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The map label for this gene is hmrM [H]

Identifier: 213691147

GI number: 213691147

Start: 270920

End: 272365

Strand: Reverse

Name: hmrM [H]

Synonym: Blon_0226

Alternate gene names: 213691147

Gene position: 272365-270920 (Counterclockwise)

Preceding gene: 213691154

Following gene: 213691141

Centisome position: 9.61

GC content: 63.28

Gene sequence:

>1446_bases
ATGCGTGTGAGCGTGCATATAGACTCTTCCGACGCCAAAGCCACCAGTTCCGGCGATACCACACGCCGCGATGGCTTCGG
AACCCCGCATGCCGGCGCAAACACAAACACCAGCAACGGCCCGACCGACGCCCGCTCCACCAACCGCCGGATCGTGGCGC
TGGCCCTGCCGACCTTCGGCCAGCTCATCGCCGAACCGACCTTCGTCCTTATCGACACAGCCATCGTCGGCCATATCGGG
GATGCGGCGTTGGCCGGCCTGTCAATCGGCTCCACCATCATCCTCACGGCAGTCGGACTATGCATATTCCTGGCCTACTC
GACCACTGCGCAGGTGGCGCACCTGCTGGGAGCTGGTCGTCGTCGCGAGGGACTGCAGGCAGGCATTGACGGCCTGTGGC
TGGCCTTGAGTATCGGCACCGTGCTGGGATTGGGATTGTTCGCGGCTGCCGAGCCATTATGCCGGGCACTGGGCGGTCAG
GGCGAAGTATTGGAACAAGCCGTGACCTATACGCGCGCAATCGTGCTGGGCGCGCCAGGCATGCTGATGGTATATGCGGC
GAACGGCATTTTCCGAGGTTTGCAGAAGGTTCGTATCACGCTGATCGCTGCGGTGGGCGGCGCGGTGGTGAACACGGTGC
TTGATGTGTTGTTCGTTATTGTGCTGAATTGGGGCATTGCCGGCTCCGGAGTGGCGACGCTGGTGGCTCAGTGGTTCATG
GGGCTGTTCCTGGTAATCCCCGCCATTCTGTGGTCCCGGGCGGATGGTGCGAGTCTGCGGCCTCGTTTGGCCGGTATTGC
GGCAGCCGGGGGCGACGGACTGCCATTGTTCATTCGCACATTGGCGATTCGTGCGGCCATGGTGACTACGGTCGCCTGTG
CCGCGCGCATGGGTACTGCCGTGTTGGCCGGGTTTCAGGCTGTGAACTCCTCTTGGAATTTTGCGATGAACATGCTGGAT
TCCGTGGGCATCGCCGGCCAGACGCTGGTGGCCACAACGCTGGGCGCTGGCAGCGTGCAGCAGGCGCGGCGGCTGACGCG
GGCGACCGGGCGTGCGGGACTGGTCACCGGCGCGGTAATCGGCACGGCTTTTGCGGTAGTCGGCCTGTTCGCCGGGCACT
TCTTCTCCCCTACGCCACATATTCAGACGCTGATTGCCATCGGCATGGTGACGATGGGCATCTTCTTCCCGCTGCAAGGC
TGGATGATGGCAATCGACGGCATTCTTATCGGCGCGCGCGACTACCGCTATCTGGCCGTCACATGCACGCTGACCGCCGT
GGTGTATGTGACGCTCATACTCATACTGGCCAATATGGTGACGCCGGCGCTGACGAGCGACCTGATGCGTACCGCCGTAC
TCTGGGCCGCGTTCAACGTGGTGCTCATGGGCGGGCGCGGCCTGTCCAACGGGCTGCGCATGCGTTCGGACGCATGGATG
AAGTAG

Upstream 100 bases:

>100_bases
TCCACTAAGTTATCCACATACTGGGGATAACTTAGTGGATAGTCTGGGGATAACATTCCGCGTCCACAACAGGTGCTTGC
GCAATGTAGTCAGGCGTGAT

Downstream 100 bases:

>100_bases
GTATCGGCCGCGCAAAGCGCTGGGCGATTCCGTTTACATCGCGGCGCGGGCGGCGGAATACATGCCGCGCACGATTTGTT
CGCGCCACTTGGACCAGCCG

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter; Na(+)/drug antiporter [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MRVSVHIDSSDAKATSSGDTTRRDGFGTPHAGANTNTSNGPTDARSTNRRIVALALPTFGQLIAEPTFVLIDTAIVGHIG
DAALAGLSIGSTIILTAVGLCIFLAYSTTAQVAHLLGAGRRREGLQAGIDGLWLALSIGTVLGLGLFAAAEPLCRALGGQ
GEVLEQAVTYTRAIVLGAPGMLMVYAANGIFRGLQKVRITLIAAVGGAVVNTVLDVLFVIVLNWGIAGSGVATLVAQWFM
GLFLVIPAILWSRADGASLRPRLAGIAAAGGDGLPLFIRTLAIRAAMVTTVACAARMGTAVLAGFQAVNSSWNFAMNMLD
SVGIAGQTLVATTLGAGSVQQARRLTRATGRAGLVTGAVIGTAFAVVGLFAGHFFSPTPHIQTLIAIGMVTMGIFFPLQG
WMMAIDGILIGARDYRYLAVTCTLTAVVYVTLILILANMVTPALTSDLMRTAVLWAAFNVVLMGGRGLSNGLRMRSDAWM
K

