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The map label for this gene is purR [H]

Identifier: 213691256

GI number: 213691256

Start: 394269

End: 394787

Strand: Reverse

Name: purR [H]

Synonym: Blon_0340

Alternate gene names: 213691256

Gene position: 394787-394269 (Counterclockwise)

Preceding gene: 213691266

Following gene: 213691253

Centisome position: 13.94

GC content: 54.91

Gene sequence:

>519_bases
ATGATCGCCACACAATATCTCATATCATTGGGGCATCGACGAATCGCCGTCATCACTGGCCCTACTAATTCACAATCCTC
ACAAGCGCGGCTGAGCGGATACATGGCGACGCTGCAGAAAAGCGGCATCAGCGCGGATCCGCATCTCATCAAGGTCGGCG
ACTACGTATCCGACAAGGCATACAAGGCGGCATGCAATCTCCTGGAAATGAGGGAGCAGGACCGACCGACCGCCATCTTC
GCGTTCAACGATCTCGTCGCGATCAACGTCTATCGCGCCGCCCGACAGCACGGCATCAGTCTGCCCGAACAATTATCGAT
CATCGGTTTCGACGATGTCTATCCTGCGGCAAGCATGTGTCCATCCCTGACGACCATCAGGCAGCCATTCGACCTCATCG
CCAGACAATGCATCAACCTGATTCTCGAAGCGCGGGAAGGAAAGGTGGAACAGAATTACTTCATTTTTCCCACGCAGCTT
GTCGAACGCGAAAGCTGCGTATCGCCAGCACGTCCGTAA

Upstream 100 bases:

>100_bases
GAGTAGCGAAAATTTCTCTACTCTATATGTAACGCTTTTTGCAGCGTCGCATACGAAAAGAGAAGAGCCGGGGGTAGGCA
TTGATAACTGGACCGGGGGA

Downstream 100 bases:

>100_bases
CTCAGCAACGGAACACAGAATCAGCTTCCACGAAGCACCCATGCATATGCAATCCGTCTCTGGCACAAGCATTTACTTCT
GTTTACCAGATGAAAATACA

Product: LacI-family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MIATQYLISLGHRRIAVITGPTNSQSSQARLSGYMATLQKSGISADPHLIKVGDYVSDKAYKAACNLLEMREQDRPTAIF
AFNDLVAINVYRAARQHGISLPEQLSIIGFDDVYPAASMCPSLTTIRQPFDLIARQCINLILEAREGKVEQNYFIFPTQL
VERESCVSPARP

Sequences:

>Translated_172_residues
MIATQYLISLGHRRIAVITGPTNSQSSQARLSGYMATLQKSGISADPHLIKVGDYVSDKAYKAACNLLEMREQDRPTAIF
AFNDLVAINVYRAARQHGISLPEQLSIIGFDDVYPAASMCPSLTTIRQPFDLIARQCINLILEAREGKVEQNYFIFPTQL
VERESCVSPARP
>Mature_172_residues
MIATQYLISLGHRRIAVITGPTNSQSSQARLSGYMATLQKSGISADPHLIKVGDYVSDKAYKAACNLLEMREQDRPTAIF
AFNDLVAINVYRAARQHGISLPEQLSIIGFDDVYPAASMCPSLTTIRQPFDLIARQCINLILEAREGKVEQNYFIFPTQL
VERESCVSPARP

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=167, Percent_Identity=34.7305389221557, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI1790194, Length=166, Percent_Identity=36.144578313253, Blast_Score=103, Evalue=6e-24,
Organism=Escherichia coli, GI1790369, Length=146, Percent_Identity=37.6712328767123, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786540, Length=168, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1789068, Length=169, Percent_Identity=31.3609467455621, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788474, Length=169, Percent_Identity=31.9526627218935, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789202, Length=171, Percent_Identity=30.9941520467836, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1787580, Length=145, Percent_Identity=30.3448275862069, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1789456, Length=156, Percent_Identity=26.2820512820513, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 19090; Mature: 19090

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATQYLISLGHRRIAVITGPTNSQSSQARLSGYMATLQKSGISADPHLIKVGDYVSDKA
CCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHH
YKAACNLLEMREQDRPTAIFAFNDLVAINVYRAARQHGISLPEQLSIIGFDDVYPAASMC
HHHHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHHHHCCCCCHHEEEEECCCCCCHHHHC
PSLTTIRQPFDLIARQCINLILEAREGKVEQNYFIFPTQLVERESCVSPARP
CCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIATQYLISLGHRRIAVITGPTNSQSSQARLSGYMATLQKSGISADPHLIKVGDYVSDKA
CCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHH
YKAACNLLEMREQDRPTAIFAFNDLVAINVYRAARQHGISLPEQLSIIGFDDVYPAASMC
HHHHHHHHHHHHCCCCEEEEEECCEEEHHHHHHHHHHCCCCCHHEEEEECCCCCCHHHHC
PSLTTIRQPFDLIARQCINLILEAREGKVEQNYFIFPTQLVERESCVSPARP
CCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA