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The map label for this gene is ilvB1 [H]

Identifier: 213691312

GI number: 213691312

Start: 460631

End: 462562

Strand: Reverse

Name: ilvB1 [H]

Synonym: Blon_0399

Alternate gene names: 213691312

Gene position: 462562-460631 (Counterclockwise)

Preceding gene: 213691313

Following gene: 213691311

Centisome position: 16.33

GC content: 64.54

Gene sequence:

>1932_bases
ATGGCTTTACCCACGCCTTTGCAGGCATTCAGCGGTGTGCCGAAAATCAACGCGGCACCCGACCAGCAGACCATCGTCGA
TGGAGAGAAGATGACCGGCGCCCAGGCGCTGGTGCGTTCGCTGGAGGATCTCGGCGTCGAGGACGTCTTCGGCATTCCTG
GCGGCGCGATTCTGCCCGTCTACCACGAGATCAAAGACAACACGAAGTTCCGCTTCGTGCTTATGCGCCACGAGCAGGCC
GCCGGCCACGCCGCCGAAGGCTACGCACTGGCCACTGGCAAGGTGGGCGTGTGCATCGTGACTTCCGGCCCGGGTGCCAC
GAACGTGGTCACGGCCATTGCGGACGCGAATATGGACTCCGTGCCCATGGTCGTGATTACCGGTCAGGTGGGCGTGCAGG
CCATCGGCACTGACGCCTTCCAGGAGGCGGATATCGTGGGCATCACCTACCCGGTGTCCAAGCACTCCTTCCTCGTGACC
CGCGCGCAGGACATTCCTCGCGTGCTTTCCGAGGCGTACTACATCGCCAACACCGGCCGCCCCGGCCCGGTGGTCGTGGA
TCTGACCAAGACCGCACAGACCGGCGACATGTACTACTCATGGCCGCAGCGCATGATTCTGCCCGGCTACAACCCCACCA
CCAAGGCGCACGGCCGTGTGCTGTCCGACGCGGCGAAGCTGTTCTCTCAGTCGTACCGCCCGGTGCTGTATGTGGGTGGC
GGCGCCGCCCGCTCCAACGCCGGCGCACAGGTCAAGGCTCTGGCCGACCTGACCGGCGCCCCCGTGGTCACGACCCTGCC
CGCACGCGGCATCATTCCCGACTCCGACCCGAAGAACCTCGGCATGCTGGGCATGCACGGTACGATTGCCGCAACCGGTG
CCGTGCAGCGCGCTGACCTGCTTGTGGCCATCGGTGCACGTTTCGACGACCGCGTGACCGGCAAGCTCGACGCCTTCGCA
CCGACCGCCCGCGTGATTCATATTGACATCGACCCGGCTGAAATCGGCAAGAACCGTCAGCCCGACGTGCCGATCGTGGG
CGATGCGGCTACCGTGCTCGACGACTTGATTCCCGAAATCCAGCGCACGCAGGCCATTCAGGGCAAGCCGAATCTGGCCC
CTTGGTGGAAGGCGATCGACGGCTGGCGCGAGGAATACCCGATGACGTGGGATGAGCCGACCGACGGATCAATGGCCCCG
CAGTGGGTCATCAAAAAGCTCTCTGAGATGGCCGATCCGTCCACCATCTGGGTGACCGGTGTGGGACAGCACCAGATGTG
GGCTTCGCAGTTCATCGATTTCGAGAACCAGCACGCTTGGATCTCCTCCGGAGGCCTCGGCACGATGGGCTACGGTCTGC
CAGCCGCCATCGGCGCATCCGTGGGCTCGGCCCGCGAGTTCGAGGGCAAGAAGCCGGTGTGGCTGATCGATGGTGACGGT
TCCTTCCAGATGACCTCTGAGGAGCTGGCCGCCGCGTTCCTGGACCACGCTCCGGTGAAGATCGCGATTCTGAACAACTC
CGTGTACGGCATGGTCCGTCAGTGGCAGACGCTGTTCTACGAGCACCACTACTCGCAGACCAACCTGCTCGATGGCGAGG
CGCACGGTGCCGATGGTGCGGCGGCACTGGTCGACGGCGATGCGCCGCTCGAAGTGCCTGATTTCATCAAGTTGGCCGAG
GCATACGGCTGCGTGGGTATCCGCGCGTTCACCGAGGAAGAGGCGATTGCCGCCATCGAAAAGGCGAACCAGATCAACGA
CCGCCCGGTACTGATTGATTTCCGCGTGTGGAAGGATGCGATGGTGTGGCCGATGGTGGCCGCCGGCACCTCCAACGATG
AAGTGACGTATAAGCCCGGCGTCAAGCCGTTGGCCGGCGGTGCGCCCGCACCGGGAACCGGTCCCGATGACGGCGCCGCG
AACGAGCGCTGA

Upstream 100 bases:

>100_bases
CACAAGTCTCGGGGAAGCGACTGGAATCAGACACGCGGGCACCCGGCCCCATCCCAAGGTCGGTCAGGTTCGCTACAAAA
CCTATGAGGTGAGAGGAATC

Downstream 100 bases:

>100_bases
GGAAAGGAGCGTACTCATGGCTAACTATCCTGCATCCCAGCCCGGCTCCCAGCGTCATACGCTGTCCGTGCTGGTGGAGA
ACCGCCCGGGCGTGCTGGCG

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 643; Mature: 642

Protein sequence:

>643_residues
MALPTPLQAFSGVPKINAAPDQQTIVDGEKMTGAQALVRSLEDLGVEDVFGIPGGAILPVYHEIKDNTKFRFVLMRHEQA
AGHAAEGYALATGKVGVCIVTSGPGATNVVTAIADANMDSVPMVVITGQVGVQAIGTDAFQEADIVGITYPVSKHSFLVT
RAQDIPRVLSEAYYIANTGRPGPVVVDLTKTAQTGDMYYSWPQRMILPGYNPTTKAHGRVLSDAAKLFSQSYRPVLYVGG
GAARSNAGAQVKALADLTGAPVVTTLPARGIIPDSDPKNLGMLGMHGTIAATGAVQRADLLVAIGARFDDRVTGKLDAFA
PTARVIHIDIDPAEIGKNRQPDVPIVGDAATVLDDLIPEIQRTQAIQGKPNLAPWWKAIDGWREEYPMTWDEPTDGSMAP
QWVIKKLSEMADPSTIWVTGVGQHQMWASQFIDFENQHAWISSGGLGTMGYGLPAAIGASVGSAREFEGKKPVWLIDGDG
SFQMTSEELAAAFLDHAPVKIAILNNSVYGMVRQWQTLFYEHHYSQTNLLDGEAHGADGAAALVDGDAPLEVPDFIKLAE
AYGCVGIRAFTEEEAIAAIEKANQINDRPVLIDFRVWKDAMVWPMVAAGTSNDEVTYKPGVKPLAGGAPAPGTGPDDGAA
NER

Sequences:

