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The map label for this gene is hemK [H]

Identifier: 213691479

GI number: 213691479

Start: 651117

End: 652001

Strand: Direct

Name: hemK [H]

Synonym: Blon_0576

Alternate gene names: 213691479

Gene position: 651117-652001 (Clockwise)

Preceding gene: 213691478

Following gene: 213691480

Centisome position: 22.99

GC content: 60.79

Gene sequence:

>885_bases
ATGGCTGCTGCCGATGTGATTCGTGATGCCGCCGTGCAATTGCGTGAGGCTGGTATTGAGACGCCTGAGCATGATGCCAA
ACTGCTCCTTGCCGAGGCGGCAGGTGTTGAGTTGCGCGATGTCGACAAAGCTCTGTTGATGGGGGAGGAGCTTGGCACAG
CCGAACGGTTTGCCCGCTTCCAATCCATGCTTGCCCGCCGTGCCAAGCGCGAGCCGTTGCAATACATCACCGGTCATGCG
CCGTTCCGCTATCTCGACCTGAAGGTCGGCCCTGGCGTGTTCATTCCGCGTCCGGAAACCGAAACCGTGGTGCAGGCCGG
TCTTGACTGGCTGACAAGAAACGGCATGATCCATCCTTGTGTGGTCGATCTGTGCGCCGGCTCCGGCGCGATTGGGCTGT
CCGTTGTCAGCGAGGTGCCCGGCAGCCAGGTGTGGGCGGTGGAGCTGTCGCCGAACACCGCCGAATGGACGCGAAGGAAT
CTGTCGGAAACCGCGAAAAAGTACCCATCCATTGCCTCGAATTACCATCTGGAAATCGCGGATGCCACGTCCTTCGCCAC
ATTGGCCCAATTGGACGGCACCGTCGATATCGTCATCACCAACCCGCCCTACGTGCCGCAAACCGACATTCCCGAACAGC
CGGAAGTACGCGACTGGGACCCTGAACTGGCCCTGTACGGCGGTTCGGCGGACGGCACGCTCATCCCTGAGCGCATTATC
GAACGCGCGTACCGACTGCTCAAGCCTGGCGGCGTATTGGTCATGGAGCATGATCTCACTCAAGGTGAGCGTCTGGTGGC
CTATGCCAAGGCCATCGGATTTACCGCGGCCCACACCGGCAAGGATTGGACCGGCCGGGATCGCTATCTGTTCGCGGTTT
CCTGA

Upstream 100 bases:

>100_bases
ACACTCTAGGCCGTTCGGTCAAGCCTACGGTCGAGCATCGCATTTACCGGCCAAACCCTTACATTATGTGATAATTAAGG
CACCGGAAGATAGGGATGTG

Downstream 100 bases:

>100_bases
TATTTTCTAAAAATTTCAGGCTTTTCCTGTCTTTTTTCCGCGTTTTCCCGGAATCTTCTTCTCATTTTCCGCAATTACGC
CGTCTCATAATAAGAGCAGG

Product: modification methylase, HemK family

Products: NA

Alternate protein names: M.MleHemKP [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MAAADVIRDAAVQLREAGIETPEHDAKLLLAEAAGVELRDVDKALLMGEELGTAERFARFQSMLARRAKREPLQYITGHA
PFRYLDLKVGPGVFIPRPETETVVQAGLDWLTRNGMIHPCVVDLCAGSGAIGLSVVSEVPGSQVWAVELSPNTAEWTRRN
LSETAKKYPSIASNYHLEIADATSFATLAQLDGTVDIVITNPPYVPQTDIPEQPEVRDWDPELALYGGSADGTLIPERII
ERAYRLLKPGGVLVMEHDLTQGERLVAYAKAIGFTAAHTGKDWTGRDRYLFAVS

Sequences:

>Translated_294_residues
MAAADVIRDAAVQLREAGIETPEHDAKLLLAEAAGVELRDVDKALLMGEELGTAERFARFQSMLARRAKREPLQYITGHA
PFRYLDLKVGPGVFIPRPETETVVQAGLDWLTRNGMIHPCVVDLCAGSGAIGLSVVSEVPGSQVWAVELSPNTAEWTRRN
LSETAKKYPSIASNYHLEIADATSFATLAQLDGTVDIVITNPPYVPQTDIPEQPEVRDWDPELALYGGSADGTLIPERII
ERAYRLLKPGGVLVMEHDLTQGERLVAYAKAIGFTAAHTGKDWTGRDRYLFAVS
>Mature_293_residues
AAADVIRDAAVQLREAGIETPEHDAKLLLAEAAGVELRDVDKALLMGEELGTAERFARFQSMLARRAKREPLQYITGHAP
FRYLDLKVGPGVFIPRPETETVVQAGLDWLTRNGMIHPCVVDLCAGSGAIGLSVVSEVPGSQVWAVELSPNTAEWTRRNL
SETAKKYPSIASNYHLEIADATSFATLAQLDGTVDIVITNPPYVPQTDIPEQPEVRDWDPELALYGGSADGTLIPERIIE
RAYRLLKPGGVLVMEHDLTQGERLVAYAKAIGFTAAHTGKDWTGRDRYLFAVS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=298, Percent_Identity=27.8523489932886, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1787463, Length=284, Percent_Identity=32.0422535211268, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87082085, Length=206, Percent_Identity=27.6699029126214, Blast_Score=77, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24582226, Length=251, Percent_Identity=29.8804780876494, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32133; Mature: 32002

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAADVIRDAAVQLREAGIETPEHDAKLLLAEAAGVELRDVDKALLMGEELGTAERFARF
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
QSMLARRAKREPLQYITGHAPFRYLDLKVGPGVFIPRPETETVVQAGLDWLTRNGMIHPC
HHHHHHHHHCCCHHHHCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCHH
VVDLCAGSGAIGLSVVSEVPGSQVWAVELSPNTAEWTRRNLSETAKKYPSIASNYHLEIA
HHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DATSFATLAQLDGTVDIVITNPPYVPQTDIPEQPEVRDWDPELALYGGSADGTLIPERII
CCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHH
ERAYRLLKPGGVLVMEHDLTQGERLVAYAKAIGFTAAHTGKDWTGRDRYLFAVS
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
AAADVIRDAAVQLREAGIETPEHDAKLLLAEAAGVELRDVDKALLMGEELGTAERFARF
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
QSMLARRAKREPLQYITGHAPFRYLDLKVGPGVFIPRPETETVVQAGLDWLTRNGMIHPC
HHHHHHHHHCCCHHHHCCCCCEEEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCCCHH
VVDLCAGSGAIGLSVVSEVPGSQVWAVELSPNTAEWTRRNLSETAKKYPSIASNYHLEIA
HHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DATSFATLAQLDGTVDIVITNPPYVPQTDIPEQPEVRDWDPELALYGGSADGTLIPERII
CCCCHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHH
ERAYRLLKPGGVLVMEHDLTQGERLVAYAKAIGFTAAHTGKDWTGRDRYLFAVS
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]