Sequences:

>Translated_481_residues
MRVSVHIDSSDAKATSSGDTTRRDGFGTPHAGANTNTSNGPTDARSTNRRIVALALPTFGQLIAEPTFVLIDTAIVGHIG
DAALAGLSIGSTIILTAVGLCIFLAYSTTAQVAHLLGAGRRREGLQAGIDGLWLALSIGTVLGLGLFAAAEPLCRALGGQ
GEVLEQAVTYTRAIVLGAPGMLMVYAANGIFRGLQKVRITLIAAVGGAVVNTVLDVLFVIVLNWGIAGSGVATLVAQWFM
GLFLVIPAILWSRADGASLRPRLAGIAAAGGDGLPLFIRTLAIRAAMVTTVACAARMGTAVLAGFQAVNSSWNFAMNMLD
SVGIAGQTLVATTLGAGSVQQARRLTRATGRAGLVTGAVIGTAFAVVGLFAGHFFSPTPHIQTLIAIGMVTMGIFFPLQG
WMMAIDGILIGARDYRYLAVTCTLTAVVYVTLILILANMVTPALTSDLMRTAVLWAAFNVVLMGGRGLSNGLRMRSDAWM
K
>Mature_481_residues
MRVSVHIDSSDAKATSSGDTTRRDGFGTPHAGANTNTSNGPTDARSTNRRIVALALPTFGQLIAEPTFVLIDTAIVGHIG
DAALAGLSIGSTIILTAVGLCIFLAYSTTAQVAHLLGAGRRREGLQAGIDGLWLALSIGTVLGLGLFAAAEPLCRALGGQ
GEVLEQAVTYTRAIVLGAPGMLMVYAANGIFRGLQKVRITLIAAVGGAVVNTVLDVLFVIVLNWGIAGSGVATLVAQWFM
GLFLVIPAILWSRADGASLRPRLAGIAAAGGDGLPLFIRTLAIRAAMVTTVACAARMGTAVLAGFQAVNSSWNFAMNMLD
SVGIAGQTLVATTLGAGSVQQARRLTRATGRAGLVTGAVIGTAFAVVGLFAGHFFSPTPHIQTLIAIGMVTMGIFFPLQG
WMMAIDGILIGARDYRYLAVTCTLTAVVYVTLILILANMVTPALTSDLMRTAVLWAAFNVVLMGGRGLSNGLRMRSDAWM
K

Specific function: Multidrug efflux pump that functions as a Na(+)/drug antiporter. Confers resistance to norfloxacin, acriflavine, doxorubicin, ethidium bromide, tetraphenylphosphonium chloride, daunomycin, berberine and sodium deoxycholate [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=334, Percent_Identity=28.4431137724551, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790477, Length=380, Percent_Identity=27.1052631578947, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49824; Mature: 49824

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSVHIDSSDAKATSSGDTTRRDGFGTPHAGANTNTSNGPTDARSTNRRIVALALPTFG
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHH
QLIAEPTFVLIDTAIVGHIGDAALAGLSIGSTIILTAVGLCIFLAYSTTAQVAHLLGAGR
HHHCCCCCEEEHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RREGLQAGIDGLWLALSIGTVLGLGLFAAAEPLCRALGGQGEVLEQAVTYTRAIVLGAPG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MLMVYAANGIFRGLQKVRITLIAAVGGAVVNTVLDVLFVIVLNWGIAGSGVATLVAQWFM
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GLFLVIPAILWSRADGASLRPRLAGIAAAGGDGLPLFIRTLAIRAAMVTTVACAARMGTA
HHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAGFQAVNSSWNFAMNMLDSVGIAGQTLVATTLGAGSVQQARRLTRATGRAGLVTGAVI
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
GTAFAVVGLFAGHFFSPTPHIQTLIAIGMVTMGIFFPLQGWMMAIDGILIGARDYRYLAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
TCTLTAVVYVTLILILANMVTPALTSDLMRTAVLWAAFNVVLMGGRGLSNGLRMRSDAWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEECCCCCC
K
C
>Mature Secondary Structure
MRVSVHIDSSDAKATSSGDTTRRDGFGTPHAGANTNTSNGPTDARSTNRRIVALALPTFG
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHH
QLIAEPTFVLIDTAIVGHIGDAALAGLSIGSTIILTAVGLCIFLAYSTTAQVAHLLGAGR
HHHCCCCCEEEHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RREGLQAGIDGLWLALSIGTVLGLGLFAAAEPLCRALGGQGEVLEQAVTYTRAIVLGAPG
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MLMVYAANGIFRGLQKVRITLIAAVGGAVVNTVLDVLFVIVLNWGIAGSGVATLVAQWFM
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GLFLVIPAILWSRADGASLRPRLAGIAAAGGDGLPLFIRTLAIRAAMVTTVACAARMGTA
HHHHHHHHHHHCCCCCCCCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLAGFQAVNSSWNFAMNMLDSVGIAGQTLVATTLGAGSVQQARRLTRATGRAGLVTGAVI
HHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHH
GTAFAVVGLFAGHFFSPTPHIQTLIAIGMVTMGIFFPLQGWMMAIDGILIGARDYRYLAV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEH
TCTLTAVVYVTLILILANMVTPALTSDLMRTAVLWAAFNVVLMGGRGLSNGLRMRSDAWM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEECCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]