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The map label for this gene is tilS [H]

Identifier: 213691452

GI number: 213691452

Start: 618021

End: 619187

Strand: Direct

Name: tilS [H]

Synonym: Blon_0547

Alternate gene names: 213691452

Gene position: 618021-619187 (Clockwise)

Preceding gene: 213691451

Following gene: 213691453

Centisome position: 21.82

GC content: 62.9

Gene sequence:

>1167_bases
ATGGCATATTCCGCACGATTGCGCAAGGCCGTAGGCGCGGTCCGCGCAACGCTGAGCGCTGTGGAACTATGCGATGTGCA
GGCTCCCGAATTCGCGCAGCATGGGGACCATGCCGTTGCCGCCGATGCGCCGCTCGTGCTCGTGGCCTGTTCAGGCGGGC
GCGATTCGATGGCACTGGCCGCGGTGAGCCATATCGTATGCACGTCCATGGGCGTGCGTTGCGGTGCCGTGATTGTGGAT
CACGGCTTGCAGGAGGGTTCCGAACAGGTCGCCGGCGAGGCTGCGAATCGTTGCCGTGCGTTGGGACTGGGCCCGGTCAT
CGTGCGCAACGCAACCGTGCAGGCACGCGGTGAGGGCCTCGAAGCGGCCGCGCGGCAGGCCCGGTACAACGAGCTGTGCG
CCGCGGCGCGTGAATCAGGTGCCATTGCCGTGCTGTTGGCCCACACTATGGACGATCAGGCGGAAACCGTATTGATCGGA
CTGCTGCGCAGCCGTGGCGTGGATGCGTTGGCCGGCATGCCGCAGGTCTTCACACGCAGTGGCGTGACTTTTGCGCGGCC
GCTGCTTACCCTTACTCGAGCCGAGACCACCGGCATCTGTGAAGACTTAGGTGTGGAATATTGGGATGACCCCACCAATG
GCGATGCGGTAGACGGCGAGTTGCCGAACGACTATCCGTTGCGCTCGCGGGTGCGCCATGACCTGCTGCCGGCCATCGAG
CGATTCGCTGGATTCAATGTCGCCCGCCATTTCGCTGAAAGCGCACGGTTGGCCAGAATGGATAAGGAGTATCTGGATCA
GCGATCCGACGAGGTGATGGGGGAGGCGGTTACGACAGTTGATTGGCCGGCATCGTCGGCAGCGGTATCGACCGATACGC
CACGAGCCTGTGCGGCAGGCGATACCAACGATTCGAGCCATGGTGTCGGCCTGATGATCAGCGTCAGACGTATTGCACGT
GAGCCAGAGGCGATTCGGTTGCGGGTCATCGCTCATGCGCTGAGCCAAGCCGGTGTGAACGCCAGTGCCGCCCAGATTGC
CGCCATCGACCGGCTGGTCGTCGATTGGCATGGCCAAGGCGGCGTCTCACTTCCCAGAGGTTATTCAGCCAATCGCAAGA
AACACGTCATTCGCGTGTGCCAAGATGGTGCGCATGCGAATCGCTGA

Upstream 100 bases:

>100_bases
GGGGCTGTGAAAGACCCATCAAGTGCCATCAAAGGCTACAAAGGCCGTAAACGTCCATAAACGCACGAGTACACATAACC
AGAAAAAGGGTGGTGAGTAC

Downstream 100 bases:

>100_bases
CGTTGAGGAAGATATTGACCACGAGCTGGTCAGCAAAGAACAGATCGATGCCAAGATCCACGAGATGGCCGCTCTCGCGA
GCGAGGATTACCGCGATAAG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MAYSARLRKAVGAVRATLSAVELCDVQAPEFAQHGDHAVAADAPLVLVACSGGRDSMALAAVSHIVCTSMGVRCGAVIVD
HGLQEGSEQVAGEAANRCRALGLGPVIVRNATVQARGEGLEAAARQARYNELCAAARESGAIAVLLAHTMDDQAETVLIG
LLRSRGVDALAGMPQVFTRSGVTFARPLLTLTRAETTGICEDLGVEYWDDPTNGDAVDGELPNDYPLRSRVRHDLLPAIE
RFAGFNVARHFAESARLARMDKEYLDQRSDEVMGEAVTTVDWPASSAAVSTDTPRACAAGDTNDSSHGVGLMISVRRIAR
EPEAIRLRVIAHALSQAGVNASAAQIAAIDRLVVDWHGQGGVSLPRGYSANRKKHVIRVCQDGAHANR

