)

Preceding gene: 213691539

Following gene: 213691541

Centisome position: 26.06

GC content: 61.82

Gene sequence:

>1011_bases
ATGGTCACGATGAAGGAAATCGCACAGAGATCAGGCTTCTCGCAGGCCACGGTATCCCGGCTGCTCAATGGGGACCCGAC
ATTGTCCGTCAAAGAGGAGACGCGACGGCGCATCATCGAGGTCAGCGAGCAGCTTGGCTACGCTACCGGATCACGCCGTA
TCGCATTGCCACGCCGTGTGGCCGTACTCGACAACGCCACCCGCGAGGAAGAACTGGCGGACGCCTATTTCGATGAACTG
CGGTCCGTGCTTGGCGACAATGCCAGAGCCCAGCACATGGAACTCACGTCGTTTTCCCATATCGACAATCTGATTGCCAA
CGCCGGTGATTTCGACGGCTTCATCTCCATCGGACCGTCCGTACTCTCTTCGGATGGACTGTTGAAGCTCCACCTGGCAT
TGCCCCATGGCGTATTCATCGACATCAACCCGGCGCCCAGCCTGTTCGATTCGGTACAGCCGGACCTGGGGCAGACCATC
CTGGAGGCCTTGGACGTGCTGATGGCCTCCGGCAAGCGCCGCATCGGCTACATCGGCGGCGCCGGCTTCACGATGGGCCT
GCACGAATACCCCGAAGACGGCCGTCTGACCGCATTCCGCAACTGGACCGAGCGTCTGGGGCTGGATACGGAGGGGCTGA
TCTATGCGCAGGGCGCCTTCACCGTGGACACCGGACGTGTCCTAGGCCATCAGGCGGTCAAGGACCACCGCGACGACATG
CCGGATGCGTTCATCGTGGCCGCCGATTCGATCGCGGTCGGCGTATTGCAGTCGTTCACCGCGGCGGGCGTGTTGGTACC
GCGCGACACCAGCGTGATCAGCATCAACAACCAGTCCATCGCCCAATACACCTCGCCGACGCTGACCAGCTACGACATCG
ACCAGAACGAACTGGCCGACACGGCGATCACGATGCTCGCCGAGGCCATCTCCAGCAAACGCACGTTGCGGCACCACACG
TTCATCTCCACCACCCTCGTGGTGCGGGACAGTTTCGTTCCCGTGCGATGA

Upstream 100 bases:

>100_bases
TTCTCAATAGACAGCGGGATGACGTGAGTTACACTATGTAGTAAAGTAATTTACTGAAATACTGCGTATTGTTACGTACT
GAGGCGTGGAAGGCTCAACT

Downstream 100 bases:

>100_bases
GCGTACGGACGGCCCCGCAACGCACGGCTGCGTGGTTGCCGATGGGGCGGTGCGGGCCTATGCTGGTGCGTGAAACGTCT
GTCAGGGGCGGTTAATGTCG

Product: regulatory protein, LacI

Products: NA

Alternate protein names: Lactose operon repressor [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MVTMKEIAQRSGFSQATVSRLLNGDPTLSVKEETRRRIIEVSEQLGYATGSRRIALPRRVAVLDNATREEELADAYFDEL
RSVLGDNARAQHMELTSFSHIDNLIANAGDFDGFISIGPSVLSSDGLLKLHLALPHGVFIDINPAPSLFDSVQPDLGQTI
LEALDVLMASGKRRIGYIGGAGFTMGLHEYPEDGRLTAFRNWTERLGLDTEGLIYAQGAFTVDTGRVLGHQAVKDHRDDM
PDAFIVAADSIAVGVLQSFTAAGVLVPRDTSVISINNQSIAQYTSPTLTSYDIDQNELADTAITMLAEAISSKRTLRHHT
FISTTLVVRDSFVPVR

Sequences:

>Translated_336_residues
MVTMKEIAQRSGFSQATVSRLLNGDPTLSVKEETRRRIIEVSEQLGYATGSRRIALPRRVAVLDNATREEELADAYFDEL
RSVLGDNARAQHMELTSFSHIDNLIANAGDFDGFISIGPSVLSSDGLLKLHLALPHGVFIDINPAPSLFDSVQPDLGQTI
LEALDVLMASGKRRIGYIGGAGFTMGLHEYPEDGRLTAFRNWTERLGLDTEGLIYAQGAFTVDTGRVLGHQAVKDHRDDM
PDAFIVAADSIAVGVLQSFTAAGVLVPRDTSVISINNQSIAQYTSPTLTSYDIDQNELADTAITMLAEAISSKRTLRHHT
FISTTLVVRDSFVPVR
>Mature_336_residues
MVTMKEIAQRSGFSQATVSRLLNGDPTLSVKEETRRRIIEVSEQLGYATGSRRIALPRRVAVLDNATREEELADAYFDEL
RSVLGDNARAQHMELTSFSHIDNLIANAGDFDGFISIGPSVLSSDGLLKLHLALPHGVFIDINPAPSLFDSVQPDLGQTI
LEALDVLMASGKRRIGYIGGAGFTMGLHEYPEDGRLTAFRNWTERLGLDTEGLIYAQGAFTVDTGRVLGHQAVKDHRDDM
PDAFIVAADSIAVGVLQSFTAAGVLVPRDTSVISINNQSIAQYTSPTLTSYDIDQNELADTAITMLAEAISSKRTLRHHT
FISTTLVVRDSFVPVR

Specific function: Negatively regulates the transcription of the lactose utilization genes lacL and lacM [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=339, Percent_Identity=30.9734513274336, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1790369, Length=349, Percent_Identity=26.9340974212034, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789068, Length=361, Percent_Identity=25.4847645429363, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00356 LacI [H]

EC number: NA

Molecular weight: Translated: 36631; Mature: 36631

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTMKEIAQRSGFSQATVSRLLNGDPTLSVKEETRRRIIEVSEQLGYATGSRRIALPRRV
CCCHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEECCHHH
AVLDNATREEELADAYFDELRSVLGDNARAQHMELTSFSHIDNLIANAGDFDGFISIGPS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHHHHHHCCCCCCCCEECCHH
VLSSDGLLKLHLALPHGVFIDINPAPSLFDSVQPDLGQTILEALDVLMASGKRRIGYIGG
HHCCCCEEEEEEECCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECC
AGFTMGLHEYPEDGRLTAFRNWTERLGLDTEGLIYAQGAFTVDTGRVLGHQAVKDHRDDM
CCEECCHHCCCCCCCEEHHHHHHHHHCCCCCCEEEECCEEEEECCHHHHHHHHHHHHCCC
PDAFIVAADSIAVGVLQSFTAAGVLVPRDTSVISINNQSIAQYTSPTLTSYDIDQNELAD
CCEEEEEEHHHHHHHHHHHHHCCEEEECCCCEEEECCCCHHHHCCCCCEEECCCHHHHHH
TAITMLAEAISSKRTLRHHTFISTTLVVRDSFVPVR
HHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCC
>Mature Secondary Structure
MVTMKEIAQRSGFSQATVSRLLNGDPTLSVKEETRRRIIEVSEQLGYATGSRRIALPRRV
CCCHHHHHHHCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCEEECCHHH
AVLDNATREEELADAYFDELRSVLGDNARAQHMELTSFSHIDNLIANAGDFDGFISIGPS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEHHHHHHHHHHHHCCCCCCCCEECCHH
VLSSDGLLKLHLALPHGVFIDINPAPSLFDSVQPDLGQTILEALDVLMASGKRRIGYIGG
HHCCCCEEEEEEECCCCEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECC
AGFTMGLHEYPEDGRLTAFRNWTERLGLDTEGLIYAQGAFTVDTGRVLGHQAVKDHRDDM
CCEECCHHCCCCCCCEEHHHHHHHHHCCCCCCEEEECCEEEEECCHHHHHHHHHHHHCCC
PDAFIVAADSIAVGVLQSFTAAGVLVPRDTSVISINNQSIAQYTSPTLTSYDIDQNELAD
CCEEEEEEHHHHHHHHHHHHHCCEEEECCCCEEEECCCCHHHHCCCCCEEECCCHHHHHH
TAITMLAEAISSKRTLRHHTFISTTLVVRDSFVPVR
HHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788721 [H]