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The map label for this gene is lacR [H]

Identifier: 213691421

GI number: 213691421

Start: 584157

End: 584882

Strand: Direct

Name: lacR [H]

Synonym: Blon_0516

Alternate gene names: 213691421

Gene position: 584157-584882 (Clockwise)

Preceding gene: 213691420

Following gene: 213691422

Centisome position: 20.62

GC content: 63.36

Gene sequence:

>726_bases
ATGGCCACGCTCAAGGAGGTCGCGGAACGGGCCGGGGTGTCACAGAGCACGGTGTCGCGCTTGCTGAACGACCCATCGTT
CTCCATCAAGGAGGAGACGCGACGGCGCGTGCTGCGCGTATGCGAGGAGCTGGGATACCGCAACGTGTTCCGTCCCGCGA
TTGCTGTGCTGGACGCTCCTCCATCCGGGGAGAAGCTGCAGGACGCATACTTCTCCGATCTGCGGAAGGTTCTTGCGGAG
TGTGCGGAGTCGATGGAATTGGACCAGCCCACGTTCATCCGGTCCATACGCGAGCTGACGGAACGCGCGGCGGAGTTCGA
TGGGTTCATCACCGTGGATGCCACGGTGTTTCCTGAAGCGGACCTGCGCGCCCTGCATGCGGCACTCCCGCATGGCGTGT
GCATCGACACGAATCCGGCACCGCACCTGTTCGACTCCGTACGGCCCGATCTGTCGCAGACGATGTTGGACGCCCTGGAC
GCGATGATTGCGGCGGGCCGTAGACGCATCGCGTTTCTCGGCGGCGTGGGCAGCATCATGGGCACACATGACTATCCCGC
GAACATCCGCACGTTCAAGACCTGCGCCTACTACCTGCCGCTCGCCGGCATCGTCGCCAGCCTCGTCGTCTTCCTCCTGA
CCGTCAAGATCGACGAGAACATGCACGACCGCATCGTCGAGCAACTCGAAGCGCGCCTCGCCTCCGAGGAAACCGACAAG
CAGTAA

Upstream 100 bases:

>100_bases
ATCAGTTGTAGGTCATTTCCTGAGTAGGAGAATCCGTCATCTTGCAAATCCCGGGTTATGCTGTAGTAAAACCTTTACTA
AAGTTTGCGGGGAGGATGTC

Downstream 100 bases:

>100_bases
GAAATCCAACTACAAACAAAAAGACCTTGGGTGGGTTGTCATCTACAGATGACAACCCACCCACCACTCAAGTCGTTCGA
ATCGTAACTGCGCAATTATT

Product: regulatory protein, LacI

Products: NA

Alternate protein names: Lactose operon repressor [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MATLKEVAERAGVSQSTVSRLLNDPSFSIKEETRRRVLRVCEELGYRNVFRPAIAVLDAPPSGEKLQDAYFSDLRKVLAE
CAESMELDQPTFIRSIRELTERAAEFDGFITVDATVFPEADLRALHAALPHGVCIDTNPAPHLFDSVRPDLSQTMLDALD
AMIAAGRRRIAFLGGVGSIMGTHDYPANIRTFKTCAYYLPLAGIVASLVVFLLTVKIDENMHDRIVEQLEARLASEETDK
Q

Sequences:

>Translated_241_residues
MATLKEVAERAGVSQSTVSRLLNDPSFSIKEETRRRVLRVCEELGYRNVFRPAIAVLDAPPSGEKLQDAYFSDLRKVLAE
CAESMELDQPTFIRSIRELTERAAEFDGFITVDATVFPEADLRALHAALPHGVCIDTNPAPHLFDSVRPDLSQTMLDALD
AMIAAGRRRIAFLGGVGSIMGTHDYPANIRTFKTCAYYLPLAGIVASLVVFLLTVKIDENMHDRIVEQLEARLASEETDK
Q
>Mature_240_residues
ATLKEVAERAGVSQSTVSRLLNDPSFSIKEETRRRVLRVCEELGYRNVFRPAIAVLDAPPSGEKLQDAYFSDLRKVLAEC
AESMELDQPTFIRSIRELTERAAEFDGFITVDATVFPEADLRALHAALPHGVCIDTNPAPHLFDSVRPDLSQTMLDALDA
MIAAGRRRIAFLGGVGSIMGTHDYPANIRTFKTCAYYLPLAGIVASLVVFLLTVKIDENMHDRIVEQLEARLASEETDKQ

Specific function: Negatively regulates the transcription of the lactose utilization genes lacL and lacM [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=192, Percent_Identity=30.2083333333333, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00356 LacI [H]

EC number: NA

Molecular weight: Translated: 26724; Mature: 26593

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKEVAERAGVSQSTVSRLLNDPSFSIKEETRRRVLRVCEELGYRNVFRPAIAVLDAP
CCCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
PSGEKLQDAYFSDLRKVLAECAESMELDQPTFIRSIRELTERAAEFDGFITVDATVFPEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCH
DLRALHAALPHGVCIDTNPAPHLFDSVRPDLSQTMLDALDAMIAAGRRRIAFLGGVGSIM
HHHHHHHHCCCCEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHH
GTHDYPANIRTFKTCAYYLPLAGIVASLVVFLLTVKIDENMHDRIVEQLEARLASEETDK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
Q
C
>Mature Secondary Structure 
ATLKEVAERAGVSQSTVSRLLNDPSFSIKEETRRRVLRVCEELGYRNVFRPAIAVLDAP
CCHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
PSGEKLQDAYFSDLRKVLAECAESMELDQPTFIRSIRELTERAAEFDGFITVDATVFPEA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCH
DLRALHAALPHGVCIDTNPAPHLFDSVRPDLSQTMLDALDAMIAAGRRRIAFLGGVGSIM
HHHHHHHHCCCCEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHCCHHHHH
GTHDYPANIRTFKTCAYYLPLAGIVASLVVFLLTVKIDENMHDRIVEQLEARLASEETDK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788721 [H]