Sequences:

>Translated_388_residues
MAYSARLRKAVGAVRATLSAVELCDVQAPEFAQHGDHAVAADAPLVLVACSGGRDSMALAAVSHIVCTSMGVRCGAVIVD
HGLQEGSEQVAGEAANRCRALGLGPVIVRNATVQARGEGLEAAARQARYNELCAAARESGAIAVLLAHTMDDQAETVLIG
LLRSRGVDALAGMPQVFTRSGVTFARPLLTLTRAETTGICEDLGVEYWDDPTNGDAVDGELPNDYPLRSRVRHDLLPAIE
RFAGFNVARHFAESARLARMDKEYLDQRSDEVMGEAVTTVDWPASSAAVSTDTPRACAAGDTNDSSHGVGLMISVRRIAR
EPEAIRLRVIAHALSQAGVNASAAQIAAIDRLVVDWHGQGGVSLPRGYSANRKKHVIRVCQDGAHANR
>Mature_387_residues
AYSARLRKAVGAVRATLSAVELCDVQAPEFAQHGDHAVAADAPLVLVACSGGRDSMALAAVSHIVCTSMGVRCGAVIVDH
GLQEGSEQVAGEAANRCRALGLGPVIVRNATVQARGEGLEAAARQARYNELCAAARESGAIAVLLAHTMDDQAETVLIGL
LRSRGVDALAGMPQVFTRSGVTFARPLLTLTRAETTGICEDLGVEYWDDPTNGDAVDGELPNDYPLRSRVRHDLLPAIER
FAGFNVARHFAESARLARMDKEYLDQRSDEVMGEAVTTVDWPASSAAVSTDTPRACAAGDTNDSSHGVGLMISVRRIARE
PEAIRLRVIAHALSQAGVNASAAQIAAIDRLVVDWHGQGGVSLPRGYSANRKKHVIRVCQDGAHANR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=171, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 41053; Mature: 40922

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSARLRKAVGAVRATLSAVELCDVQAPEFAQHGDHAVAADAPLVLVACSGGRDSMALA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECCCCEEEEEECCCCCHHHHH
AVSHIVCTSMGVRCGAVIVDHGLQEGSEQVAGEAANRCRALGLGPVIVRNATVQARGEGL
HHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCH
EAAARQARYNELCAAARESGAIAVLLAHTMDDQAETVLIGLLRSRGVDALAGMPQVFTRS
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHC
GVTFARPLLTLTRAETTGICEDLGVEYWDDPTNGDAVDGELPNDYPLRSRVRHDLLPAIE
CCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RFAGFNVARHFAESARLARMDKEYLDQRSDEVMGEAVTTVDWPASSAAVSTDTPRACAAG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEECCCCCCCCCCC
DTNDSSHGVGLMISVRRIAREPEAIRLRVIAHALSQAGVNASAAQIAAIDRLVVDWHGQG
CCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCCC
GVSLPRGYSANRKKHVIRVCQDGAHANR
CCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYSARLRKAVGAVRATLSAVELCDVQAPEFAQHGDHAVAADAPLVLVACSGGRDSMALA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEECCCCEEEEEECCCCCHHHHH
AVSHIVCTSMGVRCGAVIVDHGLQEGSEQVAGEAANRCRALGLGPVIVRNATVQARGEGL
HHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCH
EAAARQARYNELCAAARESGAIAVLLAHTMDDQAETVLIGLLRSRGVDALAGMPQVFTRS
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCHHHHCCHHHHHHC
GVTFARPLLTLTRAETTGICEDLGVEYWDDPTNGDAVDGELPNDYPLRSRVRHDLLPAIE
CCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
RFAGFNVARHFAESARLARMDKEYLDQRSDEVMGEAVTTVDWPASSAAVSTDTPRACAAG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEECCCCCCCCCCC
DTNDSSHGVGLMISVRRIAREPEAIRLRVIAHALSQAGVNASAAQIAAIDRLVVDWHGQG
CCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEECCCC
GVSLPRGYSANRKKHVIRVCQDGAHANR
CCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA