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The map label for this gene is ribE [H]

Identifier: 213691300

GI number: 213691300

Start: 447744

End: 448373

Strand: Direct

Name: ribE [H]

Synonym: Blon_0387

Alternate gene names: 213691300

Gene position: 447744-448373 (Clockwise)

Preceding gene: 213691299

Following gene: 213691301

Centisome position: 15.81

GC content: 62.22

Gene sequence:

>630_bases
ATGTTCACGGGAATAGTGGAGGAAGTCGGAACGGTGGCGGACATACGGAGGAGCGCCGCCAACTGCACGCTGTCGGTCGA
TGCCACGAACGTGATGGAGGACCTCCATATCGGCGACAGCATGGCGGTGAACGGCATCTGTCTGACGGTCGTCGATTTCG
ACCGGCGGCGCTTCACCGTCGACGTGATGAATGAGACGTGGCGCAGAACCTCGCTTGGCGTATTGCGCCACGGGAGCGGC
GTCAACCTGGAACGGGCCCTGCCGGTGAACGGAAGGTTCGGTGGCCATATCGTCACCGGGCACATCGACGGCACGGGCAG
CATATCCTCCGTGCGGCGCGAAGGCAATGCCGTGTGGTACCGGATCGCGGCGCGGAACGAGATACTCGAAGGCATCGTCG
AGAAAGGCTCAATCGCCGTCGACGGCATCAGCCTGACGGTCGCGGAGGTCACGGAAAGGGACTTCTCGGTATCGATCATC
CCGCACACCTTGGCGCAGACGGTGCTGGGCGAGAAACGGGTCGGCGGCGTGGTCAATCTCGAAAACGACGTATTGGGAAA
ATACGTGCGGAACATGATGACGACCCGGACGGGACTGACGCAGGATCTGCTGTCCCGGTGCGGGTTCTGA

Upstream 100 bases:

>100_bases
GCCAAGGGGTCGCCCTGCCTTCCGACGCCGTCATGCTCCGGCCCCGCGCCTGCTCCCGAGTGGGAGAGGATTATCTGGTG
GAAAGCGAGGTGGTGTATTC

Downstream 100 bases:

>100_bases
GAAAGGATGTATGAATCATGAACCATCAGTCGGTGCAAGCGGCGTTGCAGGCGCTGCGGGAAGGAAAACTCGTTCTGGTC
ATCGATGACGAGGACCGGGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MFTGIVEEVGTVADIRRSAANCTLSVDATNVMEDLHIGDSMAVNGICLTVVDFDRRRFTVDVMNETWRRTSLGVLRHGSG
VNLERALPVNGRFGGHIVTGHIDGTGSISSVRREGNAVWYRIAARNEILEGIVEKGSIAVDGISLTVAEVTERDFSVSII
PHTLAQTVLGEKRVGGVVNLENDVLGKYVRNMMTTRTGLTQDLLSRCGF

Sequences:

>Translated_209_residues
MFTGIVEEVGTVADIRRSAANCTLSVDATNVMEDLHIGDSMAVNGICLTVVDFDRRRFTVDVMNETWRRTSLGVLRHGSG
VNLERALPVNGRFGGHIVTGHIDGTGSISSVRREGNAVWYRIAARNEILEGIVEKGSIAVDGISLTVAEVTERDFSVSII
PHTLAQTVLGEKRVGGVVNLENDVLGKYVRNMMTTRTGLTQDLLSRCGF
>Mature_209_residues
MFTGIVEEVGTVADIRRSAANCTLSVDATNVMEDLHIGDSMAVNGICLTVVDFDRRRFTVDVMNETWRRTSLGVLRHGSG
VNLERALPVNGRFGGHIVTGHIDGTGSISSVRREGNAVWYRIAARNEILEGIVEKGSIAVDGISLTVAEVTERDFSVSII
PHTLAQTVLGEKRVGGVVNLENDVLGKYVRNMMTTRTGLTQDLLSRCGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=39.7849462365591, Blast_Score=125, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319733, Length=205, Percent_Identity=39.0243902439024, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 22688; Mature: 22688

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVEEVGTVADIRRSAANCTLSVDATNVMEDLHIGDSMAVNGICLTVVDFDRRRFTV
CCCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCEEECCEEEEEEECCCCEEEE
DVMNETWRRTSLGVLRHGSGVNLERALPVNGRFGGHIVTGHIDGTGSISSVRREGNAVWY
EHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEEE
RIAARNEILEGIVEKGSIAVDGISLTVAEVTERDFSVSIIPHTLAQTVLGEKRVGGVVNL
EEEHHHHHHHHHHHCCCEEECCEEEEEEECCCCCCEEEECHHHHHHHHHCHHHCCCEEEC
ENDVLGKYVRNMMTTRTGLTQDLLSRCGF
CHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MFTGIVEEVGTVADIRRSAANCTLSVDATNVMEDLHIGDSMAVNGICLTVVDFDRRRFTV
CCCCHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCEEECCEEEEEEECCCCEEEE
DVMNETWRRTSLGVLRHGSGVNLERALPVNGRFGGHIVTGHIDGTGSISSVRREGNAVWY
EHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEEE
RIAARNEILEGIVEKGSIAVDGISLTVAEVTERDFSVSIIPHTLAQTVLGEKRVGGVVNL
EEEHHHHHHHHHHHCCCEEECCEEEEEEECCCCCCEEEECHHHHHHHHHCHHHCCCEEEC
ENDVLGKYVRNMMTTRTGLTQDLLSRCGF
CHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8522537 [H]