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The map label for this gene is ogt [H]

Identifier: 213691247

GI number: 213691247

Start: 383319

End: 383813

Strand: Reverse

Name: ogt [H]

Synonym: Blon_0330

Alternate gene names: 213691247

Gene position: 383813-383319 (Counterclockwise)

Preceding gene: 213691248

Following gene: 213691246

Centisome position: 13.55

GC content: 63.23

Gene sequence:

>495_bases
ATGGCCTCGGACGGCGAGGGACTTACCGAACTCTGTCTTGAAGACTGGTGGTGGGCCACCGATCACCAAGCCGTTCTCGA
TTCGAATCGCGAATCCGATCGTGAACCCGATGTGTTCCGTATGACCCGCGTATGGCTGACCGCCTATTTCGCCGGCGAGC
GCCCCGGCCTAGATCAGACGCCGCCGTTGCATCCCGCCGGCACGCCATTCCAACATGAAGTGTGGAATCTGGTGGCCGAA
ATTCCCTATGGCATGACGGTAAGTTACGGCGAACTCGCCGCCGAATTGGCCGAACGACGCGGTGGCGGCCGCATGGCCGC
GCAGGCGGTCGGAGGCGCAGTGAAGCGCAATCCGGTTACCATCATCGTGCCCTGTCACCGCGTAGTCGGCTCGGGCCGCG
CGTTTGGCGGCTACGGCGGCCGACTCGACATCAAGGCCGAGTTGCTTGAACTCGAGGGCGTGGATCTGGCGCGCTTCGAT
CTGCCGAACCCTTGA

Upstream 100 bases:

>100_bases
GGCACAGTATGGGCTTCATCCCTCAACGCGCGATAATGGCGGTATGACGCAACGACAACTGATGCGGGCCACAGTCGAAA
CGCCCATCGGCAGGATGATG

Downstream 100 bases:

>100_bases
CGGGCCCAGCGGTCCGCCTCGTCAAAAGGATTGAGTGGTTAACCCTCCCGCCGGCGGGAGATGGCGCGTGCGTCGTAGGG
TGGTCGGCTGGCTGGCATCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MASDGEGLTELCLEDWWWATDHQAVLDSNRESDREPDVFRMTRVWLTAYFAGERPGLDQTPPLHPAGTPFQHEVWNLVAE
IPYGMTVSYGELAAELAERRGGGRMAAQAVGGAVKRNPVTIIVPCHRVVGSGRAFGGYGGRLDIKAELLELEGVDLARFD
LPNP

Sequences:

>Translated_164_residues
MASDGEGLTELCLEDWWWATDHQAVLDSNRESDREPDVFRMTRVWLTAYFAGERPGLDQTPPLHPAGTPFQHEVWNLVAE
IPYGMTVSYGELAAELAERRGGGRMAAQAVGGAVKRNPVTIIVPCHRVVGSGRAFGGYGGRLDIKAELLELEGVDLARFD
LPNP
>Mature_163_residues
ASDGEGLTELCLEDWWWATDHQAVLDSNRESDREPDVFRMTRVWLTAYFAGERPGLDQTPPLHPAGTPFQHEVWNLVAEI
PYGMTVSYGELAAELAERRGGGRMAAQAVGGAVKRNPVTIIVPCHRVVGSGRAFGGYGGRLDIKAELLELEGVDLARFDL
PNP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=130, Percent_Identity=38.4615384615385, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787596, Length=111, Percent_Identity=45.045045045045, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1788542, Length=94, Percent_Identity=41.4893617021277, Blast_Score=67, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=99, Percent_Identity=38.3838383838384, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533068, Length=99, Percent_Identity=38.3838383838384, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320001, Length=86, Percent_Identity=40.6976744186046, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17988; Mature: 17857

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDGEGLTELCLEDWWWATDHQAVLDSNRESDREPDVFRMTRVWLTAYFAGERPGLDQT
CCCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PPLHPAGTPFQHEVWNLVAEIPYGMTVSYGELAAELAERRGGGRMAAQAVGGAVKRNPVT
CCCCCCCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEE
IIVPCHRVVGSGRAFGGYGGRLDIKAELLELEGVDLARFDLPNP
EEEECHHHHCCCCCCCCCCCEEEHEEEEEEECCCEEEECCCCCC
>Mature Secondary Structure 
ASDGEGLTELCLEDWWWATDHQAVLDSNRESDREPDVFRMTRVWLTAYFAGERPGLDQT
CCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCC
PPLHPAGTPFQHEVWNLVAEIPYGMTVSYGELAAELAERRGGGRMAAQAVGGAVKRNPVT
CCCCCCCCCHHHHHHHHHHHCCCCCEECHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEE
IIVPCHRVVGSGRAFGGYGGRLDIKAELLELEGVDLARFDLPNP
EEEECHHHHCCCCCCCCCCCEEEHEEEEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]