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The map label for this gene is trmD [H]

Identifier: 213691266

GI number: 213691266

Start: 405618

End: 406433

Strand: Reverse

Name: trmD [H]

Synonym: Blon_0350

Alternate gene names: 213691266

Gene position: 406433-405618 (Counterclockwise)

Preceding gene: 213691292

Following gene: 213691256

Centisome position: 14.35

GC content: 62.62

Gene sequence:

>816_bases
ATGAAGATCGATATCGTGAGCGTGTTCCCCGAATACTTTGAGGTGATGAACCTCAGCCTGATGGGCAAAGCCCAGGCCAA
GGGATTGTTGGAGATCAAGGCGCATAACCTGCGCGACTGGACCCACGATGTGCATCATTCGGTGGATGACACGCCAGTCG
GCGGCGGTGCCGGCATGGTGATGAAGCCCGAGGTGTGGAGCGAATGCCTGGACGAACTGCTGGGATTCTCGCGGCCGTCT
GACTCCTCCGCCCCATCCGGCACACCGGTGCTCATCTTCCCGAACCCATCGGCCCCGTTGTTTACCCAGCGCGACGCCAC
CGAGCTCAGCCATGCCGACCATCTGCTGTTCGGCTGCGGCCGGTATGAGGGCTACGATGCGCGCATCCCGGACTACTACC
GCTCCCAGGGCGTCGATGTACGCGAATATTCGATTGGCGACTATGTGCTGAACGGTGGCGAAGTAGCGGTATCGGTGATG
CTTGAGGCCATCACCCGCCTGATGCCCGGGTTCATGGGCAATCCCGATTCGATCGTGGAGGAGTCCTATACCGGCGAGGG
CGCACTGCTTGAACATCGCCAGTACACCAAGCCCGCCGTGTGGCGTGGTATTGCGGTGCCGGACGTGCTGTTGTCCGGCG
ACCACGGCAAGGTGGACCGTTTTCGCCGCGACGAAGCGCTGGCCCGCACCGCCGAGATTCGGCCAGACCTTATCGCGGCA
CTGGATTGCAAGGCGCTGGATAAGGCCGACCGCAAGACCCTGATGGCCTTGGGCTGGGAGGTCTCCGCCGCCCATCCGCG
CCGGCTCGCCGATTAA

Upstream 100 bases:

>100_bases
GGGCTTTTTGCGGGTGGGGATTCTCATCGTCGTATTCATCACCGCAATAGTGTAGTGGTGGCCGTGTAATGCGGGCGGGG
CTACCGTTAGGCTGGATAAC

Downstream 100 bases:

>100_bases
TTCACCACACGGTGGGAGATTCACTACGCGGCGGAATCGTCGGAACGCTCTATATCCGAGTCGTCATCCGCGGATTCGAT
GTAGAAGACGGCGGTTTCGC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKIDIVSVFPEYFEVMNLSLMGKAQAKGLLEIKAHNLRDWTHDVHHSVDDTPVGGGAGMVMKPEVWSECLDELLGFSRPS
DSSAPSGTPVLIFPNPSAPLFTQRDATELSHADHLLFGCGRYEGYDARIPDYYRSQGVDVREYSIGDYVLNGGEVAVSVM
LEAITRLMPGFMGNPDSIVEESYTGEGALLEHRQYTKPAVWRGIAVPDVLLSGDHGKVDRFRRDEALARTAEIRPDLIAA
LDCKALDKADRKTLMALGWEVSAAHPRRLAD

Sequences:

>Translated_271_residues
MKIDIVSVFPEYFEVMNLSLMGKAQAKGLLEIKAHNLRDWTHDVHHSVDDTPVGGGAGMVMKPEVWSECLDELLGFSRPS
DSSAPSGTPVLIFPNPSAPLFTQRDATELSHADHLLFGCGRYEGYDARIPDYYRSQGVDVREYSIGDYVLNGGEVAVSVM
LEAITRLMPGFMGNPDSIVEESYTGEGALLEHRQYTKPAVWRGIAVPDVLLSGDHGKVDRFRRDEALARTAEIRPDLIAA
LDCKALDKADRKTLMALGWEVSAAHPRRLAD
>Mature_271_residues
MKIDIVSVFPEYFEVMNLSLMGKAQAKGLLEIKAHNLRDWTHDVHHSVDDTPVGGGAGMVMKPEVWSECLDELLGFSRPS
DSSAPSGTPVLIFPNPSAPLFTQRDATELSHADHLLFGCGRYEGYDARIPDYYRSQGVDVREYSIGDYVLNGGEVAVSVM
LEAITRLMPGFMGNPDSIVEESYTGEGALLEHRQYTKPAVWRGIAVPDVLLSGDHGKVDRFRRDEALARTAEIRPDLIAA
LDCKALDKADRKTLMALGWEVSAAHPRRLAD

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=242, Percent_Identity=37.603305785124, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29884; Mature: 29884

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDIVSVFPEYFEVMNLSLMGKAQAKGLLEIKAHNLRDWTHDVHHSVDDTPVGGGAGMV
CEEEEEHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCE
MKPEVWSECLDELLGFSRPSDSSAPSGTPVLIFPNPSAPLFTQRDATELSHADHLLFGCG
ECHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHEEECC
RYEGYDARIPDYYRSQGVDVREYSIGDYVLNGGEVAVSVMLEAITRLMPGFMGNPDSIVE
CCCCCCCCCCHHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
ESYTGEGALLEHRQYTKPAVWRGIAVPDVLLSGDHGKVDRFRRDEALARTAEIRPDLIAA
HCCCCCCCEECCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LDCKALDKADRKTLMALGWEVSAAHPRRLAD
HCHHHHHHHHHHHHHHHCCEECCCCCCCCCC
>Mature Secondary Structure
MKIDIVSVFPEYFEVMNLSLMGKAQAKGLLEIKAHNLRDWTHDVHHSVDDTPVGGGAGMV
CEEEEEHHHHHHHHHHHHHHCCHHHHCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCE
MKPEVWSECLDELLGFSRPSDSSAPSGTPVLIFPNPSAPLFTQRDATELSHADHLLFGCG
ECHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHHHEEECC
RYEGYDARIPDYYRSQGVDVREYSIGDYVLNGGEVAVSVMLEAITRLMPGFMGNPDSIVE
CCCCCCCCCCHHHHHCCCCEEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
ESYTGEGALLEHRQYTKPAVWRGIAVPDVLLSGDHGKVDRFRRDEALARTAEIRPDLIAA
HCCCCCCCEECCCCCCCHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHH
LDCKALDKADRKTLMALGWEVSAAHPRRLAD
HCHHHHHHHHHHHHHHHCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA