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The map label for this gene is prfA [H]

Identifier: 213691478

GI number: 213691478

Start: 649886

End: 650974

Strand: Direct

Name: prfA [H]

Synonym: Blon_0575

Alternate gene names: 213691478

Gene position: 649886-650974 (Clockwise)

Preceding gene: 213691477

Following gene: 213691479

Centisome position: 22.94

GC content: 63.27

Gene sequence:

>1089_bases
ATGGCAGACGAACAGTTCCCGGCGGCGGCAACCGCGCTTGAGGAGTACCAGTCCATCGAAGAGCAGATGGCGAGCCCCGA
AGTCGTGTCCAACCCGGACAAGCTGCGCAAGCTGGGCCGTCGTCACGCCGAACTGGGTGCCATCGTGGGTGCCTACAAAA
CCTGGTTGCAGGTGAAGGACGATCTGGCAGCCGCTCAGGAGATGGCCGGCGAAGACGCCGACTTCGCCGAGGAAGCCAAG
CGTCTTGAGGCCGAACTGCCCGGTGTCGAAGAGAAGCTGCGCACCGCGCTGATCCCGCGTGACCCGGATGATGCGCGCGA
CACCATCATGGAAATCAAGGCTGGCACCGGCGGCGAAGAAGCCGCACTGTTCGCCGGCGACCTGCTGCGCATGTACACCC
GTTACGCCGAGAAGCGTGGCTGGAGCGTGAACGTGCAGTCCGAGAACACCACTGAGCTCGGCGGCGTCAAGGACGTGCAG
ATTGCCATCCGCGCCAAGGGCACGCCCGCTCCCGAAGACGGCGTGTGGGCTTCGATGAAATACGAGGGCGGCGTACACCG
TGTGCAGCGCATCCCCGTCACCGAATCGCAGGGCCGTATCCAGACTTCCGCCGCGGGCGTCATCGTGTTCCCCGAGGCCG
ACGAGGATGACGACGAGATCGAGATCGATCCGAAAGACCTGAAAATCGACATCTTCATGAGCTCCGGCCCCGGCGGCCAG
TCCGTGAACACCACGTATTCCGCCGTGCGCATGACCCACCTGCCCACCGGCATCACAGTGAACATGCAGGATGAGAAGTC
GCAGATTCAGAACCGTGCCGCCGCGTTGCGCGTGCTGAAGTCCCGCCTGCTGGCCATGAAGCATGAGCAGGAGGCCGCCG
AGGCCGCCGACATGCGTCACTCGCAGGTACGTTCGCTTGATCGCTCCGAGCGAATCCGCACATACAACTTCCCGGAAAAC
CGTATCGTCGACCATCGCACGAATTACAAGGCCTACAACCTTGACGCCGTGCTCGACGGCGACCTGCAGGCCGTCATCGA
TTCCGACATCCAAGCCGACGAAGCCGACCGTCTGGCCAACCAGCAGTGA

Upstream 100 bases:

>100_bases
AGGACTGGCGTTTTTGTATTCTTTGACAAGTTTTGATGCAGCATTGACCTGCGCAATCGAATAATCGAACAGAACAGCAC
CATACAGAAGAAAGTGTGTT

Downstream 100 bases:

>100_bases
TAGTAGCGTGAGCGGGTTTGGCCGGTAGATACAATGCTCGACACACTCTAGGCCGTTCGGTCAAGCCTACGGTCGAGCAT
CGCATTTACCGGCCAAACCC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MADEQFPAAATALEEYQSIEEQMASPEVVSNPDKLRKLGRRHAELGAIVGAYKTWLQVKDDLAAAQEMAGEDADFAEEAK
RLEAELPGVEEKLRTALIPRDPDDARDTIMEIKAGTGGEEAALFAGDLLRMYTRYAEKRGWSVNVQSENTTELGGVKDVQ
IAIRAKGTPAPEDGVWASMKYEGGVHRVQRIPVTESQGRIQTSAAGVIVFPEADEDDDEIEIDPKDLKIDIFMSSGPGGQ
SVNTTYSAVRMTHLPTGITVNMQDEKSQIQNRAAALRVLKSRLLAMKHEQEAAEAADMRHSQVRSLDRSERIRTYNFPEN
RIVDHRTNYKAYNLDAVLDGDLQAVIDSDIQADEADRLANQQ

Sequences:

>Translated_362_residues
MADEQFPAAATALEEYQSIEEQMASPEVVSNPDKLRKLGRRHAELGAIVGAYKTWLQVKDDLAAAQEMAGEDADFAEEAK
RLEAELPGVEEKLRTALIPRDPDDARDTIMEIKAGTGGEEAALFAGDLLRMYTRYAEKRGWSVNVQSENTTELGGVKDVQ
IAIRAKGTPAPEDGVWASMKYEGGVHRVQRIPVTESQGRIQTSAAGVIVFPEADEDDDEIEIDPKDLKIDIFMSSGPGGQ
SVNTTYSAVRMTHLPTGITVNMQDEKSQIQNRAAALRVLKSRLLAMKHEQEAAEAADMRHSQVRSLDRSERIRTYNFPEN
RIVDHRTNYKAYNLDAVLDGDLQAVIDSDIQADEADRLANQQ
>Mature_361_residues
ADEQFPAAATALEEYQSIEEQMASPEVVSNPDKLRKLGRRHAELGAIVGAYKTWLQVKDDLAAAQEMAGEDADFAEEAKR
LEAELPGVEEKLRTALIPRDPDDARDTIMEIKAGTGGEEAALFAGDLLRMYTRYAEKRGWSVNVQSENTTELGGVKDVQI
AIRAKGTPAPEDGVWASMKYEGGVHRVQRIPVTESQGRIQTSAAGVIVFPEADEDDDEIEIDPKDLKIDIFMSSGPGGQS
VNTTYSAVRMTHLPTGITVNMQDEKSQIQNRAAALRVLKSRLLAMKHEQEAAEAADMRHSQVRSLDRSERIRTYNFPENR
IVDHRTNYKAYNLDAVLDGDLQAVIDSDIQADEADRLANQQ

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=275, Percent_Identity=40, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=36.0230547550432, Blast_Score=210, Evalue=2e-54,
Organism=Homo sapiens, GI166795305, Length=179, Percent_Identity=41.340782122905, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1787462, Length=353, Percent_Identity=44.7592067988669, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=35.672514619883, Blast_Score=195, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17542784, Length=298, Percent_Identity=35.2348993288591, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=39.0909090909091, Blast_Score=225, Evalue=7e-60,
Organism=Drosophila melanogaster, GI19921226, Length=310, Percent_Identity=39.0322580645161, Blast_Score=190, Evalue=1e-48,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40040; Mature: 39909

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEQFPAAATALEEYQSIEEQMASPEVVSNPDKLRKLGRRHAELGAIVGAYKTWLQVKD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAAQEMAGEDADFAEEAKRLEAELPGVEEKLRTALIPRDPDDARDTIMEIKAGTGGEE
HHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHEECCCCCCH
AALFAGDLLRMYTRYAEKRGWSVNVQSENTTELGGVKDVQIAIRAKGTPAPEDGVWASMK
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEE
YEGGVHRVQRIPVTESQGRIQTSAAGVIVFPEADEDDDEIEIDPKDLKIDIFMSSGPGGQ
ECCCHHHHEECCCCCCCCCEEECCCCEEEECCCCCCCCCEEECCCCEEEEEEEECCCCCC
SVNTTYSAVRMTHLPTGITVNMQDEKSQIQNRAAALRVLKSRLLAMKHEQEAAEAADMRH
CCCCHHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQVRSLDRSERIRTYNFPENRIVDHRTNYKAYNLDAVLDGDLQAVIDSDIQADEADRLAN
HHHHHHHHHCCCEECCCCCCCCCCCCCCCEEEEECEEECCCHHHHHCCCCCCCHHHHHCC
QQ
CC
>Mature Secondary Structure 
ADEQFPAAATALEEYQSIEEQMASPEVVSNPDKLRKLGRRHAELGAIVGAYKTWLQVKD
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLAAAQEMAGEDADFAEEAKRLEAELPGVEEKLRTALIPRDPDDARDTIMEIKAGTGGEE
HHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHEECCCCCCH
AALFAGDLLRMYTRYAEKRGWSVNVQSENTTELGGVKDVQIAIRAKGTPAPEDGVWASMK
HHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEE
YEGGVHRVQRIPVTESQGRIQTSAAGVIVFPEADEDDDEIEIDPKDLKIDIFMSSGPGGQ
ECCCHHHHEECCCCCCCCCEEECCCCEEEECCCCCCCCCEEECCCCEEEEEEEECCCCCC
SVNTTYSAVRMTHLPTGITVNMQDEKSQIQNRAAALRVLKSRLLAMKHEQEAAEAADMRH
CCCCHHHHEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SQVRSLDRSERIRTYNFPENRIVDHRTNYKAYNLDAVLDGDLQAVIDSDIQADEADRLAN
HHHHHHHHHCCCEECCCCCCCCCCCCCCCEEEEECEEECCCHHHHHCCCCCCCHHHHHCC
QQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA