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The map label for this gene is rihB [H]

Identifier: 213691537

GI number: 213691537

Start: 734195

End: 735133

Strand: Direct

Name: rihB [H]

Synonym: Blon_0638

Alternate gene names: 213691537

Gene position: 734195-735133 (Clockwise)

Preceding gene: 213691536

Following gene: 213691538

Centisome position: 25.92

GC content: 58.47

Gene sequence:

>939_bases
ATGGAAAAAATTATTCTGGATTGCGATCCCGGTCATGATGATGCCATGGCCATCATGCTTGCTGTAGGCAACCCCAAAAT
TGACCTAGTGGGTGTGACCACGGTGGGTGGCAATCAGAGCCTTGAAAAGGTGACGTACAACGCCCGGTCCGTTCTGGAAA
TGGCGAAGGCCACGGACATCCCGGTGCACGCCGGTTGCGATAGGCCCCTCGTCCGCAAGCAGGAGGTCGCCGCATCGATT
CACGGCGAGACCGGTTTGGATGGCGTTGAATTGCCGGTTCCCACCCGTCCACTGGATTCCGGCCATGCCGTGAACTGGAT
TATCGATACGATTATGGCCAGCGAGCCGGGAACAATCACGCTTGTGCCGACCGGCCCTCTGACCAACATCGCCATGGCCG
CCCGTATGGAGCCGCGCATCGTAGACCGCGTCAAGGAAGTCGTCCTGATGGGCGGCGGATACCACGTCGGCAATTGGAGC
GCCGTAGCGGAATTCAACATCAAGGTGGATCCCGAGGCCGCGCACATCGTGTTCAACGAGCCGTGGAAGCTAACCATGGT
TGGCCTTGATCTGACCCATCAGGCGCTGTGCACTCCGGAGGTTCAGCATCGCATTGATGCCATCGGCACTGATTCGTCGC
ACTTCGCCAGCGGACTTATGGACTTCTTCCGCAAGACCTATCGGGATAATCAGGACTTCATCGATCCGCCGGTGCATGAT
CCCTGCACGGTCGCCTATCTGATTGATCCGAGCGTGGTGTCGACTCGCCGTTGCCCGCTGGATGTCGAAATCCACGGTGA
CCTGACACTGGGCATGACCGTTGCCGACCTGCGTGGCCCGGAACCGTCCGCCGAGGAATGCCATACGCAGGTCGCGGTGA
AACTCGACTTCGATAAGTTCTGGGATCTCATTGTCGATGCCATCGTTGCTATCGGCTGA

Upstream 100 bases:

>100_bases
GATTTAACGTCTTATCATCAAATAAGCTGATGATGAAACGTTAAATCATCAGAAGTCAGAGATGACCCAGCGGCTGTTAT
GGAAGCAAAGGAGCACTTTG

Downstream 100 bases:

>100_bases
CGGGCGAAGAGGAATGCGTAAAAGGACAAGGGAATAGTCATGTCGACAGTCAGATCCGAAAAAATGCTTGATGAGGGCGG
CAAGTCCATTGTCGCTTTGA

Product: Purine nucleosidase

Products: NA

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MEKIILDCDPGHDDAMAIMLAVGNPKIDLVGVTTVGGNQSLEKVTYNARSVLEMAKATDIPVHAGCDRPLVRKQEVAASI
HGETGLDGVELPVPTRPLDSGHAVNWIIDTIMASEPGTITLVPTGPLTNIAMAARMEPRIVDRVKEVVLMGGGYHVGNWS
AVAEFNIKVDPEAAHIVFNEPWKLTMVGLDLTHQALCTPEVQHRIDAIGTDSSHFASGLMDFFRKTYRDNQDFIDPPVHD
PCTVAYLIDPSVVSTRRCPLDVEIHGDLTLGMTVADLRGPEPSAEECHTQVAVKLDFDKFWDLIVDAIVAIG

Sequences:

>Translated_312_residues
MEKIILDCDPGHDDAMAIMLAVGNPKIDLVGVTTVGGNQSLEKVTYNARSVLEMAKATDIPVHAGCDRPLVRKQEVAASI
HGETGLDGVELPVPTRPLDSGHAVNWIIDTIMASEPGTITLVPTGPLTNIAMAARMEPRIVDRVKEVVLMGGGYHVGNWS
AVAEFNIKVDPEAAHIVFNEPWKLTMVGLDLTHQALCTPEVQHRIDAIGTDSSHFASGLMDFFRKTYRDNQDFIDPPVHD
PCTVAYLIDPSVVSTRRCPLDVEIHGDLTLGMTVADLRGPEPSAEECHTQVAVKLDFDKFWDLIVDAIVAIG
>Mature_312_residues
MEKIILDCDPGHDDAMAIMLAVGNPKIDLVGVTTVGGNQSLEKVTYNARSVLEMAKATDIPVHAGCDRPLVRKQEVAASI
HGETGLDGVELPVPTRPLDSGHAVNWIIDTIMASEPGTITLVPTGPLTNIAMAARMEPRIVDRVKEVVLMGGGYHVGNWS
AVAEFNIKVDPEAAHIVFNEPWKLTMVGLDLTHQALCTPEVQHRIDAIGTDSSHFASGLMDFFRKTYRDNQDFIDPPVHD
PCTVAYLIDPSVVSTRRCPLDVEIHGDLTLGMTVADLRGPEPSAEECHTQVAVKLDFDKFWDLIVDAIVAIG

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=46.2540716612378, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=41.9354838709677, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786213, Length=312, Percent_Identity=36.5384615384615, Blast_Score=158, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI32565474, Length=215, Percent_Identity=36.2790697674419, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17565698, Length=320, Percent_Identity=28.4375, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=325, Percent_Identity=37.2307692307692, Blast_Score=176, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24641837, Length=186, Percent_Identity=38.7096774193548, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24641839, Length=302, Percent_Identity=29.8013245033113, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24583915, Length=187, Percent_Identity=31.0160427807487, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022977 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: =3.2.2.8 [H]

Molecular weight: Translated: 33870; Mature: 33870

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIILDCDPGHDDAMAIMLAVGNPKIDLVGVTTVGGNQSLEKVTYNARSVLEMAKATDI
CCEEEEECCCCCCCEEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
PVHAGCDRPLVRKQEVAASIHGETGLDGVELPVPTRPLDSGHAVNWIIDTIMASEPGTIT
CEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
LVPTGPLTNIAMAARMEPRIVDRVKEVVLMGGGYHVGNWSAVAEFNIKVDPEAAHIVFNE
EEECCCCHHHHHHHCCCCHHHHHHHHHEEECCCEEECCCEEEEEEEEEECCCEEEEEECC
PWKLTMVGLDLTHQALCTPEVQHRIDAIGTDSSHFASGLMDFFRKTYRDNQDFIDPPVHD
CEEEEEEEECCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PCTVAYLIDPSVVSTRRCPLDVEIHGDLTLGMTVADLRGPEPSAEECHTQVAVKLDFDKF
CEEEEEEECCCHHCCCCCCEEEEEECCEEEEEEEEECCCCCCCHHHHCEEEEEEECHHHH
WDLIVDAIVAIG
HHHHHHHHHHCC
>Mature Secondary Structure
MEKIILDCDPGHDDAMAIMLAVGNPKIDLVGVTTVGGNQSLEKVTYNARSVLEMAKATDI
CCEEEEECCCCCCCEEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
PVHAGCDRPLVRKQEVAASIHGETGLDGVELPVPTRPLDSGHAVNWIIDTIMASEPGTIT
CEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
LVPTGPLTNIAMAARMEPRIVDRVKEVVLMGGGYHVGNWSAVAEFNIKVDPEAAHIVFNE
EEECCCCHHHHHHHCCCCHHHHHHHHHEEECCCEEECCCEEEEEEEEEECCCEEEEEECC
PWKLTMVGLDLTHQALCTPEVQHRIDAIGTDSSHFASGLMDFFRKTYRDNQDFIDPPVHD
CEEEEEEEECCCCHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PCTVAYLIDPSVVSTRRCPLDVEIHGDLTLGMTVADLRGPEPSAEECHTQVAVKLDFDKF
CEEEEEEECCCHHCCCCCCEEEEEECCEEEEEEEEECCCCCCCHHHHCEEEEEEECHHHH
WDLIVDAIVAIG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA