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The map label for this gene is insK [C]

Identifier: 213691521

GI number: 213691521

Start: 709530

End: 710885

Strand: Direct

Name: insK [C]

Synonym: Blon_0622

Alternate gene names: 213691521

Gene position: 709530-710885 (Clockwise)

Preceding gene: 213691520

Following gene: 213691522

Centisome position: 25.05

GC content: 66.08

Gene sequence:

>1356_bases
ATGTTTTCGATGGAGGATCGGCGCAGGGCCGTTGACCTGTATTTCACGGAGGGCATGACCATCAGGAAGGTCATTGCCGA
GCTTGGTTACCCGTCCGAGGGCGCGCTGGTGAAATGGGTTCGCGAGGACCCGCGTTACACGGGCGCGTGCAGGCGTTCGT
ATACGCTGGAATGCAAGACGAATGCGGCCAGACGCGCGCTCGAGGGCGAGCCCCTCGCCCGCGTGGCGCGCGACGCGGGT
TGCACGCCGACGAGCGTGTACCAGTGGATGCGCCGCTACCGGAGCGAGGGGATACTGGGACTGATGGACAGGAGAAACGC
GCCGATGCCGGCCGAACCGATGCCCGCGGCCGGCGATGACGTGGAGGAGCTGCGCCGGCAGGTGGAGGTCCTGCGGCTGG
AGAACGCGGTGATGCGGGAGACGATCGATGTTTTAAAAGCCGACGACCCGCGCCTCGACCCGTCCATGCTGACGAACAGG
GAGAGGACGCGGGTCGTCGACGCGATCAGGGGTGAATTCGGCCTGGCGGCCTGTCTGAAGGCCGTGGGGCTGAAACGCAG
CACCTACTATTACGAGCGCGGCGCGATCGCCGCGGGCGACAGGTACGCCGTGCTGCGGGCCCGCGTCGCCGGCCTGTTCG
AGCAGGGTGGGCGCGCATGGGGATACCGGACCATCCACCGCATGCTGCGCCTTGACGAGTCCGACCCGATCGTCGTCTCG
GAGAAGGTCGTCAGGCGGATCATGCGCGAGGGGGCCATGCGGCCCGTGTACCTGAAGCGGCCGAAGCGGTGGAGCTCCTA
CGCCGGCGAGATCGGCGAGGCCCCGGCGAACCTCGTGGAGCGGGACTTCCACGCCGACGCGCCGAACATGCTGTGGGTCA
CGGACGTCACCCAGTTCACCATGGACGGGTACAAGTGCTGGCTTTCCCCGGTGGTCGACTGCTTCGACGGGATGGTGGTC
TCCTGGACCTTGTCGCGCTCCCCGAACGCCGACATGGCGAACCGGATGCTCCTTGACGCGGTCGCCACGCTCAGGGACGG
GGAACATCCAATCATCCATTCCGACCGCGGCTGCCACTACCGGTGGGACGAATGGATCCGCATCTGCGAGGAGCACGGGC
TGATCCGGTCGATGAGCGCCAAGGGATGCAGCCCGGACAACGCGGCCGCCGAGGGGTTCTTCGGCAGGCTCAAGAACGAG
TTATTCTACGGGCGGGACTGGAGGGGCGTGGGCTACGAGGAGTTCCGCGAACGCCTGGCCGCCTACCTGACCCACTACAA
TGAAACCAGGATCAAGAAGTCACTGGACTGGATGAGCCCCGTGCAATACCGCAGAAGTCTTGGACTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCAACAAGAATTCACTGCGAAGATGGTTTGGCTCTTGTCATAGTTTGGGAGGGGATGCGGACGGAAAGTTGGACGCTTGG
GCGTCCGGATTGGAGTCCGC

Downstream 100 bases:

>100_bases
CCGTCCAAAAAAACATCCGCACCCCCGGATGAGGGAAATTTTGCTCATTTATGTGGCTTGGATTATTATCTGGATGATTC
CAAGCGACGCCGTCTTCCTC

Product: Integrase, catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MFSMEDRRRAVDLYFTEGMTIRKVIAELGYPSEGALVKWVREDPRYTGACRRSYTLECKTNAARRALEGEPLARVARDAG
CTPTSVYQWMRRYRSEGILGLMDRRNAPMPAEPMPAAGDDVEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNR
ERTRVVDAIRGEFGLAACLKAVGLKRSTYYYERGAIAAGDRYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVS
EKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFTMDGYKCWLSPVVDCFDGMVV
SWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHYRWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNE
LFYGRDWRGVGYEEFRERLAAYLTHYNETRIKKSLDWMSPVQYRRSLGLAA

