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The map label for this gene is prs [H]

Identifier: 213691154

GI number: 213691154

Start: 279667

End: 280680

Strand: Reverse

Name: prs [H]

Synonym: Blon_0233

Alternate gene names: 213691154

Gene position: 280680-279667 (Counterclockwise)

Preceding gene: 213691160

Following gene: 213691147

Centisome position: 9.91

GC content: 59.86

Gene sequence:

>1014_bases
ATGTCGGCGATTCTTGAAGGCACTCCGGATAAACGACTCGTATTGGTCACGGGCCGCGCCTACCCCGAGCTAGCTGAGAA
GGTGGCCGAATGCTTGGGAACCGAAATCCTCGAAACCACGTCCTATGACTTTGCCAATGGCGAGATGTACGTGCGTTTCA
CCGAACCGGTGCGTGGTGCGGACGTGTTCGTGCTGCAGTGTCATTCGGGCGATATCAACAAGTGGATCATGGAACAGCTC
ATCATGATCGACGCTTTGAAGCGCGCCTCCGCCAAGTCGATTACCGTGGTGGCGCCCTTCCTCGGCTACTCGCGCCAGGA
TAAGAAGCACCGCGGTCGTGAGCCCATCACCGCCCGTCTGATTTTCGATCTGTTCCACGCCGCCGGTGCCGACCGCATTA
TGACGGTCGATCTGCACGCCGCTCAGGAGCAGGGTTTCTTCGATGGTCCGGTCGATCATCTAACCGCCATGCCGGTGCTG
CTCGATTACGTGCGTTCCACCCTTGATCTGGGCAACACCACCATCGTGTCCCCGGATGCCGGCCGTATCAAGGTGTCCGA
ACAGTGGGCCGCCAAGCTAGGCAATCTGCCGCTGGCGTTCATCCACAAACAGCGTGATATCACCCGCCCGAACCATGCCG
AGGCACATGGCATTATCGGCGATGTCGACGGCCGCAACTGCGTGGTCATCGACGACATGGTCGATACCGCCGGTACTATC
TGCGAAGCCGTGCGCACACTGAACGAATCCGGCGCCAAGTCGGTGACCTTGGTCGCCACTCATGGCTTGCTTTCCGGCCC
GGCCGTGGAGCGACTGGCCAACTGCGGCGCGCATGAAATCGTGTTTATGGACACCGTGCCGATTCCTGAAGAGAAGCGCC
TGCCGAATATGACCGTGCTGTCTTGCGCACCGCTGCTGGCCGCGGGTATTCGCTCCGTGTTCGAGGAAGGTTCTGTGGCG
ATTCTGCTCGACGGCCTGCCCGAAGACATGCGTCCGCGCAATATCTATGCGTGA

Upstream 100 bases:

>100_bases
CACTCTTACAACACAGCCCCACTTCACGGCACGCGCTGCGGTGGGGTGCTAGTACACTGGTTAAGGCTAAAAACACTGCA
ACACAAAAGGATGCGAAAGC

Downstream 100 bases:

>100_bases
TTGATACGCCGAAAATCCTTACGAAAAGACGCCGCTTGGCTATTGATCAAGCGGCGTTTTTCCGTATAGCTCCAATCAGA
TGAAAGCGCGCTCGCCGAAG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSAILEGTPDKRLVLVTGRAYPELAEKVAECLGTEILETTSYDFANGEMYVRFTEPVRGADVFVLQCHSGDINKWIMEQL
IMIDALKRASAKSITVVAPFLGYSRQDKKHRGREPITARLIFDLFHAAGADRIMTVDLHAAQEQGFFDGPVDHLTAMPVL
LDYVRSTLDLGNTTIVSPDAGRIKVSEQWAAKLGNLPLAFIHKQRDITRPNHAEAHGIIGDVDGRNCVVIDDMVDTAGTI
CEAVRTLNESGAKSVTLVATHGLLSGPAVERLANCGAHEIVFMDTVPIPEEKRLPNMTVLSCAPLLAAGIRSVFEEGSVA
ILLDGLPEDMRPRNIYA

Sequences:

