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The map label for this gene is ispD [H]

Identifier: 213691269

GI number: 213691269

Start: 409315

End: 410190

Strand: Direct

Name: ispD [H]

Synonym: Blon_0353

Alternate gene names: 213691269

Gene position: 409315-410190 (Clockwise)

Preceding gene: 213691268

Following gene: 213691270

Centisome position: 14.45

GC content: 61.64

Gene sequence:

>876_bases
ATGACTGAGAGAGATTTCGATACCCCCGTCGAGACGCCTACCGTCCAGCCGGCCCCCGCTCAGGGCACCAAACCGGCACA
AACCGTGCCGGTGGTAGCCGTAGTGCTGGCCGCCGGCTTCGGTACGCGCTTCGACCCCGATAATCCCAAGCAGCTTGTCT
CCGTGGGCGGCAAGCCCATCGTGTGCTGGAGCATTGATGCCTTTGAACATTGCGATCGCGTCAGCGACATCGTGGTGGTG
GTCAATCCCAAGGTGCGCGGTGAAGTGGAAACGTTGGTTGGCGAGATGGGCTACACCAAGGTCCGCGTCATCATTGACGG
CGGTGACGAACGTGTGGACAGTACCGCCACCGCGCTCGACATGCTGGCTACGGCTGGCATTCCGGACGATGCCAAGATTC
TGATCCATGACGCCGTCCGTCCGTTCGTGGAACAGTCCGCCATCGACGGTTCGATTGATGCGCTCGACCAGTTCACGGCC
GCGACCGTGGCCTACGCCTCAACCGACACCGTGCTGTTGACCGAGGATCTGGGCGATTTGAAAGTGGTCAAGTCGGTGCC
GGACCGCCCGAACACCTTCCGCGCGCAGACCCCGCAATCCTTCCGCTTCGCGACGATTCGTCACGCTTACGATTTGGCTG
CCGCCGACCCGGACTTCCATCCCACCGACGACACGCGCGTAGTGGTCGATTATCTGCCTGATGAACCCGTCGCCATTGTC
TCCGGTGCGGAAACCAACCTGAAGATCACCACGTTGGAAGATATTCCCACAGCTGAGCGTATCGCCGAGGAGATTCTCGG
TCGTGATCCCAAGGAAGAGGCCCGCGCCCGCATGCATGCCCTGCTGGCCCAAGCAGCCGGCCAGATGCATCGCTAG

Upstream 100 bases:

>100_bases
GCCCTCATCGTGCGTGACTGAACGGAGAATGCGAGCACATTCCGTGGAGAAGATGTGATGCAGTTCACGGACGGCCAGCC
CTCACCCTACGGTGGTGCCT

Downstream 100 bases:

>100_bases
ACTGGCATTATGCGTGAACTCAATGTTGTGATCTCCGGCTTCGACCACTATGACGGCGTGGAGGTCAATCCCGCCGTCGA
AGTGCCCAAGGCCATCGCCG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTERDFDTPVETPTVQPAPAQGTKPAQTVPVVAVVLAAGFGTRFDPDNPKQLVSVGGKPIVCWSIDAFEHCDRVSDIVVV
VNPKVRGEVETLVGEMGYTKVRVIIDGGDERVDSTATALDMLATAGIPDDAKILIHDAVRPFVEQSAIDGSIDALDQFTA
ATVAYASTDTVLLTEDLGDLKVVKSVPDRPNTFRAQTPQSFRFATIRHAYDLAAADPDFHPTDDTRVVVDYLPDEPVAIV
SGAETNLKITTLEDIPTAERIAEEILGRDPKEEARARMHALLAQAAGQMHR

Sequences:

>Translated_291_residues
MTERDFDTPVETPTVQPAPAQGTKPAQTVPVVAVVLAAGFGTRFDPDNPKQLVSVGGKPIVCWSIDAFEHCDRVSDIVVV
VNPKVRGEVETLVGEMGYTKVRVIIDGGDERVDSTATALDMLATAGIPDDAKILIHDAVRPFVEQSAIDGSIDALDQFTA
ATVAYASTDTVLLTEDLGDLKVVKSVPDRPNTFRAQTPQSFRFATIRHAYDLAAADPDFHPTDDTRVVVDYLPDEPVAIV
SGAETNLKITTLEDIPTAERIAEEILGRDPKEEARARMHALLAQAAGQMHR
>Mature_290_residues
TERDFDTPVETPTVQPAPAQGTKPAQTVPVVAVVLAAGFGTRFDPDNPKQLVSVGGKPIVCWSIDAFEHCDRVSDIVVVV
NPKVRGEVETLVGEMGYTKVRVIIDGGDERVDSTATALDMLATAGIPDDAKILIHDAVRPFVEQSAIDGSIDALDQFTAA
TVAYASTDTVLLTEDLGDLKVVKSVPDRPNTFRAQTPQSFRFATIRHAYDLAAADPDFHPTDDTRVVVDYLPDEPVAIVS
GAETNLKITTLEDIPTAERIAEEILGRDPKEEARARMHALLAQAAGQMHR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=254, Percent_Identity=26.3779527559055, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1789104, Length=231, Percent_Identity=31.6017316017316, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 31395; Mature: 31263

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERDFDTPVETPTVQPAPAQGTKPAQTVPVVAVVLAAGFGTRFDPDNPKQLVSVGGKPI
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEE
VCWSIDAFEHCDRVSDIVVVVNPKVRGEVETLVGEMGYTKVRVIIDGGDERVDSTATALD
EEEECHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHH
MLATAGIPDDAKILIHDAVRPFVEQSAIDGSIDALDQFTAATVAYASTDTVLLTEDLGDL
HHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCEEEEECCCCCH
KVVKSVPDRPNTFRAQTPQSFRFATIRHAYDLAAADPDFHPTDDTRVVVDYLPDEPVAIV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEE
SGAETNLKITTLEDIPTAERIAEEILGRDPKEEARARMHALLAQAAGQMHR
ECCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TERDFDTPVETPTVQPAPAQGTKPAQTVPVVAVVLAAGFGTRFDPDNPKQLVSVGGKPI
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEE
VCWSIDAFEHCDRVSDIVVVVNPKVRGEVETLVGEMGYTKVRVIIDGGDERVDSTATALD
EEEECHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHH
MLATAGIPDDAKILIHDAVRPFVEQSAIDGSIDALDQFTAATVAYASTDTVLLTEDLGDL
HHHHCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCEEEEECCCCCH
KVVKSVPDRPNTFRAQTPQSFRFATIRHAYDLAAADPDFHPTDDTRVVVDYLPDEPVAIV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEE
SGAETNLKITTLEDIPTAERIAEEILGRDPKEEARARMHALLAQAAGQMHR
ECCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA