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The map label for this gene is nicK [H]

Identifier: 213691209

GI number: 213691209

Start: 347362

End: 347913

Strand: Direct

Name: nicK [H]

Synonym: Blon_0292

Alternate gene names: 213691209

Gene position: 347362-347913 (Clockwise)

Preceding gene: 213691208

Following gene: 213691215

Centisome position: 12.26

GC content: 35.14

Gene sequence:

>552_bases
ATGAATGATAAAAATTTTATTGAAGAATTAAAACATAAACGGAATGAATATGGGGTATCTCAAAGAAGATTTGCCGTTGC
CTGTGGTATCAGTCGTACATATTTTAATCAAATAGAGAACGGTAGTGTTGTTCCCTCTGATGAACTAAAACAAACAATGC
AAAAACAGATTGAGCGTTTTAATCCGCAAGAACCATTATTCTTATTGATTGATTACTTTCGTGTCCGCTTTCCTACTACG
GACGCATTAAAGATTATTCGTGATGTGTTGCAATTAAAATCTGATTATATGCTTTATGAAGATTTTGGCAAGTACGGATA
TGAAAGCAAATATGTTTTAGGCGACATCAACATCATGTGTTCCATGCAAGAGCATTTAGGTGTTTTATTAGAACTAAAAG
GCAGAGGGTGTCGGCAAATGGAAAGTTATCTATTGGCACAGGAACGCTCATGGTATGACTTTATGCTTGATTGTTTAACG
GCAGGTGGTAAGATGAAACGGCTTGACTTGGCAATCAATGATAAAGCAGGAATATTAGATATTCCAAATTAA

Upstream 100 bases:

>100_bases
TTCAAATAAGGAATTGATTGTCTTTTTCTTTTAGGAAAAATTTAAGTAGAGGTAATTATCAGTAAATGGTAGTTGTTTTT
TTGATTGGAGGAATTAAAGA

Downstream 100 bases:

>100_bases
TGGATGACCTTTGTCCTATCGCAAACTTGGGCGAGAGAGAGCTCGGAAGCAGAAGCGAGCTTGTCGTCCTGTTCAATGGC
TAAGACGCTGCTGCCGGGGG

Product: helix-turn-helix domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MNDKNFIEELKHKRNEYGVSQRRFAVACGISRTYFNQIENGSVVPSDELKQTMQKQIERFNPQEPLFLLIDYFRVRFPTT
DALKIIRDVLQLKSDYMLYEDFGKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLDCLT
AGGKMKRLDLAINDKAGILDIPN

Sequences:

>Translated_183_residues
MNDKNFIEELKHKRNEYGVSQRRFAVACGISRTYFNQIENGSVVPSDELKQTMQKQIERFNPQEPLFLLIDYFRVRFPTT
DALKIIRDVLQLKSDYMLYEDFGKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLDCLT
AGGKMKRLDLAINDKAGILDIPN
>Mature_183_residues
MNDKNFIEELKHKRNEYGVSQRRFAVACGISRTYFNQIENGSVVPSDELKQTMQKQIERFNPQEPLFLLIDYFRVRFPTT
DALKIIRDVLQLKSDYMLYEDFGKYGYESKYVLGDINIMCSMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLDCLT
AGGKMKRLDLAINDKAGILDIPN

Specific function: Probable DNA relaxase involved in the transfer of the integrative and conjugative element ICEBs1. Required for the transfer of ICEBs1. Probably mediates conjugation of ICEBs1 by nicking at oriT on the conjugative element and facilitates the translocation

COG id: COG2946

COG function: function code L; Putative phage replication protein RstA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the plasmid replication initiation factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003491 [H]

Pfam domain/function: PF02486 Rep_trans [H]

EC number: NA

Molecular weight: Translated: 21405; Mature: 21405

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDKNFIEELKHKRNEYGVSQRRFAVACGISRTYFNQIENGSVVPSDELKQTMQKQIERF
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
NPQEPLFLLIDYFRVRFPTTDALKIIRDVLQLKSDYMLYEDFGKYGYESKYVLGDINIMC
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECHHHEE
SMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLDCLTAGGKMKRLDLAINDKAGILD
EEHHHCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
IPN
CCC
>Mature Secondary Structure
MNDKNFIEELKHKRNEYGVSQRRFAVACGISRTYFNQIENGSVVPSDELKQTMQKQIERF
CCCHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHC
NPQEPLFLLIDYFRVRFPTTDALKIIRDVLQLKSDYMLYEDFGKYGYESKYVLGDINIMC
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECHHHEE
SMQEHLGVLLELKGRGCRQMESYLLAQERSWYDFMLDCLTAGGKMKRLDLAINDKAGILD
EEHHHCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
IPN
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]