>Translated_337_residues
MSAILEGTPDKRLVLVTGRAYPELAEKVAECLGTEILETTSYDFANGEMYVRFTEPVRGADVFVLQCHSGDINKWIMEQL
IMIDALKRASAKSITVVAPFLGYSRQDKKHRGREPITARLIFDLFHAAGADRIMTVDLHAAQEQGFFDGPVDHLTAMPVL
LDYVRSTLDLGNTTIVSPDAGRIKVSEQWAAKLGNLPLAFIHKQRDITRPNHAEAHGIIGDVDGRNCVVIDDMVDTAGTI
CEAVRTLNESGAKSVTLVATHGLLSGPAVERLANCGAHEIVFMDTVPIPEEKRLPNMTVLSCAPLLAAGIRSVFEEGSVA
ILLDGLPEDMRPRNIYA
>Mature_336_residues
SAILEGTPDKRLVLVTGRAYPELAEKVAECLGTEILETTSYDFANGEMYVRFTEPVRGADVFVLQCHSGDINKWIMEQLI
MIDALKRASAKSITVVAPFLGYSRQDKKHRGREPITARLIFDLFHAAGADRIMTVDLHAAQEQGFFDGPVDHLTAMPVLL
DYVRSTLDLGNTTIVSPDAGRIKVSEQWAAKLGNLPLAFIHKQRDITRPNHAEAHGIIGDVDGRNCVVIDDMVDTAGTIC
EAVRTLNESGAKSVTLVATHGLLSGPAVERLANCGAHEIVFMDTVPIPEEKRLPNMTVLSCAPLLAAGIRSVFEEGSVAI
LLDGLPEDMRPRNIYA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=317, Percent_Identity=40.6940063091483, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=40.9523809523809, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI84875539, Length=320, Percent_Identity=40.3125, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI28557709, Length=315, Percent_Identity=40.3174603174603, Blast_Score=231, Evalue=5e-61,
Organism=Homo sapiens, GI4506133, Length=335, Percent_Identity=33.7313432835821, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194018537, Length=339, Percent_Identity=33.6283185840708, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI310128524, Length=148, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI310115209, Length=148, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI310118259, Length=148, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI310119946, Length=148, Percent_Identity=28.3783783783784, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1787458, Length=318, Percent_Identity=42.7672955974843, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI25149168, Length=315, Percent_Identity=39.3650793650794, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17554704, Length=315, Percent_Identity=39.3650793650794, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17554702, Length=315, Percent_Identity=39.3650793650794, Blast_Score=228, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71989924, Length=315, Percent_Identity=39.3650793650794, Blast_Score=227, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=32.2485207100592, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319403, Length=316, Percent_Identity=41.4556962025316, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=40.9523809523809, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6321776, Length=321, Percent_Identity=38.9408099688474, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=39.7959183673469, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324511, Length=95, Percent_Identity=38.9473684210526, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=39.3650793650794, Blast_Score=232, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=36.9822485207101, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24651458, Length=362, Percent_Identity=31.767955801105, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24651456, Length=362, Percent_Identity=31.767955801105, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI281362873, Length=362, Percent_Identity=31.767955801105, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24651454, Length=362, Percent_Identity=31.767955801105, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24651462, Length=222, Percent_Identity=36.4864864864865, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24651464, Length=222, Percent_Identity=36.4864864864865, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI45552010, Length=222, Percent_Identity=36.4864864864865, Blast_Score=134, Evalue=8e-32,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 36742; Mature: 36610

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAILEGTPDKRLVLVTGRAYPELAEKVAECLGTEILETTSYDFANGEMYVRFTEPVRGA
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHCCCCCCCCEEEEEECCCCCCC
DVFVLQCHSGDINKWIMEQLIMIDALKRASAKSITVVAPFLGYSRQDKKHRGREPITARL
EEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHCCCCCHHHHH
IFDLFHAAGADRIMTVDLHAAQEQGFFDGPVDHLTAMPVLLDYVRSTLDLGNTTIVSPDA
HHHHHHHCCCCEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GRIKVSEQWAAKLGNLPLAFIHKQRDITRPNHAEAHGIIGDVDGRNCVVIDDMVDTAGTI
CEEEECHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEECCHHHHHHHH
CEAVRTLNESGAKSVTLVATHGLLSGPAVERLANCGAHEIVFMDTVPIPEEKRLPNMTVL
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHCCCCCHHH
SCAPLLAAGIRSVFEEGSVAILLDGLPEDMRPRNIYA
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SAILEGTPDKRLVLVTGRAYPELAEKVAECLGTEILETTSYDFANGEMYVRFTEPVRGA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHCCCCCCCCEEEEEECCCCCCC
DVFVLQCHSGDINKWIMEQLIMIDALKRASAKSITVVAPFLGYSRQDKKHRGREPITARL
EEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHCCCCCHHHHH
IFDLFHAAGADRIMTVDLHAAQEQGFFDGPVDHLTAMPVLLDYVRSTLDLGNTTIVSPDA
HHHHHHHCCCCEEEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
GRIKVSEQWAAKLGNLPLAFIHKQRDITRPNHAEAHGIIGDVDGRNCVVIDDMVDTAGTI
CEEEECHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEECCHHHHHHHH
CEAVRTLNESGAKSVTLVATHGLLSGPAVERLANCGAHEIVFMDTVPIPEEKRLPNMTVL
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCHHCCCCCHHH
SCAPLLAAGIRSVFEEGSVAILLDGLPEDMRPRNIYA
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12381787 [H]