>Translated_643_residues
MALPTPLQAFSGVPKINAAPDQQTIVDGEKMTGAQALVRSLEDLGVEDVFGIPGGAILPVYHEIKDNTKFRFVLMRHEQA
AGHAAEGYALATGKVGVCIVTSGPGATNVVTAIADANMDSVPMVVITGQVGVQAIGTDAFQEADIVGITYPVSKHSFLVT
RAQDIPRVLSEAYYIANTGRPGPVVVDLTKTAQTGDMYYSWPQRMILPGYNPTTKAHGRVLSDAAKLFSQSYRPVLYVGG
GAARSNAGAQVKALADLTGAPVVTTLPARGIIPDSDPKNLGMLGMHGTIAATGAVQRADLLVAIGARFDDRVTGKLDAFA
PTARVIHIDIDPAEIGKNRQPDVPIVGDAATVLDDLIPEIQRTQAIQGKPNLAPWWKAIDGWREEYPMTWDEPTDGSMAP
QWVIKKLSEMADPSTIWVTGVGQHQMWASQFIDFENQHAWISSGGLGTMGYGLPAAIGASVGSAREFEGKKPVWLIDGDG
SFQMTSEELAAAFLDHAPVKIAILNNSVYGMVRQWQTLFYEHHYSQTNLLDGEAHGADGAAALVDGDAPLEVPDFIKLAE
AYGCVGIRAFTEEEAIAAIEKANQINDRPVLIDFRVWKDAMVWPMVAAGTSNDEVTYKPGVKPLAGGAPAPGTGPDDGAA
NER
>Mature_642_residues
ALPTPLQAFSGVPKINAAPDQQTIVDGEKMTGAQALVRSLEDLGVEDVFGIPGGAILPVYHEIKDNTKFRFVLMRHEQAA
GHAAEGYALATGKVGVCIVTSGPGATNVVTAIADANMDSVPMVVITGQVGVQAIGTDAFQEADIVGITYPVSKHSFLVTR
AQDIPRVLSEAYYIANTGRPGPVVVDLTKTAQTGDMYYSWPQRMILPGYNPTTKAHGRVLSDAAKLFSQSYRPVLYVGGG
AARSNAGAQVKALADLTGAPVVTTLPARGIIPDSDPKNLGMLGMHGTIAATGAVQRADLLVAIGARFDDRVTGKLDAFAP
TARVIHIDIDPAEIGKNRQPDVPIVGDAATVLDDLIPEIQRTQAIQGKPNLAPWWKAIDGWREEYPMTWDEPTDGSMAPQ
WVIKKLSEMADPSTIWVTGVGQHQMWASQFIDFENQHAWISSGGLGTMGYGLPAAIGASVGSAREFEGKKPVWLIDGDGS
FQMTSEELAAAFLDHAPVKIAILNNSVYGMVRQWQTLFYEHHYSQTNLLDGEAHGADGAAALVDGDAPLEVPDFIKLAEA
YGCVGIRAFTEEEAIAAIEKANQINDRPVLIDFRVWKDAMVWPMVAAGTSNDEVTYKPGVKPLAGGAPAPGTGPDDGAAN
ER

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=497, Percent_Identity=27.7665995975855, Blast_Score=163, Evalue=6e-40,
Organism=Homo sapiens, GI21361361, Length=533, Percent_Identity=23.6397748592871, Blast_Score=119, Evalue=7e-27,
Organism=Escherichia coli, GI87081685, Length=591, Percent_Identity=42.4703891708968, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1790104, Length=589, Percent_Identity=39.7283531409168, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=559, Percent_Identity=33.0948121645796, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI1787096, Length=584, Percent_Identity=27.3972602739726, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788716, Length=492, Percent_Identity=27.2357723577236, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531299, Length=594, Percent_Identity=26.7676767676768, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17531301, Length=594, Percent_Identity=26.7676767676768, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17542570, Length=546, Percent_Identity=23.992673992674, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323755, Length=630, Percent_Identity=45.3968253968254, Blast_Score=470, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6323163, Length=504, Percent_Identity=24.6031746031746, Blast_Score=94, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6321524, Length=504, Percent_Identity=23.015873015873, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=23.3128834355828, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=507, Percent_Identity=24.0631163708087, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=27.0034843205575, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 68425; Mature: 68294

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPTPLQAFSGVPKINAAPDQQTIVDGEKMTGAQALVRSLEDLGVEDVFGIPGGAILPV
CCCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCHHCCCCCCCCEEHE
YHEIKDNTKFRFVLMRHEQAAGHAAEGYALATGKVGVCIVTSGPGATNVVTAIADANMDS
EHHHCCCCEEEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCCCCEEEEEECCCCCC
VPMVVITGQVGVQAIGTDAFQEADIVGITYPVSKHSFLVTRAQDIPRVLSEAYYIANTGR
CCEEEEECCCCEEEECCCHHCCCCEEEEEECCCCCEEEEECHHHHHHHHHHCEEEECCCC
PGPVVVDLTKTAQTGDMYYSWPQRMILPGYNPTTKAHGRVLSDAAKLFSQSYRPVLYVGG
CCCEEEEEECCCCCCCEEEECCCCEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEEEECC
GAARSNAGAQVKALADLTGAPVVTTLPARGIIPDSDPKNLGMLGMHGTIAATGAVQRADL
CCCCCCCCCEEEEEHHCCCCCEEEECCCCCCCCCCCCCCCCEEECCCCEEECCCHHHCEE
LVAIGARFDDRVTGKLDAFAPTARVIHIDIDPAEIGKNRQPDVPIVGDAATVLDDLIPEI
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCCCEECCHHHHHHHHHHHH
QRTQAIQGKPNLAPWWKAIDGWREEYPMTWDEPTDGSMAPQWVIKKLSEMADPSTIWVTG
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
VGQHQMWASQFIDFENQHAWISSGGLGTMGYGLPAAIGASVGSAREFEGKKPVWLIDGDG
CCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCHHCCCCCCEEEEECCC
SFQMTSEELAAAFLDHAPVKIAILNNSVYGMVRQWQTLFYEHHYSQTNLLDGEAHGADGA
CEEECHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AALVDGDAPLEVPDFIKLAEAYGCVGIRAFTEEEAIAAIEKANQINDRPVLIDFRVWKDA
EEEECCCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCC
MVWPMVAAGTSNDEVTYKPGVKPLAGGAPAPGTGPDDGAANER
HHCEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALPTPLQAFSGVPKINAAPDQQTIVDGEKMTGAQALVRSLEDLGVEDVFGIPGGAILPV
CCCCCHHHHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCHHCCCCCCCCEEHE
YHEIKDNTKFRFVLMRHEQAAGHAAEGYALATGKVGVCIVTSGPGATNVVTAIADANMDS
EHHHCCCCEEEEEEEECCCCCCCCCCCEEEEECCEEEEEEECCCCCCCEEEEEECCCCCC
VPMVVITGQVGVQAIGTDAFQEADIVGITYPVSKHSFLVTRAQDIPRVLSEAYYIANTGR
CCEEEEECCCCEEEECCCHHCCCCEEEEEECCCCCEEEEECHHHHHHHHHHCEEEECCCC
PGPVVVDLTKTAQTGDMYYSWPQRMILPGYNPTTKAHGRVLSDAAKLFSQSYRPVLYVGG
CCCEEEEEECCCCCCCEEEECCCCEEECCCCCCCHHCCHHHHHHHHHHHHCCCCEEEECC
GAARSNAGAQVKALADLTGAPVVTTLPARGIIPDSDPKNLGMLGMHGTIAATGAVQRADL
CCCCCCCCCEEEEEHHCCCCCEEEECCCCCCCCCCCCCCCCEEECCCCEEECCCHHHCEE
LVAIGARFDDRVTGKLDAFAPTARVIHIDIDPAEIGKNRQPDVPIVGDAATVLDDLIPEI
EEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHCCCCCCCCCEECCHHHHHHHHHHHH
QRTQAIQGKPNLAPWWKAIDGWREEYPMTWDEPTDGSMAPQWVIKKLSEMADPSTIWVTG
HHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEE
VGQHQMWASQFIDFENQHAWISSGGLGTMGYGLPAAIGASVGSAREFEGKKPVWLIDGDG
CCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCHHCCCCCCEEEEECCC
SFQMTSEELAAAFLDHAPVKIAILNNSVYGMVRQWQTLFYEHHYSQTNLLDGEAHGADGA
CEEECHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
AALVDGDAPLEVPDFIKLAEAYGCVGIRAFTEEEAIAAIEKANQINDRPVLIDFRVWKDA
EEEECCCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCCEEEEEEEECCCC
MVWPMVAAGTSNDEVTYKPGVKPLAGGAPAPGTGPDDGAANER
HHCEEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]