Sequences:

>Translated_451_residues
MFSMEDRRRAVDLYFTEGMTIRKVIAELGYPSEGALVKWVREDPRYTGACRRSYTLECKTNAARRALEGEPLARVARDAG
CTPTSVYQWMRRYRSEGILGLMDRRNAPMPAEPMPAAGDDVEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNR
ERTRVVDAIRGEFGLAACLKAVGLKRSTYYYERGAIAAGDRYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVS
EKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFTMDGYKCWLSPVVDCFDGMVV
SWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHYRWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNE
LFYGRDWRGVGYEEFRERLAAYLTHYNETRIKKSLDWMSPVQYRRSLGLAA
>Mature_451_residues
MFSMEDRRRAVDLYFTEGMTIRKVIAELGYPSEGALVKWVREDPRYTGACRRSYTLECKTNAARRALEGEPLARVARDAG
CTPTSVYQWMRRYRSEGILGLMDRRNAPMPAEPMPAAGDDVEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNR
ERTRVVDAIRGEFGLAACLKAVGLKRSTYYYERGAIAAGDRYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVS
EKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFTMDGYKCWLSPVVDCFDGMVV
SWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHYRWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNE
LFYGRDWRGVGYEEFRERLAAYLTHYNETRIKKSLDWMSPVQYRRSLGLAA

Specific function: Probably involved in the transposition of insertion sequence IS1353 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=257, Percent_Identity=26.4591439688716, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1789981, Length=281, Percent_Identity=36.2989323843416, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788405, Length=168, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787263, Length=168, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786753, Length=168, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786570, Length=168, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786490, Length=168, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR002514 [H]

Pfam domain/function: PF00665 rve; PF01527 Transposase_8 [H]

EC number: NA

Molecular weight: Translated: 51711; Mature: 51711

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSMEDRRRAVDLYFTEGMTIRKVIAELGYPSEGALVKWVREDPRYTGACRRSYTLECKT
CCCCCCHHHEEEEEEECCCHHHHHHHHCCCCCCCCEEHHHHHCCCCCCHHCCCEEEEECC
NAARRALEGEPLARVARDAGCTPTSVYQWMRRYRSEGILGLMDRRNAPMPAEPMPAAGDD
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
VEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNRERTRVVDAIRGEFGLAACLK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCHHHHHHHH
AVGLKRSTYYYERGAIAAGDRYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVS
HHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEC
EKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFT
HHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHH
MDGYKCWLSPVVDCFDGMVVSWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHY
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
RWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNELFYGRDWRGVGYEEFRERLA
CHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AYLTHYNETRIKKSLDWMSPVQYRRSLGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFSMEDRRRAVDLYFTEGMTIRKVIAELGYPSEGALVKWVREDPRYTGACRRSYTLECKT
CCCCCCHHHEEEEEEECCCHHHHHHHHCCCCCCCCEEHHHHHCCCCCCHHCCCEEEEECC
NAARRALEGEPLARVARDAGCTPTSVYQWMRRYRSEGILGLMDRRNAPMPAEPMPAAGDD
HHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCC
VEELRRQVEVLRLENAVMRETIDVLKADDPRLDPSMLTNRERTRVVDAIRGEFGLAACLK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHCCHHHHHHHH
AVGLKRSTYYYERGAIAAGDRYAVLRARVAGLFEQGGRAWGYRTIHRMLRLDESDPIVVS
HHCCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEC
EKVVRRIMREGAMRPVYLKRPKRWSSYAGEIGEAPANLVERDFHADAPNMLWVTDVTQFT
HHHHHHHHHHCCCCCEEECCCCHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEECHHHHH
MDGYKCWLSPVVDCFDGMVVSWTLSRSPNADMANRMLLDAVATLRDGEHPIIHSDRGCHY
HHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
RWDEWIRICEEHGLIRSMSAKGCSPDNAAAEGFFGRLKNELFYGRDWRGVGYEEFRERLA
CHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
AYLTHYNETRIKKSLDWMSPVQYRRSLGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8755869; 12499211 [H]